Antibacterial Peptides

Sabatier, Jean‐Marc

doi:10.3390/antibiotics9040142

Cited by 4 publications

(2 citation statements)

References 10 publications

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“…In addition to bactericidal metal ions, AMPs have emerged as promising antibacterial agents due to their amphipathic character, which enables their interaction with and subsequent disruption of bacterial membranes [ 63 – 65 ]. However, the clinical translation of AMPs was limited by their limited bioavailability, susceptibility to enzymatic degradation, and low penetrability toward the target infections [ 66 , 67 ]. Thus, efficient delivery methods are required to help these molecules reach their target area.…”

Section: Antibacterial Strategy Based On Mnmsmentioning

confidence: 99%

Antibacterial micro/nanomotors: advancing biofilm research to support medical applications

Jiang,

Fu,

Wei

et al. 2023

J Nanobiotechnol

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Multi-drug resistant (MDR) bacterial infections are gradually increasing in the global scope, causing a serious burden to patients and society. The formation of bacterial biofilms, which is one of the key reasons for antibiotic resistance, blocks antibiotic penetration by forming a physical barrier. Nano/micro motors (MNMs) are micro-/nanoscale devices capable of performing complex tasks in the bacterial microenvironment by transforming various energy sources (including chemical fuels or external physical fields) into mechanical motion or actuation. This autonomous movement provides significant advantages in breaking through biological barriers and accelerating drug diffusion. In recent years, MNMs with high penetrating power have been used as carriers of antibiotics to overcome bacterial biofilms, enabling efficient drug delivery and improving the therapeutic effectiveness of MDR bacterial infections. Additionally, non-antibiotic antibacterial strategies based on nanomaterials, such as photothermal therapy and photodynamic therapy, are continuously being developed due to their non-invasive nature, high effectiveness, and non-induction of resistance. Therefore, multifunctional MNMs have broad prospects in the treatment of MDR bacterial infections. This review discusses the performance of MNMs in the breakthrough and elimination of bacterial biofilms, as well as their application in the field of anti-infection. Finally, the challenges and future development directions of antibacterial MNMs are introduced.

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Section: Antibacterial Strategy Based On Mnmsmentioning

confidence: 99%

Antibacterial micro/nanomotors: advancing biofilm research to support medical applications

Jiang,

Fu,

Wei

et al. 2023

J Nanobiotechnol

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“…Antimicrobial peptides (AMPs), a diverse family of short peptides that are important for plants and animals, are powerful weapons in host defense mechanisms and are present in all life domains [ 1 ]. Defensins, which are widely generated by fungi, insects, and vertebrates, are endogenous cationic AMPs that act as the main effectors of the innate immune system, owing to their broad-spectrum antimicrobial activities [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Properties and Mode of Action of Cryptdin-4, a Mouse α-Defensin Regulated by Peptide Redox Structures and Bacterial Cultivation Conditions

et al. 2023

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Cryptdin-4 (crp4) is an enteric α-defensin derived from mice, and is a main mediator of immunity to oral infections and a determinant of the composition of the intestinal microbiota. Structurally, crp4 exists in two states: the oxidized form (crp4oxi), constrained by three invariant disulfide bonds, and the reduced form (crp4red) with six free thiol groups, both of which exist in the intestinal tract. In this study, the antibacterial mechanisms of crp4 in both forms under aerobic and anaerobic conditions were investigated using Escherichia coli (E. coli), an anaerobic facultative bacterium, as a model. Fluorescent dye studies revealed that both crp4oxi and crp4red exhibited antimicrobial activity against cells cultured under aerobic conditions via rapid membrane depolarization. Furthermore, the antioxidant treatment experiments suggested that only crp4oxi exhibited antimicrobial activity by the induction and accumulation of reactive oxygen species (ROS). However, under anaerobic culture conditions, the ability of both forms to disrupt the function of bacterial membranes decreased and activity was greatly reduced, but crp4red maintained some antimicrobial activity. This activity may be due to the inhibition of intracellular functions by DNA binding. Altogether, these data indicate that, according to its redox structure and the environmental redox conditions, crp4 could perform different antimicrobial activities via different mechanisms.

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A Novel In silico Filtration Method for Discovery of Encrypted Antimicrobial Peptides

Barneh,

Nazarian,

Mousavi Nadoshan

et al. 2024

CBIO

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Background:: Antibacterial resistance has been one of the most important causes of death in the last few decades, necessitating the need to discover new antibiotics. Antimicrobial peptides (AMPs) are among the best candidates due to their broad-spectrum and potent activity against bacteria and low probability of developing resistance against them. Objective:: In this study, we proposed a novel filtration method using knowledge-based approaches to discover encrypted AMPs within a protein sequence Methods:: The encrypted AMPs were selected from a protein sequence, in this case, lactoferrin, based on hydrophobicity, cationicity, alpha-helix structure, helical wheel projection, and binding affinities to gram-negative and positive bacterial membranes. Results:: Six out of 20 potential encrypted AMPs were ultimately selected for further assays. Molecular docking of the selected AMPs with outer and inner membranes of gram-negative bacteria and also gram-positive bacterial membranes showed reasonable binding affinity ranging from ‘-6.7 to -7.5’ and ‘- 4.5 to -5.7’ and ‘-4.6 to -5.7’ kcal/mol, respectively. No toxicity was shown in the candidate AMPs. Conclusion:: According to in silico results, our method succeeded to discover six new encrypted AMPs from human lactoferrin, designated as lactoferrin-derived peptides (LDPs). Further in silico and experimental assays should also be performed to prove the efficiency of our knowledge-based filtration method.

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Antibacterial Peptides

Cited by 4 publications

References 10 publications

Antibacterial micro/nanomotors: advancing biofilm research to support medical applications

Antibacterial micro/nanomotors: advancing biofilm research to support medical applications

Antimicrobial Properties and Mode of Action of Cryptdin-4, a Mouse α-Defensin Regulated by Peptide Redox Structures and Bacterial Cultivation Conditions

A Novel In silico Filtration Method for Discovery of Encrypted Antimicrobial Peptides

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