Antibacterial properties and GC-MS analysis of ethyl acetate extracts of Xerophyta spekei (Baker) and Grewia tembensis (Fresen)

Nyalo, Paul Ochieng; Omwenga, George; Ngugi, Mathew Piero

doi:10.1016/j.heliyon.2023.e14461

Cited by 9 publications

(5 citation statements)

References 27 publications

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“…Medicinal plants are known to produce secondary metabolites with known effects against bacterial pathogens and less adverse effects in comparison to conventional antibacterial agents [23]. Natural products from medicinal plants can be a resource for researching new drugs, especially in organic farming areas.…”

Section: Discussionmentioning

confidence: 99%

Chemical components of different solvent extracts of Asclepias curassavica L. and antibacterial effect of the extracts on tomato pathogens

Aktepe,

Ferahoğlu,

Aysan

et al. 2024

BIO Web Conf.

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The use of environmentally friendly and sustainable agricultural methods in the control of diseases and pests is of great importance. In both conventional and organic agricultural production systems, the utilization of various plant extracts as part of integrated pest management has gained significance in recent years. The chemical constituents of various solutions derived from the flowers, leaves, and roots of the Asclepias curassavica L. plant were investigated, along with the possibilities of utilizing these solutions in the control of tomato plant pathogenic bacteria. As a result of the analysis, acetic acid in 60% and 80% ethanol solutions, as well as acetic acid methy ester in 60% and 80% methanol solutions, were detected in the flowers, leaves, and roots. The effects of A. curassavica flower, leaf, and root extracts, prepared using three different solvents (water, methanol, and ethanol), were investigated on eight different pathogenic bacteria (Agrobacterium tumefaciens, Clavibacter michiganensis, Dickeya zeae, Pectobacterium caratovorum, Pseudomonas phaseolicola, Pseudomonas tomato, Pseudomonas viridiflava, and Xanthomonas euvesicatoria) that cause diseases in plants. Several main compounds such as acetic acid, acetic acid. methyl ester, Furfural, 2-Furanmethanol, 4H-Pyran-4-one. 2.3-dihydro-3.5-dihydroxy-6-methyl-, Glycerin, Benzo furan. 2.3-dihydro- and 5-Hydroxy methyl furfural were identified as analyzed by GC-MS with different concentrations of ethanol and methanol solutions used for the flower, leaves and root of A. curassavica plants. The flower extract prepared with 80% ethanol exhibited a higher inhibition zone (ranging from 1.5 mm to 5.3 mm) in all pathogens, compared to other applications. The successful suppression effect of A. curassavica flower extracts on this disease is promising, especially in organic farming areas. Additionally, since it is environmentally friendly and sustainable, it can be included in integrated control methods to prevent the loss of productivity caused by diseases.

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Section: Discussionmentioning

confidence: 99%

Chemical components of different solvent extracts of Asclepias curassavica L. and antibacterial effect of the extracts on tomato pathogens

Aktepe,

Ferahoğlu,

Aysan

et al. 2024

BIO Web Conf.

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“…Grewia species is a source of food, fodder, and firewood and is notably used in traditional medicine to cure several ailments, including rheumatism, diabetes, diarrhea, and heart and blood disorders; protect the liver; cure inflammation; treat fever; and relieve pain [3][4]. A literature survey indicated that the secondary metabolites from the genus Grewia show diverse biological effects, such as antioxidant [5][6][7][8], antimalarial [8][9], antibacterial [10][11][12], antidiabetic [13], anticholinesterase [7], anticancer [8,[14][15][16], antiplasmodial, antileishmanial, and antitrypanosomal activities [17]. Previous studies have examined the anticancer and antioxidant abilities of extracts from some plant species.…”

Section: ■ Introductionmentioning

confidence: 99%

Volatile Organic Compounds and Antioxidant, Cytotoxic Activities of Extracts from the Leaves of <i>Grewia bulot</i>

Pham,

Ho,

et al. 2023

Indones. J. Chem.

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This research aims to determine the volatile compounds present in Grewia bulot leaf extracts and evaluate their cytotoxic and antioxidant activities. The volatile constituents of the n-hexane and dichloromethane extracts were identified by using gas chromatography–mass spectrometry. The main compounds identified in the former were neophytadiene (18.2%), methyl palmitate (14.4%), methyl linoleate (9.7%), β-sitosterol (4.5%), and methyl stearate (3.4%), while those in the latter were palmitic acid (9.8%), hexadecane (7.4%), octadecane (6.0%), neophytadiene (5.3%), and 2-tert-butoxyethanol (5.3%). The cytotoxicities of the extracts were examined against four human cancer cell lines (SK-LU-1, Hep-G2, MCF-7, and KB), while their antioxidant activities were assessed using the DPPH radical scavenging assay. The n-hexane and dichloromethane extracts displayed weak activity against these cancer cell lines, with IC50 values ranging from 90.60 ± 3.49 to 98.27 ± 2.77 µg/mL. All extracts showed antioxidant activities, and the methanol extract exhibited the strongest at an SC50 value of 9.39 ± 0.90 µg/mL. This is the first report on the volatile constituents and bioactivities of G. bulot leaf extracts, suggesting their potential application as antioxidants.

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“…Tis current study aimed to evaluate the in vitro antioxidant and antidiabetic activities of the DCM extract of Xerophyta spekei. Te plant Xerophyta spekei belongs to the family of Velloziaceae and is common in Kenya, Tanzania, Zambia, Zimbabwe, and Ethiopia [22]. Te shrub measures 2-5 m in height by 6-12 cm in thickness, and its leaves are congregated at one edge.…”

Section: Introductionmentioning

confidence: 99%

“…Te plant is also well adapted to dry climates. It is used in South Africa by herbalists to manage pain and infammation [22]. Among the Mbeere and Embu communities, it is used to treat wounds, snake bites, and diabetes mellitus [23].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Hypoglycemic and Antioxidant Activities of Dichloromethane Extract of Xerophyta spekei

Ndile,

Makori,

Kibiti

et al. 2023

Scientifica

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Diabetes mellitus is a chronic metabolic disorder which has greatly led to an increase in morbidity and mortality globally. Although Xerophyta spekei is widely used for the management of diabetes among the Embu and Mbeere communities in Kenya, it has never been empirically evaluated for its hypoglycemic activity. This study was carried out to verify the hypoglycemic activity of dichloromethane (DCM) extract of Xerophyta spekei as well as its antioxidant activity using various in vitro techniques. Phytochemicals associated with its antioxidant activity were identified through GC-MS. Data were subjected to descriptive statistics and expressed as mean ± standard error of the mean (X̄ ± SEM). Comparison between various variables was performed by using unpaired Student’s t-test and one-way analysis of variance (ANOVA), followed by Tukey’s post-hoc test. The confidence interval was set at 95%. The obtained results were presented in tables and graphs. Results showed that there was no difference in α-amylase inhibition activity between the plant extract and the standard (IC50 525.9 ± 12.34 and 475.1 ± 9.115, respectively; p > 0.05). Besides, the glucose adsorption activity of the extract increased with an increase in glucose concentration (from 5.89 to 32.64 mg/dl at 5 mmol and 30 mmol of glucose, respectively; p < 0.05). The extract also limited the diffusion of glucose more than the negative control (7.49 and 17.63 mg/dl, respectively; p < 0.05). It also enhanced glucose uptake by yeast cells. In addition, the studied plant extract showed notable antioxidant activities. The therapeutic effects exhibited by this plant in managing diabetes mellitus and other ailments could be due to its antioxidant as well as its hypoglycemic activity. The study recommends the evaluation of X. spekei for in vivo hypoglycemic and antioxidant activities. Besides, the isolation of bioactive phytochemicals from the plant may lead to the development of new hypoglycaemic agents.

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Antibacterial properties and GC-MS analysis of ethyl acetate extracts of Xerophyta spekei (Baker) and Grewia tembensis (Fresen)

Cited by 9 publications

References 27 publications

Chemical components of different solvent extracts of Asclepias curassavica L. and antibacterial effect of the extracts on tomato pathogens

Chemical components of different solvent extracts of Asclepias curassavica L. and antibacterial effect of the extracts on tomato pathogens

Volatile Organic Compounds and Antioxidant, Cytotoxic Activities of Extracts from the Leaves of <i>Grewia bulot</i>

In Vitro Hypoglycemic and Antioxidant Activities of Dichloromethane Extract of Xerophyta spekei

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