Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis

Owais, M.; Sharad, K. Sharma; Shehbaz, A.; Saleemuddin, M.

doi:10.1016/j.phymed.2003.07.012

Cited by 158 publications

(111 citation statements)

References 15 publications

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“…[36,55] Therefore, our results are in accordance with these earlier reports, and the therapeutic activity of W. somnifera was also supported by its numerous bioactive components, including withaferin A, sitoindosides VII-X, 5-dehydroxy withanolide-R, withasomniferin-A, [56,57] which might reverse the damage caused in renal cells.…”

Section: Discussionsupporting

confidence: 92%

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Jeyanthi

Subramanian

2009

Renal Failure

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In the present study, we investigated the protective effect of Withania somnifera, an indigenous medicinal herb used in ayurvedic traditional systems for more than 3000 years in India, on gentamicin (GEN)-induced nephrotoxicity. The root extract of three different doses of W. somnifera (viz., 250, 500, and 750 mg/kg) was administered orally to rats for 14 days before GEN treatment and thereafter concurrently with GEN (100 mg/kg) for 8 days. Nephrotoxicity was evident in GEN-treated rats by significant increase in kidney weight, urea, creatinine, urinary protein, and glucose, and significant reduction in body weights and potassium, which was histopathologically confirmed by tubular necrosis. In contrast W. somnifera (500 mg/kg) significantly reversed these changes as evidenced microscopically when compared to other two doses of W. somnifera (250 and 750 mg/kg), and there were no significant changes in the levels of sodium in the experimental animals compared to control. Thus, our results suggested the nephroprotective effect of Withania somnifera, which could be by enhancing antioxidant activity with natural antioxidants and scavenging the free radicals.

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Section: Discussionsupporting

confidence: 92%

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Jeyanthi

Subramanian

2009

Renal Failure

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“…In the Ayurveda system of medicine, Withania is noted for its aphrodisiac, sedative and life-prolonging properties (8). Extracts from different parts of W. somnifera have been evaluated for various biological activities, such as cholinesterase inhibition (9,10), anti-inflammatory properties via cyclooxygenase-2 enzyme inhibition from leaf extracts (11), antibacterial properties (12) and sex hormone deficiency regulation in rats with diabetes (13). The isolation and identification of several withanolides with anti-glycation (14) and antipyretic activities (15) from Withania has been reported.…”

Section: Introductionmentioning

confidence: 99%

Antiproliferation potential of withaferin A on human osteosarcoma cells via the inhibition of G2/M checkpoint proteins

Wang

2015

Experimental and Therapeutic Medicine

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Abstract. Withaferin A (WA) is a well-known steroidal lactone of the medicinally important plant, Withania somnifera. This secondary metabolite has been noted for its anticancer effects against a number of human cancer cell lines. However, there are a limited number of studies investigating the growth inhibitory potential of WA against human osteosarcoma cells and the underlying molecular mechanisms. Thus, in the present study, the antiproliferative activities of WA, along with the underlying mechanisms of action, were investigated using flow cytometry for cell cycle distribution and western blot analysis for the assessment of various checkpoint proteins. In addition, the antiproliferative activity was evaluated using a sulforhodamine B assay, where MG-63 and U2OS human osteosarcoma cell lines were treated with different concentrations of WA. Furthermore, the mRNA expression levels of the checkpoint proteins in the WA-treated MG-63 and U2OS cells were examined. The results obtained corresponded with the western blot analysis results. Furthermore, WA was shown to significantly inhibit the proliferation of the two types of treated cell lines (MG-63 and U2OS). Flow cytometric analysis revealed that WA induced cell cycle arrest at the G2/M phase, which was associated with the inhibition of cyclin B1, cyclin A, Cdk2 and p-Cdc2 (Tyr15) expression and an increase in the levels of p-Chk1 (Ser345) and p-Chk2 (Thr68). In conclusion, the present study found that the antiproliferative potential of WA was associated with the induction of cell cycle arrest at the G2/M phase, which was a result of the attenuation of the expression levels of G2/M checkpoint proteins.

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“…Components such as ashwagandha, zinc, selenium, and magnesium were reported with improved platelet count, red blood cell, Hb, etc. [55][56][57][58]. Experimental results showed the herbomineral formulation improved the hematological animal profile, while Biofield Energy Healing Treatment on test formulation significantly improved the blood profile compared with the untreated test formulation.…”

Section: Hematological Analysismentioning

confidence: 95%

“…zinc, magnesium, and selenium as a basis to investigate ways to improve its immunomodulatory activity. All the components of test formulation are reported with various pharmacological activities like ashwagandha root extract [13][14][15][16], and the minerals [17].…”

Section: Introductionmentioning

confidence: 99%

Immunological Effects of Biofield Energy Healing (The Trivedi Effect<sup>®</sup>) Based Novel Herbomineral Formulation After Oral Administration in Male <i>Sprague Dawley</i> Rats

Trivedi¹

2017

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Herbomineral formulations have been used and accepted all around the world owing to its medicinal and therapeutic importance. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect ® ) Treatment on the new proprietary herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The Biofield Energy Treated and untreated test formulation was evaluated using experimental parameters such as humoral immune response (primary and secondary antibody titre), delayed type hypersensitivity reaction, animal weight parameters, feed and water intake, histopathology, hematological and serum biochemistry were studies. Humoral immune data showed the primary and secondary antibody titre values were significantly (p≤0.05) increased by 105.88% and 260%, respectively in the Biofield Energy Treated Test formulation (G4) group with respect to the disease control group (G2). The delayed type hypersensitivity results showed a significant increased paw edema by 32.43% and 53.33% at 24 and 48 hours, respectively in the Biofield Energy Treated Test formulation (G4) group, while untreated test formulation group (G5) animals showed 13.33% increase in the paw edema (at 48 hour) compared with the G2 group. Hematological analysis showed a significant increase in the red blood cell count, while the platelet count was significantly increased by 27.78% in Biofield Energy Treated Test formulation (G4) group as compared with the G2 group. Biochemical analysis exhibited an increased level of calcium and phosphorus by 1.58% and 12.65%, respectively while serum uric acid was significantly decreased by 36.66% in the G4 group compared with the G2 group. In addition, animal weight parameters like body weight, relative organ weight, histopathology, water and feed intake data suggest that the Biofield Energy Treated herbomineral formulation was found safe, non-toxic, throughout the exposure period. In conclusion, the The Trivedi Effect ® -Biofield Energy Healing based herbomineral formulation would be the powerful immunomodulatory product, which can be useful to modulate the immunity against many infectious diseases. Overall, experimental data suggests that the Biofield

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Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis

Cited by 158 publications

References 15 publications

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Antiproliferation potential of withaferin A on human osteosarcoma cells via the inhibition of G2/M checkpoint proteins

Immunological Effects of Biofield Energy Healing (The Trivedi Effect<sup>®</sup>) Based Novel Herbomineral Formulation After Oral Administration in Male <i>Sprague Dawley</i> Rats

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Antibacterial efficacy of Withania somnifera (ashwagandha) an indigenous medicinal plant against experimental murine salmonellosis

Cited by 158 publications

References 15 publications

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Antiproliferation potential of withaferin A on human osteosarcoma cells via the inhibition of G2/M checkpoint proteins

Immunological Effects of Biofield Energy Healing (The Trivedi Effect&lt;sup&gt;®&lt;/sup&gt;) Based Novel Herbomineral Formulation After Oral Administration in Male &lt;i&gt;Sprague Dawley&lt;/i&gt; Rats

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Immunological Effects of Biofield Energy Healing (The Trivedi Effect<sup>®</sup>) Based Novel Herbomineral Formulation After Oral Administration in Male <i>Sprague Dawley</i> Rats