Antibacterial Mechanism of Silkworm Seroins

Zhu, Hongtao; Zhang, Xiaolu; Lu, Meng‐Yao; Chen, Haiqin; Chen, Shiyi; Han, Jiaxuan; Zhang, Yan; Zhao, Ping; Dong, Zhaoming

doi:10.3390/polym12122985

Cited by 17 publications

(9 citation statements)

References 32 publications

(42 reference statements)

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“…Noticeably, the SF/FG composites also exhibit moderate antibacterial activity, with a bacterial inactivation rate of 9.63%, which can be attributed to the antimicrobial activity of the abundant seroin in silk. [38] The above results reveal that the synergetic antibacterial activity of ZnO NPs and SF microspheres is what endows the ZSF/FG composites with remarkable antibacterial efficacy.…”

Section: Resultsmentioning

confidence: 75%

“…However, the direct incorporation of ZnO NPs into FG achieved an undesirable lethality (less than 85.2%), as shown in Figure S6, which may be attributed to the antioxidant activity of FG. Noticeably, the SF/FG composites also exhibit moderate antibacterial activity, with a bacterial inactivation rate of 9.63%, which can be attributed to the antimicrobial activity of the abundant seroin in silk [38] . The above results reveal that the synergetic antibacterial activity of ZnO NPs and SF microspheres is what endows the ZSF/FG composites with remarkable antibacterial efficacy.…”

Section: Resultsmentioning

confidence: 76%

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Biomass‐derived washable composites for accelerating the healing of infected wounds

Jiao,

Zhao,

Zhao

et al. 2023

BMEMat

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Advanced sustainable biomedical materials are urgently needed for clinical applications; however, developing biomedical materials with exceptional mechanical and bactericidal properties as well as removable functionalities to reduce unintended secondary injury remains a challenge. Here, we report a biomass‐derived composite consisting of water‐soluble fish gelatin (FG) and antibacterial ZnO@silk fibroin (ZSF) microspheres for potential application as the wound dressing. The ZSF microspheres are embedded in a FG matrix to realize the stretchable, antibacterial, and removable ZSF/FG composites. By introducing glycerin as the plasticizer, ZSF/FG composites deliver a tensile strength of 4.5 MPa and stretchability of 550%. Acting as both the germicide and hydrophile components, ZSF microspheres endow the composites with excellent antibacterial capacity and water solubility. To prevent secondary injury, the ZSF/FG composites can be easily removed from the wounds by simply exposing them to excess water. Additionally, the ZSF/FG composites exhibit favorable biocompatibility and sustain high cell viability of over 100%. The full‐thickness skin wound model on infected mice demonstrated an efficient rate of wound closure and a reduced inflammatory response. The ZSF/FG composite shows promise to hasten the healing of infected wounds and is expected a promising candidate as wound dressing for clinical therapy.

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Section: Resultsmentioning

confidence: 75%

Section: Resultsmentioning

confidence: 76%

Biomass‐derived washable composites for accelerating the healing of infected wounds

Jiao,

Zhao,

Zhao

et al. 2023

BMEMat

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“…While the antimicrobial properties of seroin 1 and 2 are well known, seroin 3 has only recently been studied. A recent study showed that seroin 1, seroin 1-N, and seroin 1-C had no bacteriostatic effect on Escherichia coli , seroin 2 had better antibacterial activity, and seroin 3 had stronger bacteriostatic activity against Gram-positive bacteria than against Gram-negative strains ( Zhu et al, 2020 ).…”

Section: Natural Products With Antibacterial Activitymentioning

confidence: 99%

Natural Compounds With Antimicrobial and Antiviral Effect and Nanocarriers Used for Their Transportation

Stan

Enciu

Mateescu³

et al. 2021

Front. Pharmacol.

104

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Due to the increasing prevalence of life-threatening bacterial, fungal and viral infections and the ability of these human pathogens to develop resistance to current treatment strategies, there is a great need to find and develop new compunds to combat them. These molecules must have low toxicity, specific activity and high bioavailability. The most suitable compounds for this task are usually derived from natural sources (animal, plant or even microbial). In this review article, the latest and most promising natural compounds used to combat bacteria, filamentous fungi and viruses are presented and evaluated. These include plant extracts, essential oils, small antimicrobial peptides of animal origin, bacteriocins and various groups of plant compounds (triterpenoids; alkaloids; phenols; flavonoids) with antimicrobial and antiviral activity. Data are presented on the inhibitory activity of each natural antimicrobial substance and on the putative mechanism of action against bacterial and fungal strains. The results show that among the bioactive compounds studied, triterpenoids have significant inhibitory activity against coronaviruses, but flavonoids have also been shown to inhibit SARS-COV-2. The last chapter is devoted to nanocarriers used to improve stability, bioavailability, cellular uptake/internalization, pharmacokinetic profile and reduce toxicity of natural compunds. There are a number of nanocarriers such as liposomes, drug delivery microemulsion systems, nanocapsules, solid lipid nanoparticles, polymeric micelles, dendrimers, etc. However, some of the recent studies have focused on the incorporation of natural substances with antimicrobial/antiviral activity into polymeric nanoparticles, niosomes and silver nanoparticles (which have been shown to have intrinsic antimicrobial activity). The natural antimicrobials isolated from animals and microorganisms have been shown to have good inhibitory effect on a range of pathogens, however the plants remain the most prolific source. Even if the majority of the studies for the biological activity evaluation are in silico or in vitro, their internalization in the optimum nanocarriers represents the future of “green therapeutics” as shown by some of the recent work in the field.

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“…First, the cocoons, which are immobile and non-metabolizing entities, must be protected from infection and ensuing decay. This explains the presence in their composition of defense antimicrobial proteins [ 98 , 99 , 100 , 101 ]. Second, the cocoons are themselves responsible for the survival of the species by protecting the pupae from pathogens, predators, sunlight, humidity, and heat.…”

Section: Discussionmentioning

confidence: 99%

“…The composition of non-protein matter in B. mori silk cocoon is traditionally reported [ 103 , 104 ] as 1.2–1.6% carbohydrates, ∼0.7% inorganic matter, 0.4–0.8% wax matter, and ∼0.2% pigments. The rest consists of the two major proteins in the silk thread, fibroin, and sericin, which are accompanied by much lower amounts of other proteins having either identifiable defense functions (enzymes, seroins, protease inhibitors) or other roles yet to be determined [ 99 , 100 , 101 , 104 ]. Regarding BMSS itself, the number and the distribution of constituting peptidoid units are still disputed.…”

Section: Discussionmentioning

confidence: 99%

Study of the Antioxidative Effects of Bombyx mori Silk Sericin in Cultures of Murine Retinal Photoreceptor Cells

2022

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The availability of natural substances able to fulfill the role of antioxidants in a physiologic environment is important for the development of therapies against diseases associated with excessive production of reactive oxygen species and ensuing oxidative stress. Antioxidant properties have been reported episodically for sericin, a proteinaceous constituent of the silk thread in the cocoons generated by the larvae of the Lepidoptera order. We investigated the sericin fractions isolated from the cocoons spun by the domesticated (Bombyx mori) silkworm. Three fractions were isolated and evaluated, including two peptidoid fractions, the crude sericin and the purified (dialyzed) sericin, and the non-peptidoid methanolic extract of the crude fraction. When subjected to Trolox equivalent antioxidant capacity (TEAC) assay, the extract showed much higher antioxidant capacity as compared to the crude or purified sericin fractions. The three fractions were also evaluated in cultures of murine retinal photoreceptor cells (661 W), a cell line that is highly susceptible to oxidants and is crucially involved in the retinopathies primarily caused by oxidative stress. The extract displayed a significant dose-dependent protective effect on the cultured cells exposed to hydrogen peroxide. In identical conditions, the crude sericin showed a certain level of antioxidative activity at a higher concentration, while the purified sericin did not show any activity. We concluded that the non-peptidoid components accompanying sericin were chiefly responsible for the previously reported antioxidant capacity associated with sericin fractions, a conclusion supported by the qualitative detection of flavonoids in the extract but not in the purified sericin fraction.

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Antibacterial Mechanism of Silkworm Seroins

Cited by 17 publications

References 32 publications

Biomass‐derived washable composites for accelerating the healing of infected wounds

Biomass‐derived washable composites for accelerating the healing of infected wounds

Natural Compounds With Antimicrobial and Antiviral Effect and Nanocarriers Used for Their Transportation

Study of the Antioxidative Effects of Bombyx mori Silk Sericin in Cultures of Murine Retinal Photoreceptor Cells

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