Antibacterial potency of garlic extract against certain skin pathogenic bacteria

Abstract

The antibacterial potency of garlic extract (Allium sativum) against gram positive and gram negative skin pathogenic bacteria including; Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, and Pseudomonas aeruginosa were studied using agar well diffusion and broth dilution assays.Agar well diffusion assay for aqueous garlic extract (AGE) was characterized with zones of inhibition ranging from 4.40 -3.80cm, 4.13 -3.57cm, 3.40 -2.67cm for S. aureus, S. epidermidis and Strep. pyogenes, respectively, however, Ps. aeruginosa had lesser zone of inhibition ranging from 2.32 -1.55cm. Studying the antibacterial potency of AGE against the selected isolates, revealed that it is affected by temperature of storage. Current results showed that storage of AGE at low temperature of -20 o C, does not affect its potency, however, its potency was slightly lost at high temperatures above 37 o C. The broth dilution test was performed to investigate the Minimum inhibitory concentration (MIC) andMinimum bactericidal concentration (MBC) of the AGE against the bacterial isolates at 37 o C.Investigating the activity of AGE loaded on Gel dressing revealed that it can have potency when applied on patients with Staphylococcal skin infections. Findings from this study encourage and support the use of AGE in treating bacterial skin infections especially in developing countries like Africa, as it is available, economic and have no side effects.

Phenotypic Analysis, Molecular Characterization, and Antibiogram of Caries-Causing Bacteria Isolated from Dental Patients

“…pyogenes with diameter zones of inhibition ranging from 3.40 cm to 2.67 cm. P. aeruginosa was found to have the smallest inhibitory zones, which were 2.32 cm–1.55 cm in diameter [ 115 ]. A 31 mm inhibitory zone has been observed for both fresh local and imported garlic extracts when used against methicillin-resistant S. aureus (MRSA).…”

Anti-bacterial and Anti-fungal Properties of Garlic Extract (Allium sativum): A Review

“…pyogenes with zones of inhibition ranging from 3.40 -2.67 cm. The least inhibition zones recorded about 2.32 -1.55 cm were observed in P. aeruginosa [15]. Fresh domestic and imported garlic extract are known to play bacteriostatic activity against the methicillin-resistant Staphylococcus aureus (MRSA) with 31mm inhibition zone [16].…”

Antimicrobial Activities of Plant Extracts against Solanum tuberosum L. Phytopathogens

“…The antibacterial property of garlic extracts against several human pathogens, including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella spp., and Streptococcus mutans has already been described [ 28 , 29 , 30 , 31 , 32 ]. Although the activity of garlic extracts against potato phytopathogens is under-reported, a garlic WE was active against Alternaria solani [ 33 ], while chloroform, hexane, methanol [ 34 ], and water [ 22 ] extracts inhibited the growth of Rhizoctonia solani .…”

“…Previous studies reported inhibition of A. alternata growth by peppermint and lavender methanol-water extract [10] and peppermint essential oil [26]; however, due to a different type of extract, these findings were not reflected in the present work. The detailed results of phytopathogen growth inhibition by water-glycol extracts The antibacterial property of garlic extracts against several human pathogens, including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella spp., and Streptococcus mutans has already been described [28][29][30][31][32]. Although the activity of garlic extracts against potato phytopathogens is under-reported, a garlic WE was active against Alternaria solani [33], while chloroform, hexane, methanol [34], and water [22] extracts inhibited the growth of Rhizoctonia solani.…”