Antibacterial potential of essential oils of the needles of Pinus halepensis against Staphylococcus aureus and Escherichia coli

Raho, Ghalem Bachir

doi:10.12980/jclm.2.2014apjtd-2014-0041

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…Determination of essential oil compounds from several populations could be used for determining their chemotypes. Several studies have investigated the antibacterial activity of essential oils obtained from different plant parts of P.halepensis (needles, roots, buds or cones) (Hmamouchi et al 2001;Abi Ayed et al 2011;Ghanemi et al 2013;Ghalem 2014;Mohareb et al 2016;Ashmawy et al 2018;Bouyahya et al 2019, Mirtic et al2019. The antibacterial effect of P. halepensis essential oil is by the irreversible damage of bacterial cell wall and membrane, and resulting in leakages of proteins and nucleic acids (DNA & RNA) out of the cell (Montironi et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

et al. 2020

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Abstract. Haichour R, Lograda T, Ramdani M, Chalard P, Figueredo G. 2020. Chemical composition and antimicrobial activity of Pinus halepensis from Algeria. Biodiversitas 21: 4345-4360. The chemical composition of Pinus halepensis essential oils and their antibacterial activities were investigated. Plant samples were collected in the flowering stage from the East locality of Algeria. The aerial parts of P. halepensis obtained from fifteen localities were subjected to a hydro-distillation, providing pale yellow viscous oils. The obtained yield average was 0.64 ± 0.37%. The analysis of the chemical composition of essential oils was performed by using GC and GC / MS. A total of thirty-seven compounds representing 99.4 ± 0.5% of the total oils were identified in P. halepensis. The hydrocarbon compounds were found to dominate essential oils in P. halepensis. In addition, the major compounds were caryophyllene-E (29.06 ± 8.5%), myrcene (19.14 ± 6.67%), a-pinene (16.86 ± 3.35%), phenyl ethyl 3-methyl butanoate (5.67 ± 2.47%), a-humulene (4.81 ± 1.43%), terpinolene (3.94 ± 1.6%) and sabinene (3.11 ± 1.84%). Based on their chemical composition, essential oil samples were clustered in five groups by UPGMA analysis. The first group includes the Tafrent essential oil population. It is characterized by the phenyl ethyl-3-methyl butanoate – Sabinene ‒ Δ3-carene chemotype. The second group was subdivided into two clusters. The first cluster or chemotype was characterized by the myrcene ‒ α-pinene ‒ caryophyllene-E. The second chemotype was characterized by caryophyllene-E ‒ α-humulene ‒ myrcene. The essential oil of P. halepensis is an effective antimicrobial against Staphylococcus aureus; Klebsiella pneumonia; Salmonella enteric spp arizonae; Listeria innocua; Proteus mirabilis and Bacillus cereus, and no effect on the E. coli and Pseudomonas aeruginosa.

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Section: Discussionmentioning

confidence: 99%

Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

et al. 2020

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“…Some researchers show that pinus oil have an antimicrobial activity. The essensial pinus inhibited Staphylococcus aureus growth (Fit et al, 2009 ;Joshua et al, 2014;Leandro et al, 2014;Raho, 2014), Escherichia coli (Raho, 2014) Up to now, the information concerning the activity of Chloroxylenol and pine oil against…”

Section: Chloroxylenol Is Also Known As P-chloro-mentioning

confidence: 99%

Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant

et al. 2020

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Vibrio spp. genus is known as a marine indigeneous bacteria. Vibrio parahaemolyticus, V. vulnificus and V. harveyi are pathogenic Vibrio. This study aims to assess the sensitivity of three Vibrio species (V parahaemolyticus, V. vulnificus and V. harveyi) isolated from shrimp pond against two type of disinfectant with different active compound namely Chloroxylenol (4-Chloro-3,5-dimethylphenol, C8H9ClO) and pine oil. The assessment was done by Kirby-Bauer disk diffusion methods in Zobell agar media with two different concentration (10 and 100 ppm) and replicated in three times. Sensitivity of Vibrio spp. was analized based on the inhibition zone activity produced by disinfectant. Results showed that sensitivity of Vibrio spp. against disinfectant Chloroxylenol 4.8% at 100 ppm were higher than 10 ppm. The increment of V parahaemolyticus was 182 %, V. vulnificus was 47 % and V. harveyi was 43 %, respectively. Susceptibility of antiseptic with Chloroxylenol 4.8% at 100 ppm was arised to 152 % (V. parahaemolyticus), 43 % (V. vulnificus) and 31 % (V. harveyi) when compared to 2.5% pine oil disinfectant. It can be concluded that Chloroxylenol 4,8 % active compound and pine oil were able to inhibit the Vibrio spp. growth.

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“…have been used to treat wounds, respiratory ailments, and skin infections. − Moreover, various therapeutic properties have been identified for EO from P. halepensis, such as anticoagulant, anti-inflammatory, − antimicrobial, , antibacterial, ,, antifungal, − larvicidal, antioxidant, ,,, and anticancer. , …”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Essential Oils from Tunisian Pinus Halepensis Mill. by Hydrodistillation and Microwave-Assisted Processes: Chemical Composition and Antioxidant and Cytotoxic Potential against Prostate and Cervical Cancer Cells

Hamzaoui,

Zallez,

Buñay

et al. 2024

ACS Omega

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Pinus halepensis Mill. is a Mediterranean aromatic plant largely used, in addition to its nutritional value, in traditional medicine as antiseptic, antifungal, antituberculotic, and antirheumatic. Thus, the objective of this work was to appraise the antioxidant and cytotoxic activity of the essential oil (EO) of P. halepensis from Tunisia on cancer cell cultures, along with chemical composition evaluation by GC−MS. To attain the best yield and also highest quality in extraction of the EOs, conventional hydrodistillation (HD) and novel microwave-assisted extraction (MAE) methods have been performed and compared. The antioxidant activity was evaluated through the inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH)• radicals. The cytotoxic activity in prostate cancer cells (LNCaP and PC3) and cervical cancer cells (HeLa) of EO was evaluated by the MTT assay and effect on the cell cycle by flow cytometry analysis. A total of 38 and 37 components were identified from HD (HD-EO)-and MAE (MAE-EO)extracted EOs, respectively, which were dominated by hydrocarbon compounds (HD-EO = 86.65%; MAE-EO = 77.36%), especially monoterpenes (HD-EO = 32.11%; MAE-EO = 21.55%) and sesquiterpenes (HD-EO = 44.29%; MAE-EO = 61.32%). Both extracted EOs showed significant antioxidant activity, as shown by the inhibition of DPPH• radicals [IC 50 (HD-EO) = 4102.30 ± 159.73 μg mL −1 and IC 50 (MAE-EO) = 3430.13 ± 78.46 μg mL −1 ]. Also, the EOs exhibited substantial (p < 0.001) antiproliferative activities with G0-G1 arrest on PC3, LNCaP, and HeLa cells by yielding very low IC 50 values more conspicuous in MAE-EO with respective IC 50 values of 25.70 ± 6.58, 14.97 ± 3.21, and 14.55 ± 2.30 μg mL −1 . This finding points out for the first time that the EO of P. halepensis Mill. from Tunisia can be an effective natural antitumor agent with more pronounced activity when extracted with the MAE method that, after further in vivo studies, can be harnessed as a putative phytopharmaceutical for prostate and cervical cancer treatment.

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Antibacterial potential of essential oils of the needles of Pinus halepensis against Staphylococcus aureus and Escherichia coli

Cited by 4 publications

References 18 publications

Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

Chemical composition and antimicrobial activity of Pinus halepensis from Algeria

Sensitivity of Vibrio parahaemolyticus, V. vulnificus and V. harveyi Against Chloroxylenol (4-Chloro-3,5-dimethylphenol , C8H9ClO) Antiseptic and Pine Oil Disinfectant

Comparative Study of Essential Oils from Tunisian Pinus Halepensis Mill. by Hydrodistillation and Microwave-Assisted Processes: Chemical Composition and Antioxidant and Cytotoxic Potential against Prostate and Cervical Cancer Cells

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