Antibacterial photodynamic therapy for dental caries: Evaluation of the photosensitizers used and light source properties

Nagata, Juliana Yuri; Hioka, Noboru; Kimura, Elza; Batistela, Vagner Roberto; Terada, Raquel Sano Suga; Graciano, Ariane Ximenes; Baesso, Mauro Luciano; Fujimaki, Mitsue

doi:10.1016/j.pdpdt.2011.11.006

Cited by 176 publications

(109 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…3 Percentage of surviving fractions of S. mutans and L. acidophilus grown as multispecies in dentine carious lesions according to curcumin concentrations. Same letter (A) means no significant difference among the groups (p >0.05) practice [35]. In this study, curcumin was appropriately activated by an LED source (450 nm).…”

Section: Discussionmentioning

confidence: 77%

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

et al. 2013

View full text Add to dashboard Cite

Photodynamic therapy (PDT) is a technique that involves the activation of photosensitizers by light in the presence of oxygen, resulting in the production of reactive radicals that are capable of inducing cell death. The present study evaluated the susceptibility of Streptococcus mutans and Lactobacillus acidophilus to PDT grown as multi-species in the biofilm phase versus in dentine carious lesions. A brainheart infusion culture medium supplemented with 1 % glucose, 2 % sucrose, and 1 % young primary culture of L. acidophilus 10 8 CFU/mL and S. mutans 10 8 CFU/mL was used to develop multi-species biofilms and to induce caries on human dentine slabs. Five different concentrations of curcumin (0.75, 1.5, 3.0, 4.0, and 5.0 g/L) were used associated with 5.7 J/cm 2 light emission diode. Four different groups were analyzed L−D− (control group), L−D+ (drug group), L+D− (light group), and L+D+ (PDT group). ANOVA/Tukey's tests were conducted to compare groups. A significant reduction (p <0.05) in cell viability was observed in the biofilm phase following photosensitization with all curcumin concentrations tested. To achieve significant bacterial reduction (p <0.05) in carious dentine, it was necessary to utilize 5.0 g/L of curcumin in association with blue light. No significant reduction was found for L−D+, supporting the absence of the drug's dark toxicity. S. mutans and L. acidophilus were susceptible to curcumin in the presence of blue light. However, due to light penetration and drug diffusion difficulties, these microorganisms within dentine carious lesions were less affected than they were in the biofilm phase.

show abstract

Section: Discussionmentioning

confidence: 77%

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

et al. 2013

View full text Add to dashboard Cite

show abstract

“…It has been used widely in the biological arena as a stain in the pathology of both cancer and microbial disease [1,2]. More recently TBO has been widely examined as a photoantimicrobial agent aimed at oral disinfection [3,4], and is a lead compound in azine photosensitiser research and development [5]. Where comparative testing of the photoantimicrobial efficacies of MB and TBO have been carried out, little overall difference has been reported [6].…”

Section: Introductionmentioning

confidence: 99%

Phenothiazinium photosensitisers XI. Improved toluidine blue photoantimicrobials

Wainwright

O'Kane

Rawthore

2016

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

The phenothiazinium derivative toluidine blue O (TBO) is widely employed as a photoantimicrobial agent in clinical trialling, particularly in dentistry. However, its activity against a range of pathogenic microbial species is not significantly different to that of the standard photoantimicrobial methylene blue. In the current study, derivatives of TBO with varying hydrocarbon substitution in chromophore position 2 were synthesised via the established anilinethiosulphonic route, using the mild oxidant silver(II) carbonate to allow substituent preservation.The resulting series of analogues demonstrated the expected increases in visible absorption wavelength and lipophilicity with increasing hydrocarbon content, as well as decreased aggregation for derivatives with bulkier substituents, and all produced singlet oxygen on illumination in vitro. Screening against a range of bacterial and fungal pathogens relevant to infection control showed remarkable increases in activity relative to the parent compound, particularly against the clinically important Gram-negative bacterium Pseudomonas aeruginosa. In addition, in order to demonstrate clinical relevance, the photoactivities of the new derivatives against microbial targets were compared to conventional antibacterial and antifungal drugs, as well as biocides commonly used for local disinfection. Activity here was also generally greater than that of the conventional agents used for comparison, considerably so relative to the local disinfectant agents.

show abstract

“…30 Photodynamic therapy has also been used to disinfect caries dentin before restoration, disinfecting oral tissues before or during surgical procedures, treating denture stomatitis, and treating oral candidiasis in immunocompromised patients. 31,32 With regard to bacteria involved in dental caries, Burns et al 33 showed that tolonium chloride (25 g/mL)…”

Section: Photodynamic Therapy In Dentistrymentioning

confidence: 99%

Photodynamic Therapy in Endodontics

Mohammadi¹,

Jafarzadeh²,

Shalavi³

et al. 2017

The Journal of Contemporary Dental Practice

View full text Add to dashboard Cite

Commonly used irrigants do not always eradicate the entire microbial flora in infected root canals. Therefore, several other strategies, such as photodynamic therapy (PDT) have been developed. Photoactivated disinfection is based on the interaction of a photosensitive antibacterial agent and a light source. It uses a nontoxic dye named photosensitizer (PS) and lowintensity visible light. In oxygen presentation, these combine to produce some cytotoxic species. The PS molecules attach to bacteria membrane. Irradiation with a specific wavelength of the light may lead to the production of singlet oxygen, resulting in rupture of the microbial cell wall. There are several applications for PDT in dentistry. A successful periodontal treatment is based on elimination of bacteria from the infected area. Phenothiazinium PSs have been shown to be highly effective and safe for this purpose. However, scaling/root planing should be performed before the PDT. While performing the PDT, PS should be first injected in the periodontal pocket and allowed to pigment. Then, the special fiber should be inserted 1 mm short of the pocket base and lased. Photodynamic therapy has also been used to disinfect caries dentin before restoration, disinfecting oral tissues before or during surgical procedures, treating denture stomatitis, and treating oral candidiasis in immunocompromised patients. Photodynamic therapy can be used in combination with mechanical instrumentation and chemical antimicrobial agents, such as sodium hypochlorite, too. The purpose of this study was to review historical perspective, mechanism of action, and applications of PDT in dentistry and especially in endodontics was reviewed. Furthermore, the effects of PDT on dentin bonding and endotoxin are discussed.Clinical significance: Photodynamic therapy has been advocated to increase the disinfection level of the root canal system.

show abstract

Antibacterial photodynamic therapy for dental caries: Evaluation of the photosensitizers used and light source properties

Cited by 176 publications

References 50 publications

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

Photodynamic antimicrobial therapy of curcumin in biofilms and carious dentine

Phenothiazinium photosensitisers XI. Improved toluidine blue photoantimicrobials

Photodynamic Therapy in Endodontics

Contact Info

Product

Resources

About