Antibacterial Efficiency of Surface-Immobilized <i>Flavobacterium</i>-Infecting Bacteriophage

Leppänen, Miika; Maasilta, I. J.; Sundberg, Lotta‐Riina

doi:10.1021/acsabm.9b00242

Cited by 12 publications

(25 citation statements)

References 38 publications

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“…Efforts have been made to propose methods for phage coating in medical devices [ 33 , 34 , 35 ]. For indwelling urological devices, the phage-coating is usually obtained by physical adsorption [ 33 , 36 ] and hydrogel conjugation [ 34 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

et al. 2021

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Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail’s ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm.

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Section: Discussionmentioning

confidence: 99%

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

et al. 2021

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“…For example, Tawil et al found that phages were immobilized on gold with double the density when cross-linked via L-cysteine compared to glutaraldehyde, but that no significant difference was found in the bound phages' lytic behavior 72 . Similarly, Leppänen et al rigorously compared infectivity and phage surface density after surface treatments with different combinations of 11-MUA and (3-aminopropyl)triethoxysilane (APTES), concluding that a higher phage density did not always result in a higher infectivity 95 . Indeed, Naidoo et al…”

Section: A Note On Surface Densitymentioning

confidence: 99%

Strategies for Surface Immobilization of Whole Bacteriophages: A Review

O’Connell

Marcoux

Roupioz

2021

ACS Biomater. Sci. Eng.

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Bacteriophage immobilization is a key unit operation in emerging biotechnologies, enabling many new possibilities for biodetection of pathogenic microbes at low concentration, production of materials with novel antimicrobial properties, and fundamental research on bacteriophages themselves.Wild type bacteriophages exhibit extreme binding specificity for a single species -and often for a particular subspecies -of bacteria. Since their specificity originates in epitope recognition by capsid proteins, which can be altered by chemical or genetic modification, their binding specificity may also be redirected towards arbitrary substrates or a variety of analytes in 2 addition to bacteria. The immobilization of bacteriophages on planar and particulate substrates is thus an area of active and increasing scientific interest. This review assembles the knowledge gained so far in the immobilization of whole phage particles, summarizing the main chemistries and presenting the current state-of-the-art both for an audience well-versed in bioconjugation methods as well as for those who are new to the field.

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“…However, differences in the orientation of phages on the feed pellets and their detachment and infectivity after the corona discharge and spray treatment, respectively, might also affect the efficiency of delivery. Leppänen et al (2019) recently showed that the antimicrobial efficiency was higher for detached phages compared to the attached ones and that covalently bound phages on carboxylate-treated gold had the lowest infectivity even if this surface was characterized by the highest number of attached phages. The authors suggest that the lost infectivity of covalently bound phages might have been caused by chemical interactions or the improper orientation of the phages on the surface ( Leppänen et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“… Leppänen et al (2019) recently showed that the antimicrobial efficiency was higher for detached phages compared to the attached ones and that covalently bound phages on carboxylate-treated gold had the lowest infectivity even if this surface was characterized by the highest number of attached phages. The authors suggest that the lost infectivity of covalently bound phages might have been caused by chemical interactions or the improper orientation of the phages on the surface ( Leppänen et al, 2019 ). In our study, it does not seem that the covalently bound phages on feed pellets by the corona discharge method are characterized by a lower infectivity compared to phages applied using a spraying approach, as indicated by a very similar and stable phage number in the fish intestine when fed with the two phage-feed types.…”

Section: Discussionmentioning

confidence: 99%

Phage-Mediated Control of Flavobacterium psychrophilum in Aquaculture: In vivo Experiments to Compare Delivery Methods

et al. 2021

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Phage-based approaches have gained increasing interest as sustainable alternative strategies to antibiotic treatment or as prophylactic measures against disease outbreaks in aquaculture. The potential of three methods (oral, bath, and injection) for delivering a two-component phage mixture to rainbow trout fry for controlling Flavobacterium psychrophilum infections and reduce fish mortality was investigated using bacteriophages FpV4 and FPSV-D22. For the oral administration experiment, bacteriophages were applied on feed pellets by spraying (1.6 × 108 PFU g–1) or by irreversible immobilization (8.3 × 107 PFU g–1), using the corona discharge technology (Fixed Phage Ltd.). The fish showed normal growth for every group and no mortality was observed prior to infection as well as in control groups during the infection. Constant detection of phages in the intestine (∼103 PFU mg–1) and more sporadic occurrence in kidney, spleen, and brain was observed. When fish were exposed to F. psychrophilum, no significant effect on fish survival, nor a direct impact on the number of phages in the sampled organs, were detected. Similarly, no significant increase in fish survival was detected when phages were delivered by bath (1st and 2nd bath: ∼106 PFU ml–1; 3rd bath: ∼105 PFU ml–1). However, when phages FpV4 and FPSV-D22 (1.7 × 108 PFU fish–1) were administered by intraperitoneal injection 3 days after the bacterial challenge, the final percent survival observed in the group injected with bacteriophages FpV4 and FPSV-D22 (80.0%) was significantly higher than in the control group (56.7%). The work demonstrates the delivery of phages to fish organs by oral administration, but also suggests that higher phage dosages than the tested ones may be needed on feed pellets to offer fish an adequate protection against F. psychrophilum infections.

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Antibacterial Efficiency of Surface-Immobilized Flavobacterium-Infecting Bacteriophage

Abstract: This is a self-archived version of an original article. This version may differ from the original in pagination and typographic details.

Cited by 12 publications

References 38 publications

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

Strategies for Surface Immobilization of Whole Bacteriophages: A Review

Phage-Mediated Control of Flavobacterium psychrophilum in Aquaculture: In vivo Experiments to Compare Delivery Methods

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