Many species belonging to the Ficus genus (Moraceae) contain several active compounds such as flavonoids, tannins, sesquiterpenes, alkaloids, and saponins which possess biological activities such as antioxidant, anticancer, anti-inflammation, antiviral, antibacterial, and others. In this study, we evaluated the antimicrobial activity of the ethanolic extract derived from the leaves of Ficus lingua Warb. ex De Wild. & T.Durand against some Gram-positive and Gram-negative strains in order to evaluate the possible use of this plant in preventing infections caused by these bacteria both in veterinary and medicine. The leaves of Ficus lingua, cultivated under glasshouse conditions, were sampled at M.M. Gryshko National Botanic Garden (NBG), National Academy of Science of Ukraine. The testing of the antibacterial activity of the plant extracts was carried out in vitro by the Kirby-Bauer disc diffusion technique. In the current study, Gram-negative strains such as Escherichia coli (Migula) Castellani and Chalmers (ATCC ® 25922™), Escherichia coli (Migula) Castellani and Chalmers (ATCC ® 35218™), Pseudomonas aeruginosa (Schroeter) Migula (ATCC ® 27853™) and Gram-positive strains such as Staphylococcus aureus subsp. aureus Rosenbach (ATCC ® 29213™), Staphylococcus aureus subsp. aureus Rosenbach (ATCC ® 25923™), methicillin-resistant (MRSA), mecA positive Staphylococcus aureus (NCTC ® 12493), Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC ® 51299™) (resistant to vancomycin; sensitive to teicoplanin) and Enterococcus faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC ® 29212™) were used. Results of the current study revealed that both Gram-positive and Gram-negative strains were sensitive to the F. lingua extract. Gram-positive strains such as S. aureus subsp. aureus Rosenbach (ATCC ® 29213™), S. aureus subsp. aureus Rosenbach (ATCC ® 25923™), E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC ® 51299™) and E. faecalis (Andrewes and Horder) Schleifer and Kilpper-Balz (ATCC ® 29212™) were sensitive to the F. lingua extract. The highest diameters of inhibition zones after the application of the F. lingua extract were observed for S. aureus subsp. aureus strains. This study demonstrates the antibacterial potential of ethanolic extract derived from the leaves of F. lingua and for use in the treatment of bacterial infection. The bioactive compounds of F. lingua extract, as well as its main biological activities, make it a promising candidate for communicable disease management.