Antibacterial labdane diterpenoids of Ulva fasciata Delile from southwestern coast of the Indian Peninsula

Chakraborty, Kajal; Lipton, A P; Raj, Rashmi; Vijayan, Koyadan Kizhakedath

doi:10.1016/j.foodchem.2009.09.019

Cited by 90 publications

(39 citation statements)

References 26 publications

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“…Other bioactive algal metabolites isolated from non-polar extracts include cytotoxic, antibacterial linear sesquiterpenoids from the green alga Penicillus capitatus (class Ulvophyceae, order Bryopsidales) (Paul and Fenical 1984); antibacterial labdane diterpenoids from Ulva fasciata (Chakraborty et al 2010); and antiatherosclerotic phytosterols from the brown alga Sargassum fusiforme (order Fucules) (Chen et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity

et al. 2014

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Increasingly, macroalgae are being recognised as a growth opportunity for functional foods and nutritional security in the future. Dominating traits of interest are metabolites that function as anti-inflammatories and are antiproliferative. However, seaweeds from the northern hemisphere dominate this field of research. Australia has a unique flora of macroalgae, and it is poorly understood which species should be targeted for cultivation towards food and health markets. Here, six Australian marine macroalgae were selected for screening of one anti-inflammatory group; n-3 polyunsaturated fatty acids (PUFA). PUFA profiles were determined using gas chromatography-mass spectrometry and multivariate analysis. Thirty-one fatty acids (FA) were identified across the six macroalgal species with C16:0 the dominant FA in all samples, variations across taxa in the saturated FA C10:0, C14:0, C16:0, C18:0 and C20:0 and variations in monounsaturated FA attributed to C16:1 n-7 and C18:1 n-9. For PUFA profiles, all six species had significantly different n-6/n-3 ratios, while the green seaweed Ulva species possessed the lowest n-6/n-3 ratio of 0.4, along with a 2-fold higher C18:3 n-3 to C18:2 n-6 content. Ulva sp. was the only species that contained docosahexaenoic acid. Extracts of both the Ulva sp. and Hormosira banksii showed selective cytotoxicity towards a human pancreatic cancer cell line, while the nonpolar extracts of all six algae species strongly inhibited production of the inflammatory-mediator nitric oxide. Abstract Increasingly macroalgae are being recognized as a growth opportunity for functional foods and nutritional security in the future. Dominating traits of interest are metabolites that function as anti-inflammatories and are antiproliferative. However seaweeds cultivated in the northern hemisphere dominate this field of research. Australia has a unique flora of macroalgae and it is poorly understood which species should be targeted for cultivation towards food and health markets. Herein, six Australian marine macroalgae were selected for screening of one antiinflammatory group; n-3 polyunsaturated fatty acids (PUFA). PUFA profiles were determined using gas chromatography -mass spectrometry and multivariate analysis. Thirty-one fatty acids (FA) were identified across the six macroalgal species with C16:0 the dominant FA in all samples, variations across taxa in the saturated FA C10:0, C14:0, C16:0, C18:0 and C20:0 and variations in monounsaturated FA attributed to C16:1 n-7 and C18:1 n-9. For PUFA profiles, all six species had significantly different n-6/n-3 ratios, while the green seaweed Ulva sp. possessed the lowest n-6/n-3 ratio of 0.4, along with a 2-fold higher C18:3 n-3 to C18:2 n-6 content. Ulva sp. was the only species that contained docosahexaenoic acid. Extracts of both the Ulva sp. and H. banksii showed selective cytotoxicity towards a human pancreatic cancer cell line, while the non-polar extracts of all six algal species strongly inhibited production of the inflammatory-mediator nitric ...

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Section: Discussionmentioning

confidence: 99%

Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity

et al. 2014

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“…Many species of macro algae had foremost constituentslike tetradecanoic acid, hexadecanoic acid, octadecanoic acid methyl esters etc (Balamurugan et al, 2013) which may reveal antagonism against marine bacteria (Al-Saif et al, 2013). Lately, the secondary metabolites and organic extracts obtained from U. fasciata has potential applications (Silva et al, 2013b) and the diverse derivatives of diterpenoids extracted from U. fasciata exhibited antagonism against Vibrio parahaemolyticus and V. harveyi (Chakraborty et al, 2010). Thus, in the present study, biological activity of U. fasciata against V. harveyi was due to the presence of various chemical constituents as described.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of marine macro alga, Ulva fasciata against bio-luminescent causing Vibrio harveyi during Penaeus monodon larviculture

Sivakumar¹,

Kannappan²,

Dineshkumar³

et al. 2014

Afr. J. Microbiol. Res.

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Vibrio harveyi is one of the major disease causing bacterium in shrimp larviculture and grow-out practices. V. harveyi produces many virulence cum pathogenic factors. Application of antibiotics against luminescence causes development of antibiotic resistance among V. harveyi. Therefore, it is obligatory to develop bio-inhibitory agents as substitute in lieu of antibiotics. Under this study, Ulva fasciata was collected and extracted for crude compounds, 300 µg extract showed 12.3 mm of bioinhibition against V. harveyi through "agar well diffusion assay". Further, U. fasciata extract at 300 µg/ml was treated against V. harveyi in LB broth and showed reductions on phospholipase and proteolysis. Production of bio-luminescence was reduced to 7.3, 7.7, 13.0, 17.0 counts per second (CPS) and growth also reduced to 24.91%. Further, U. fasciata extract at 200 g/ml was tested against V. harveyi during Penaeus monodon larviculture and showed 32.40% reduction in the cumulative percentage mortality on postlarvae due to V. harveyi. Chemical constituents of U. fasciata was characterized by FTIR and GCMS. GC-MS analysis, reported to contain organic compounds such as Bis(2-ethylhexyl) phthalate was highest (88.42%), followed by 1,2-benzenedicarboxylic acid-butyl (2.47%). Therefore, it was concluded that U. fasciata may be a better bio-inhibitory agent against V. harveyi in shrimp larviculture.

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“…Among all macroalgae, the green algae with less than 300 known compounds are the least producers of natural compounds when compared to the red (Rhodophyta) and brown algae (Phaeophyta) [9,10]. Anyhow, a wide range of compounds, predominantly terpenes, polyphenols and steroids, have been reported in various marine green algae [11,12]. The chemical composition of these macroalgae was found to vary depending on geographical distribution and seasons and the principal environmental factors affecting the composition being water temperature, salinity, light, nutrients and minerals availability [5].…”

Section: Secondary Metabolitesmentioning

confidence: 99%

The Marine Macroalgae of the Genus Ulva: Chemistry, Biological Activities and Potential Applications

Silva¹,

Vieira²,

Almeida³

et al. 2013

Oceanography

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Antibacterial labdane diterpenoids of Ulva fasciata Delile from southwestern coast of the Indian Peninsula

Cited by 90 publications

References 26 publications

Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity

Selecting Australian marine macroalgae based on the fatty acid composition and anti-inflammatory activity

Evaluation of marine macro alga, Ulva fasciata against bio-luminescent causing Vibrio harveyi during Penaeus monodon larviculture

The Marine Macroalgae of the Genus Ulva: Chemistry, Biological Activities and Potential Applications

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