Antibacterial Potentiality of Commercially Available Probiotic Lactobacilli and Curd Lactobacilli Strains, Alone and in Combination, against Human Pathogenic Bacteria

Halder, Debashis; Shyamapada, M

doi:10.21767/2172-0479.100061

Cited by 18 publications

(16 citation statements)

References 36 publications

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“…The lactic acid bacterial strains L. acidophilus , L. plantarum , L. fermentum , L. casei and L. rhamnosus , can effectively be applied against the urinary tract infection causing Proteus species ( P. mirabilis and P. vulgaris ), as has been reported by Goudarzi et al [11]. In the earlier study, utilizing some curd lactobacilli and commercially available lactobacilli strains, we demonstrated, singly and in combination, their antibacterial activity against Klebsiella pneumoniae , and E. coli [12]. …”

Section: Introductionsupporting

confidence: 59%

“…The “ R ” (width of clear zone) values were also determined as per the formula stated earlier [12]:

R = \frac{(d Inhib - d Spot)}{2}

(“ d Inhib”: the diameter of clear zone around the “ d Spot”; and “ d Spot”: the diameter of spot form of lactobacilli grown on MRS agar plate). The scores of growth inhibition of indicator bacteria were considered as no inhibition capacity when “ R ” was <2 mm; low inhibition capacity with “ R ” values of 2–5 mm, and high inhibition capacity with “ R ” values >6 mm [18,19].…”

Section: Methodsmentioning

confidence: 99%

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Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

et al. 2017

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Background: Indigenous lactic acid bacteria are well known probiotics having antibacterial activity against potentially pathogenic bacteria. This study aims to characterize the curd lactobacilli for their probiotic potentiality and antagonistic activity against clinical bacteria. Methods: Four curd samples were processed microbiologically for the isolation of lactic acid bacteria (LAB). The LAB strains obtained were identified by conventional methods: cultural aspect, gram-staining, biochemical and sugar fermentation tests. The probiotic properties were justified with tolerance to low-pH, bile salt and sodium chloride, and the antagonistic activity of the lactobacilli against human pathogenic bacteria (Escherichia coli, Proteus vulgaris, Acinetobacter baumannii and Salmonella enterica serovar Typhi) was assessed. Hemolytic activity and antibiotic susceptibility were determined for the lactobacilli isolates, and the cumulative probiotic potential (CPP) values were recorded. Result: Four lactobacilli isolates, L. animalis LMEM6, L. plantarum LMEM7, L. acidophilus LMEM8 and L. rhamnosus LMEM9, procured from the curd samples, survived in low-pH and high bile salt conditions, and showed growth inhibitory activity against the indicator bacteria by agar-well (zone diameter of inhibition; ZDIs: 13.67 ± 0.58–29.50 ± 2.10 mm) and agar overlay (ZDIs: 11.33 ± 0.58–35.67 ± 2.52 mm) methods; the average growth inhibitory activity of lactobacilli ranged 233.34 ± 45.54–280.56 ± 83.67 AU/mL, against the test bacterial pathogens. All the lactobacilli were non-hemolytic and sensitive to most of the test antibiotics. The CPP values of the isolated LAB were recorded as 80–100%. Conclusion: The curd lactobacilli procured might be used as the valid candidates of probiotics, and bio-therapeutics against bacterial infection to humans.

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Section: Introductionsupporting

confidence: 59%

“…The “ R ” (width of clear zone) values were also determined as per the formula stated earlier [12]:

R = \frac{(d Inhib - d Spot)}{2}

Section: Methodsmentioning

confidence: 99%

Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

et al. 2017

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“…Our results are in agreement to findings of Halder and Shyamapada (2016), who also observed the susceptibility of lactobacilli against cefotaxime. Resistance to cephalosporins can be attributed to the presence of β-lactamases and/or efflux pumps (Pfeifer et al, 2010), or due to cell wall impermeability (Delgado et al, 2005).…”

Section: Resultssupporting

confidence: 93%

In vitro Assessment of Antibiotic Resistance Pattern among Lactobacillus Strains Isolated from Goat Milk

Thakur¹,

Sharma²,

Rokana³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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“…The antibiotic susceptibility of the LAB (LMEM 22) was executed by disc diffusion method (Bauer et al, 1996), the details of which was described before (Halder and Mandal, 2016;Halder et al, 2017). The antibiotic discs (Hi-Media, Mumbai, India) used in the study included amikacin (Ak: 30-μg/disc), amoxyclav (Ac: 30-μg/disc), ampicillin (Am: 10-μg/disc), ciprofl oxacin (Cp: 5-μg/disc), cfoxitin (Cx: 30-μg/disc), cefotaxime (Ct: 30-μg/disc), cefotaxime/clavunilic acid (Cc: 30/10μg/disc), gentamycin (Gm: 30-μg/disc), imipenem (Ip: 10-μg/disc), kanamycin (Km: 30-μg/disc), methicillin (Me: 5-μg/disc), meropenem (Mp: 10-μg/disc), tetracycline (Tc: 30-μg/disc), trimethoprim (Tm: 5-μg/ disc) and vancomycin (Vm: 30-μg/disc).…”

Section: Methodsmentioning

confidence: 99%

Insights into the antagonism of Lactobacillus fermentum curd isolate against Gram-positive and Gram-negative pathogenic bacteria

Halder¹,

Mandal²

2018

Biosci. Biotech. Res. Comm

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This communication characterizes the curd isolate of lactic acid bacteria having the capacity to antagonize human pathogenic bacteria. The commercially available curd, in sealed form in a plastic cup, was procured from Malda town market (West Bengal state, India) and processed microbiologically, using de Man Rogosa Sharpe medium, for the isolation of lactic acid bacteria. The pure bacteria culture obtained was identifi ed, by phenotypic characterization through conventional methods, as Lactobacillus fermentum, and designated as LMEM 22. The Lactobacillus fermentum LMEM 22 curd isolate had mixed antibiotic susceptibility patterns, showing resistance (ZDI: ≤15 mm) to amikacin, ciprofl oxacin, kanamycin, methicillin and vancomycin, sensitivity (ZDI: ≥ 21 mm) to ampicillin, amoxyclav, gentamycin, cefotaxime, imipenem, meropenem and tetracycline, and intermediate susceptibility (ZDI: 16-20 mm) to cfoxitin and trimethoprim. The L. fermentum LMEM22 antagonizes both gram-negative: Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Klebsiella pneumoniae, Salmonella enterica serovar Typhi, and gram-positive: Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Listeria monocytogenes, bacterial pathogens, following agar overlay (ZDI: 17±1.73 mm to 20±1.00 mm, for gram-positive, and 18±2.00 mm to 33±2.65 mm for gram-negative bacteria) as well as agar-well diffusion (ZDI: 10.67±2.08 mm to 12±1.00 mm, for gram-positive, and 13.00±2.65 mm to 18.00±3.00 mm, for gram-negative bacteria) techniques. The overall bacteriocin activity (AU/ml) of Lactobacillus fermentum LMEM 22 for the test bacterial pathogens ranged 142.27-240.00, and the 'R' value ranged 5.5-13.5. This study underlines the usefulness of locally available lactic acid bacteria in designing the probiotic microorganisms for biotherapy.

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Antibacterial Potentiality of Commercially Available Probiotic Lactobacilli and Curd Lactobacilli Strains, Alone and in Combination, against Human Pathogenic Bacteria

Cited by 18 publications

References 36 publications

Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

Indigenous Probiotic Lactobacillus Isolates Presenting Antibiotic like Activity against Human Pathogenic Bacteria

In vitro Assessment of Antibiotic Resistance Pattern among Lactobacillus Strains Isolated from Goat Milk

Insights into the antagonism of Lactobacillus fermentum curd isolate against Gram-positive and Gram-negative pathogenic bacteria

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