Antibacterial Potential of Novel Acetamide Derivatives of 2-Mercaptobenzothiazole: Synthesis and Docking Studies

Abstract

2-Mercaptobenzothiazole and its derivatives are widely known for their diverse biological activities, particularly antimicrobial and anticancer potential. In the present study, a series of new hybrid compounds consisting of 2-mercaptobenzothiazole and different aryl amines 2(a−j) were synthesized and characterized by Fourier transform infrared (FTIR), 1 H NMR, and 13 C NMR spectral data. The synthesized compounds were screened for in vitro antibacterial activities through agar well diffusion assay. Among the series, 2b, 2c, and 2i exhibited significant antibacterial activity comparable to the standard drug levofloxacin. Based on their antibacterial potential, these compounds were further tested for their antibiofilm activity. All of the three compounds showed promising antibiofilm potential, even better than the standard drug cefadroxil at 100 μg/100 μL concentration. Molecular docking studies were performed to explore the antibacterial mechanism of these compounds. Strikingly, the molecule 2i shared the same hydrophobic pockets as those of levofloxacin in case of bacterial kinases and DNA gyrases. In addition, 2i exhibited satisfactory antibiofilm activity in comparison to the standard. Our study therefore suggested that the synthetic compound 2i possesses remarkable antibacterial activity and may serve as a lead molecule for the discovery of potent antibacterial agents.

Magnesium Powder-Catalyzed, Highly Efficient, Solvent-Free Synthesis of Amides through N-Acetylation of Amines and their Antibacterial Activity

“…It also possesses strong apoptotic activity and inhibits the growth of U87 in a dose dependent manner [49]. The antibacterial activity of N-(4-hydroxyphenyl)acetamide (2e) against E. coli has been reported by Jayadevappa et al as well [2].…”

“…Acetamide derivatives are reported to possess several bioactivities such as antimicrobial [1][2][3][4][5], antitubercular [6,7], antimalarial [8], antiparasitic [9], antidiabetic [10,11], antipsychotic [12], antioxidant activities [13], etc. Acetamides are used also as chemotherapeutic agents for inflammation and inflammation-associated cancers [14,15].…”