Antibacterial Efficacy of Raw and Processed Honey

Abstract

In vitro antibacterial activity of methanol, ethanol, and ethyl acetate extracts of raw and processed honey was tested against Gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Enterococcus faecalis, and Micrococcus luteus) and Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi). Both types of honey showed antibacterial activity against tested organisms with the zone of inhibition (ZOI) ranging from 6.94 to 37.94 mm, while E. coli, S. typhi, and P. aeruginosa showed that sensibility towards all the extracts with ZOI ranges between 13.09 to 37.94 mm. The methanol extract showed more potent activity than other organic extracts. Gram-negative bacteria were found to be more susceptible as compared to Gram-positive bacteria except E. faecalis. The broth microdilution assay gave minimum inhibitory concentrations (MIC) value of 625 μg/mL, while the minimum bactericidal concentration (MBC) ranges between 625 μg/mL 2500 μg/mL. The study showed that honey has antibacterial activity (bacteriostatic and bactericidal effect), similar to antibiotics, against test organisms and provides alternative therapy against certain bacteria.

Antibacterial activity of three Cameroonian honey types against some pathogenic species

“…The different honeys possessed potential antibacterial activity against Gram negative bacterium (E. coli). This is in accordance with the previous report by Mohapatra et al (2011), indicating that raw and processed honeys have potential activity against this bacterium. Oku honey had moderate growth inhibitory effects on P. aeruginosa, K. pneumoniae and S. aureus.…”

Phytochemical screening and antimicrobial activity of apiary honey produced by honey bee (Apis mellifera) on clinical strains of Staphylococcus aureus, Escherichia coli and Candida albicans

“…The multi facet properties of honey anchored in the scientific world is regarded as a sweetener, functional food, antioxidant, antimicrobial, antiseptic, prebiotics, probiotics, immunomodulatory, antiinflammatory, anti-tumor and anti-cancer effect amongst others (Jenkins et al, 2011;Conway et al, 2010;Fauzi et al, 2011). Above and beyond its therapeutic effects or medicinal attributes (Mohapatra et al, 2011;Conti et al, 2007), it is also of potential use as bio-indicators for environmental contamination (Celechovska and Vorlova, 2001). The colour of honey can vary from nearly colourless to dark brown and its consistency can be fluid, viscous or partly to entirely crystallized.…”

Neonatal Fournier’s gangrene; sequelly of Traditional birth practice: Case report and Short Review

“…One of them is debridement 7&11 which was known to be very effective by Darke and its group in treatment of mixed Clostridia and non-Clostridia infected gangrene .This was the main course of treatment employed in our patient. Furthermore, Darke et 12 . One more emphasis is the use of adequate prophylaxis with penicillin 7 in patients at risk as stated by Darke.…”