Antibacterial Effects of Hydro-alcoholic Extract of Pennyroyal, Cinnamon and Rhubarb on Klebsiella pneumoniae and Staphylococcus aureus: An In-vitro Study

Abstract

Background: Medicinal plants have been used for many different purposes in the past. These plants may produce antimicrobial metabolites that affect the natural growth of microorganisms. The aim of this research was to study the antibacterial properties of rhubarb, cinnamon and pennyroyal on Staphylococcus aureus and Klebsiella pneumoniae.
 Materials and Methods: In this study, the effect of hydro-alcoholic extract of pennyroyal, cinnamon and rhubarb on Staphylococcus aureus and Klebsiella pneumoniae was investigated after their collection and extraction process. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of this extract on bacteria were also measured.
 Results: The MIC of hydro alcoholic extract of pennyroyal was above 400 mg/ml for both Klebsiella pneumoniae and Staphylococcus aureus. Also the MBC has been reported 400 and 500 mg/dL, respectively. The MIC of cinnamon extract for Klebsiella pneumoniae and Staphylococcus aureus were reported 250 and higher and 500 and higher, respectively. Also, the MBC was reported 500 and 600 mg/ml, respectively. Klebsiella pneumoniae was resistant to rhubarb plant extract, and the MIC and the MBC for Staphylococcus aureus were reported 200 mg/ml and higher and 400 mg/ml and higher, respectively.
 Conclusion: The results of this study demonstrated that the hydro alcoholic extract of cinnamon, pennyroyal and rhubarb has anti bacterial effect against Klebsiella pneumoniae and Staphylococcus aureus.