Antibacterial properties of certain essential oils against different strains of Staphylococcus aureus

Nedorostova, Lenka; Klouček, Pavel; Smíd, Jakub; Urban, Jan; Kokoška, Ladislav; Štolcová, M.

doi:10.1055/s-0029-1234932

Cited by 2 publications

(1 citation statement)

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“…All plates were left for 30min at room temperature to allow the absorption of the fraction and then incubated at 37 o C for 24 h. After the incubation period, the mean diameter of inhibition halo was measured in millimeters [25]. Depending on the size of the zone and adopted assessment criteria according to [26] microorganisms were de ned as sensitive or resistant.…”

Section: Antibacterial Susceptiblity Testing On the Bioactive Fractionsmentioning

confidence: 99%

Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

Ojo

Udewena

Adetunji

2022

GA – 70th Annual Meeting 2022

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BackgroundGroup B Streptococcus (GBS) is a harmless commensal bacterium in healthy adults but it causes sepsis in neonates resulting in a high rate of mortality. This study was carried out to investigate the antibacterial activity of Bryophyllum pinnatum and Rauvol a vomitoria bioactive fractions on thirty-ve multidrug resistant Group B Streptococcus (GBS) strains implicated on neonatal sepsis as well as to identify the antibiotic resistant genes present. The phytochemical analyses were carried out using column chromatography. Antibacterial activity, Minimum Inhibitory Concentration (MIC) and Minimum Bacteria Concentration (MBC) of the different plant biofractions was determined using disc diffusion technique. The presence of erythromycin (ermB) and tetracycline (tetO) resistant genes was identi ed using duplex PCR techniques. Graphing was done using Graph pad prism (version 5.0a). ResultThe results obtained from this study show that the selected plants have dose dependent activity against GBS. Ethanol biofraction of R. vomitoria had the highest activity with an MIC value ranges between 12.5 mg/ml and 50 mg/ml and MBC ranges from 25 mg/ml and 50 mg/ml followed by methanol biofraction of B. pinnatum with an MIC value ranging between 50mg/ml and 100mg/ml on 32 out of the 35 strains investigated, leaving the other three having MIC values of 25 mg/ml then MBC values ranging between 50 mg/ml and 100mg/ml while N-hexane and aqueous biofractions had the least activity. Also, the presence of ermB and tetO resistant genes were present in all the ten representative GBS strains tested. ConclusionThe high rate of activity shown by the methanol and ethanol extracts of both plants suggests that the plants can serve as a potential alternative for the treatment of neonatal sepsis. However, a further study on their in vivo activity is important in order to evaluate the e ciency, safety and potential adverse effects and drug herb interactions of the plants.

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Section: Antibacterial Susceptiblity Testing On the Bioactive Fractionsmentioning

confidence: 99%

Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

Ojo

Udewena

Adetunji

2022

GA – 70th Annual Meeting 2022

View full text Add to dashboard Cite

show abstract

Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

Ojo

Udewena

Adetunji

2022

Preprint

View full text Add to dashboard Cite

Background Group B Streptococcus (GBS) is a harmless commensal bacterium in healthy adults but it causes sepsis in neonates resulting in a high rate of mortality. This study was carried out to investigate the antibacterial activity of Bryophyllum pinnatum and Rauvolfia vomitoria bioactive fractions on thirty-five multidrug resistant Group B Streptococcus (GBS) strains implicated on neonatal sepsis as well as to identify the antibiotic resistant genes present. The phytochemical analyses were carried out using column chromatography. Antibacterial activity, Minimum Inhibitory Concentration (MIC) and Minimum Bacteria Concentration (MBC) of the different plant biofractions was determined using disc diffusion technique. The presence of erythromycin (ermB) and tetracycline (tetO) resistant genes was identified using duplex PCR techniques. Graphing was done using Graph pad prism (version 5.0a). Result The results obtained from this study show that the selected plants have dose dependent activity against GBS. Ethanol biofraction of R. vomitoria had the highest activity with an MIC value ranges between 12.5 mg/ml and 50 mg/ml and MBC ranges from 25 mg/ml and 50 mg/ml followed by methanol biofraction of B. pinnatum with an MIC value ranging between 50mg/ml and 100mg/ml on 32 out of the 35 strains investigated, leaving the other three having MIC values of 25 mg/ml then MBC values ranging between 50 mg/ml and 100mg/ml while N-hexane and aqueous biofractions had the least activity. Also, the presence of ermB and tetO resistant genes were present in all the ten representative GBS strains tested. Conclusion The high rate of activity shown by the methanol and ethanol extracts of both plants suggests that the plants can serve as a potential alternative for the treatment of neonatal sepsis. However, a further study on their in vivo activity is important in order to evaluate the efficiency, safety and potential adverse effects and drug herb interactions of the plants.

show abstract

Antibacterial properties of certain essential oils against different strains of Staphylococcus aureus

Cited by 2 publications

References 0 publications

Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

Bioactivity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

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