Antibacterial efficacy of nisin, calcium hydroxide, and triple antibiotic paste in combination with chitosan as an intracanal medicament against Enterococcus faecalis – An in vitro study

Harshitha, VS; Ranjini, MA; Nadig, RoopaR

doi:10.4103/jcd.jcd_125_22

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…CH when combined with different vehicles has shown long-term release of calcium ions. [ 7 8 19 ] Hence, in our study, NCH was combined with NCH to study its effects on root dentin. Results showed that the combination (NCS + NCH) had better fracture resistance and depth of penetration in comparison to curcumin and its nano form.…”

Section: Discussionmentioning

confidence: 99%

“…As CS has mucoadhesive properties, whether it has a similar adhesive property to root canal dentin needs to be investigated because this property might help in the prolonged action of medicament within the root canal system. [ 8 ] This was the preliminary study determining the fracture resistance of this group; hence, there is no evidence in the literature. To validate these results, further in vitro studies need to be done.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown an additive effect when Ca(OH) 2 was combined with CS against Candida albicans and E. faecalis . [ 7 8 ]…”

Section: Introductionmentioning

confidence: 99%

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Comparative assessment of nanosized intracanal medicaments on penetration and fracture resistance of root dentin – An in vitro study

Prashanth,

Revankar,

Karale

et al. 2024

Journal of Conservative Dentistry and Endodontics

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Context: The antimicrobial potential of conventional medicaments is reduced due to their limited permeability. The use of calcium hydroxide (CH) is known to reduce the root strength. Nano intracanal medicaments have the following advantages over conventional such as higher surface area and chemical reactivity, and due to its nanosize, have better penetrability, supporting its possible use as an intracanal medicament. Nano versions of CH, chitosan (CS), CS + CH, curcumin (T), and its conventional forms are used in our study. Aim: The aim of this study was to evaluate and compare the depth of penetration of nanosized intracanal medicaments and their effect on fracture resistance of root dentin. Materials and Methods: Eighty extracted single-rooted teeth were used after decoronation. Canals were enlarged up to size 30 (0.04° taper) using Neoendo instruments (Orikam, India). Teeth were randomly assigned to two broad groups based on the type of intracanal medicament used; Group A: control (conventional) (n = 40) and Group B: nanosized intracanal medicaments (n = 40). Each group was subdivided into four experimental groups (n = 10), Group A1: CH, Group A2: CS, Group A3: curcumin (T), Group A4: CS + CH, Group B1: nano calcium hydroxide (NCH), Group B2: nano CS (NCS), Group B3: nano curcumin (NT), and Group B4: NCS + nano calcium hydroxide (NCS + NCH). All the specimens were stored in a humidor at 37°C for 4 weeks. Out of 10 specimens from each group, five specimens were used for evaluating the depth of penetration using a confocal laser scanning electron microscope, and the remaining five specimens were used for evaluating fracture resistance in a universal testing machine at the end of 4 weeks. Data were analyzed using one-way ANOVA and intergroup comparison using Tukey’s post hoc multiple comparison test. Results: The highest fracture resistance was seen with NCS and the maximum depth of penetration with nano calcium hydroxide (NCH). Conclusion: The fracture resistance and depth of penetration at a 4-week interval were higher in nano forms compared to their micro-sized counterparts with all groups. The coronal section presented the highest depth of penetration of intracanal medicaments followed by the middle and least in the apical section of root dentin with all groups.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparative assessment of nanosized intracanal medicaments on penetration and fracture resistance of root dentin – An in vitro study

Prashanth,

Revankar,

Karale

et al. 2024

Journal of Conservative Dentistry and Endodontics

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“…There are several intracanal medications that have been utilised, but some of the more popular ones include calcium hydroxide, a variety of medicines combinations such as formocresol, eugenol, and iodine potassium iodide etc. 4 Inflammatory responses in the tissues that are connected to injury & the type of harm are what cause endodontic discomfort. Endodontic discomfort might develop prior to, during, or following the endodontic procedure.…”

Section: Introductionmentioning

confidence: 99%

Inter-appointment endodontic pain comparison between calcium hydroxide and triple antibiotic paste

Bilal,

Naeem,

Ijaz

et al. 2023

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One of the biggest sources of dread for patients and a top concern for dentists is the anticipation and reality of root canal-related pain.1 Pain is defined as "an unpleasant sensory and emotional experience associated with actual or potential damage or described in terms of such damage" by the International Associates for the Study of Pain.2 The imbalance in the host-bacteria connection brought on by intra canal operations is the main cause of inter appointment pain. Objectives: The goal of current study was to associate the outcomes of these intracanal medications on pain experienced between endodontic appointments. This would make it easier for us to find better medications to treat discomfort between appointments. Methodology: For a period of six months, from January 6, 2022, to January 1, 2023, this randomized controlled study was carried out in the Dept. of Operative Dentistry at the MIHS in Islamabad, Pakistan. With a 5% level of significance, an 85% power of the trial, and a fraction of effectiveness of 72% with triple antibiotic paste and 38% with calcium hydroxide, a sample size of 80 people, 40 in each group, was estimated.

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“…Previous studies were extensively conducted on chitosan as an intracanal medicament and tested its effectiveness towards various root canal pathogens [25,26]. However, reports are lacking on the CH and chitosan combinations [19,27], and testing its efficacy against the specific root canal pathogens.…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of antimicrobial efficacy of different combinations of calcium hydroxide against Enterococcus faecalis

Teja,

Janani,

Srivastava

et al. 2023

BMC Oral Health

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Background The study aims to compare the synergistic antibacterial efficacy of different combinations of calcium hydroxide as an intracanal medicament against E. faecalis. Material and methods The current study included four hundred extracted human permanent mandibular premolar teeth. After complete chemo-mechanical preparation, the middle third of the root was sectioned using a rotary diamond disc and a total of 400 samples were obtained. The specimens were inoculated with E. faecalis for 21 days. After that, specimens were divided into five groups (n = 80) based on materials used for the disinfection of samples: Group I, calcium hydroxide alone; Group II, calcium hydroxide + 2% chlorhexidine gel; Group III, calcium hydroxide + 2% chitosan gel; Group IV, calcium hydroxide + 0.02% silver nanoparticle gel; Group V, calcium hydroxide + Bioactive glass S53P4. Dentin shavings from the apical third were obtained from the inner third of dentin were obtained using gates glidden no.1 to the apical depth, followed by no.2, 3, 4 and 5 analyzed for E. faecalis using the culture method. One-way analysis of variance (ANOVA) was used for data analysis, followed by post-hoc Tukey's test for multiple comparisons of means to check the difference in bacterial inhibition between the groups. Results ANOVA results revealed a significant reduction of bacterial counts in all the groups compared (p < 0.001). Intergroup comparison showed maximum bacterial reduction (p < 0.001) with calcium hydroxide + bioactive glass S53P4 compared with other groups. Conclusion Synergistic effect of calcium hydroxide showed better bacterial reduction compared to calcium hydroxide alone. Among the combinations evaluated, calcium hydroxide with bioactive glass, found to be most effective compared to other groups.

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Antibacterial efficacy of nisin, calcium hydroxide, and triple antibiotic paste in combination with chitosan as an intracanal medicament against Enterococcus faecalis – An in vitro study

Cited by 6 publications

References 14 publications

Comparative assessment of nanosized intracanal medicaments on penetration and fracture resistance of root dentin – An in vitro study

Comparative assessment of nanosized intracanal medicaments on penetration and fracture resistance of root dentin – An in vitro study

Inter-appointment endodontic pain comparison between calcium hydroxide and triple antibiotic paste

Comparative evaluation of antimicrobial efficacy of different combinations of calcium hydroxide against Enterococcus faecalis

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