Antibacterial potential of a novel<i>Lactobacillus casei</i>strain isolated from Chinese northeast sauerkraut and the antibiofilm activity of its exopolysaccharides

Xu, Xiaoqing; Peng, Qing; Zhang, Yuwei; Tian, Dandan; Zhang, Pengbo; Ying, Huang; Ma, Lan; Dia, Vermont P.; Qiao, Yu; Shi, Bo

doi:10.1039/d0fo00905a

Cited by 43 publications

(23 citation statements)

References 59 publications

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“…The beneficial functions may be related to secretory products especially exopolysaccharides (EPS) [10]. Bacterial EPS are partly secreted into the environment as secondary metabolic products and partly covered the bacterial surface [11] and can be classified into homopolysaccharides and heteropolysaccharides according to the monomer composition and mechanisms of biosynthesis [12]. The reported EPS from Bifidobacterium are all heteropolysaccharides composed of galactose, glucose, and rhamnose, among others [13].…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy

et al. 2021

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Enteropathogenic Escherichia coli (EPEC) is a common zoonotic pathogen that causes acute infectious diarrhea. Probiotics like Bifidobacterium are known to help prevent pathogen infections. The protective effects of Bifidobacterium are closely associated with its secretory products exopolysaccharides (EPS). We explored the effects of the EPS from Bifidobacterium animalis subsp. lactis (B. lactis) on ameliorating the damage of an intestinal porcine epithelial cell line (IPEC-J2) during EPEC infection. Pretreatment with EPS alleviated EPEC-induced apoptosis through the restoration of cell morphology and the downregulation of protein expressions of cleaved-caspase 8, cleaved-caspase 3, and cleaved-PARP. EPS-mediated remission of apoptosis significantly improved cell viability during EPEC infection. EPEC infection also resulted in impaired autophagy, as demonstrated by decreased expressions of autophagy-related proteins Beclin 1, ATG5, and microtubule-binding protein light chain-3B (LC3B) and the increased expression of p62 through western blot analysis. However, EPS reversed these effects which indicated that EPS promoted autophagosome formation. Furthermore, EPS prevented the lysosome damage induced by EPEC as it enhanced lysosomal acidification and raised lysosome-associated protein levels, thus promoted autophagosome degradation. Our findings suggest that the amelioration of EPEC-induced cell damages by EPS is associated with the limitation of detrimental apoptosis and the promotion of autophagy flux.

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Section: Introductionmentioning

confidence: 99%

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy

et al. 2021

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“…L. plantarum with excellent probiotic properties had been proved to relieve diarrhea caused by enterotoxigenic E. coli (ETEC) ( 17 ). Xu et al ( 18 ) reported the antibacterial activity of L. casei NA-2 against Bacillus cereus, S. aureus, S. typhimurium , and E. coli O157:H7, together with its potential antibacterial properties of exopolysaccharide (EPS). An increasing data has shown the EPS from Lactobacillus exerts antibacterial activities by inhibiting biofilm formation and dispersing of various bacteria, such as S. aureus ( 19 ), E. coli ( 16 ), Listeria monocytogenes, S. typhimurium , and P. aeruginosa ( 20 ).…”

Section: Discussionmentioning

confidence: 99%

The Probiotic Attributes and Anti-pseudorabies Virus Evaluation of Lactobacillus Isolates

Yang

Yan

et al. 2022

Front. Vet. Sci.

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The emergence of pseudorabies virus (PRV) variants brings serious harm to the swine industry, and its effective treatments are limited at present. As one of the probiotics, the Lactobacillus species have beneficial characteristics of regulating the balance of intestinal flora, inhibiting the growth of pathogenic bacteria and viruses' proliferation, and improving self-immunity. In this study, Lactobacillus plantarum HN-11 and Lactobacillus casei HN-12 were selected and identified through morphology observation, Gram stain microscopy, 16S rRNA sequencing analysis, and specific amplification of the recA gene and pheS gene. All tested isolates exhibited rapid adaptation to the different conditions, excellent acid, and bile tolerance, and sensitivity to Salmonella, Staphylococcus aureus, and Escherichia coli. The antibiotic susceptibility assay displayed the isolates sensitive to most antibiotics and resistant to Lincomycin and Norfloxacin. Moreover, the supernatants of HN-11 and HN-12 inhibited PRV proliferation in ST cells. The results of animal experiments showed that supplementing the challenged mice with the supernatants of Lactobacillus isolates in advance delayed the course of the disease. PRV was detected in the heart, liver, spleen, lung, kidney, and brain tissues of dead mice in the test groups, and its copies in the lungs were significantly decreased compared with the control mice (P < 0.05). These findings proved the advantages of L. plantarum and L. casei as potential probiotic cultures, which could provide a basis for its application in microecological preparations and functional formulations.

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“…For instance, EPSs isolated from Enterococcus faecium MC13 inhibited biofilm formation in L. monocytogenes ( 124 ). Additionally, an EPSs producing Lactobacillus casei probiotic strain when co-cultured with various pathogenic bacteria, inhibited the growth of B. cereus, S. aureus, S. typhimurium and E. coli O157:H7 ( 125 ).…”

Section: Prebiotic Properties Of Microbial Polysaccharidesmentioning

confidence: 99%

Bacterial polysaccharides—A big source for prebiotics and therapeutics

Khan

Shah

et al. 2022

Front. Nutr.

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Bacterial polysaccharides are unique due to their higher purity, hydrophilic nature, and a finer three-dimensional fibrous structure. Primarily, these polymers provide protection, support, and energy to the microorganism, however, more recently several auxiliary properties of these biopolymers have been unmasked. Microbial polysaccharides have shown therapeutic abilities against various illnesses, augmented the healing abilities of the herbal and Western medicines, improved overall health of the host, and have exerted positive impact on the growth of gut dwelling beneficial bacteria. Specifically, the review is discussing the mechanism through which bacterial polysaccharides exert anti-inflammatory, antioxidant, anti-cancer, and anti-microbial properties. In addition, they are holding promising application in the 3D printing. The review is also discussing a perspective about the metagenome-based screening of polysaccharides, their integration with other cutting-edge tools, and synthetic microbiome base intervention of polysaccharides as a strategy for prebiotic intervention. This review has collected interesting information about the bacterial polysaccharides from Google Scholar, PubMed, Scopus, and Web of Science databases. Up to our knowledge, this is the first of its kind review article that is summarizing therapeutic, prebiotics, and commercial application of bacterial polysaccharides.

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Antibacterial potential of a novelLactobacillus caseistrain isolated from Chinese northeast sauerkraut and the antibiofilm activity of its exopolysaccharides

Abstract: Exopolysaccharide-producing Lactobacillus spp. isolated from sauerkraut, as probiotics, have shown efficacy in the inhibition of pathogenic bacteria in the intestinal tract.

Cited by 43 publications

References 59 publications

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy

Exopolysaccharides from Bifidobacterium animalis Ameliorate Escherichia coli-Induced IPEC-J2 Cell Damage via Inhibiting Apoptosis and Restoring Autophagy

The Probiotic Attributes and Anti-pseudorabies Virus Evaluation of Lactobacillus Isolates

Bacterial polysaccharides—A big source for prebiotics and therapeutics

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