Antibacterial effects of hybrid tooth colored restorative materials against Streptococcus mutans: An in vitro analysis

Abstract

Objective:The objective of this study was to evaluate the antibacterial effect of two hybrid restoratives, namely resin modified glass ionomer cement (GC Fuji II™ LC, GC Corporation, Tokyo, Japan) and giomer (Beautifil-II, Shofu Inc., Kyoto, Japan) against Streptococcus mutans [Microbial Type Culture Collection (MTCC), 890].Materials and Methods:The antibacterial effect was evaluated using an agar diffusion test. The prepared wells in petri dishes were completely filled with chlorhexidine (positive control group), resin modified glass ionomer cement and giomer respectively. Prepared bacterial suspension was poured over the petri dish and was spread evenly using the plate spreader. The culture plates were placed in the incubator for 24 h at 37°C. The antibacterial activity was evaluated after 24 h, 48 h, and 7 days for each group in triplicates.Results and Conclusion:The results of the antibacterial effect of the tested materials were collected, statistically analyzed using the ANOVA test to determine the difference between the mean diameters of the inhibition zone produced. The mean zone of bacterial inhibition was found to be more with the giomer specimens at all time periods. However, this inhibitory activity showed a gradual decrease over a period of 7 days and the maximum inhibition was evident after 24 h with both the test materials.

Theanalysis of cytotoxicity of an experimental preparation used for the reduction of dentin hypersensitivity

“…The results of the tests for 2 means are presented in Figs. [3][4][5][6]. P values are shown over the columns which reflect the compared means.…”

“…The results, including the mean value and standard deviation (SD) for the experimental and commercial formulations and each dilution (1 : 5, 1 : 10, 1 : 15), obtained after 24 h and 7 days, are presented in Tables (2, 3) and Figs. (3)(4)(5)(6). In the first step of the analysis, a comparison of the mean percentage of cytotoxicity in both experimental and commercial preparations obtained from the MTT assay after 24 h and 7 days was made.…”

“…Some authors use the strains of Streptococcus mutans to evaluate the influence of composite materials on various microbial metabolic effects. 5 Genotoxicity and carcinogenicity are very important parameters to be taken into account when assessing the impact of dental materials on living organisms. Genotoxicity is assessed based on the activity of nucleic acids transformed under the influence of test substances.…”

Effect of 15% Carbamide Peroxide on the Surface Roughness and Adhesion of Streptococcus mutans to Microhybrid Composite Resin and Giomer

“…37 In relation to the surface chemistry, it might be pointed out that release of fluoride from giomer 13,16,27,38,39 might result in a decrease in microbial adhesion. Some studies have shown that BA of giomer is less than that of other composite resins, which is attributed to the chemical structure of giomer.…”

“…16,27 In fact, giomer has an antibacterial effect, which is related to its fluoride release capacity. 13,16,27,38,39 It has been shown that specific monomers are released from composite resin that promote the growth of cariogenic bacteria. 7 Eick et al, 40 too, showed higher or similar BA of composite resins compared with ceramics and attributed it to the presence of specific monomers or fillers in composite resins that promote BA.…”