Antibacterial effect of the red sea soft coral Sarcophyton trocheliophorum

Abstract

The marine soft corals Sarcophyton trocheliophorum crude extracts possessed antimicrobial activity towards pathogenic bacterial strains, i.e. Bacillus cereus, Salmonella typhi, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Bioassay-guided fractionation indicated that the antimicrobial effect was due to the presence of terpenoid bioactive derivatives. Further biological assays of the n-hexane fractions were carried out using turbidity assay, inhibition zone assay and minimum inhibitory concentration for investigating the growth-inhibition effect towards the Gram-positive and Gram-negative bacteria. The fractions were screened and the structure of the isolated compound was justified by interpretation of the spectroscopic data, mainly mass spectrometry (GC-MS). The structure was assigned as (5S)-3-[(3E,5S)-5-hydroxy-3-hepten-6-yn-1-yl]-5-methyl-2(5H)-furanone and was effective at concentrations as low as 0.20 mg/mL. The above findings, in the course of our ongoing research on marine products, may implicate that the profound anti-microbial activity of the S. trocheliophorum soft corals, inhabiting the red sea reefs, is attributed to the presence of growth-inhibiting secondary metabolites mainly terpenoids.

Antibacterial Activity as Inhibitors Pathogen Bacterial on Pond Shrimp of Extract Marine Biota Collected From Maspari Island, South Sumatera, Indonesia

“…Sarcophyton sp. is similar, although its antibacterial activity is shown only with one isolate (Vibrio spp2) but indicates its antibacterial potential (Gomaa et al, 2016;Afifi et al, 2016;Rozirwan et al, 2015). The extract Aaptos sp.…”

“…This Sarcophyton has shown resistance to pathogenic bacteria such as S. aureus, Bacillus subtilis, E. coli, Vibrio cholera, Staphylococcus epidermidis, S Staphylococcus pneumonia, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter sp. (Al-Footy et al, 2015;Gomaa et al, 2016), While the genus of Aaptos has resistance with bacteria Pseudomonas fluorescens, Klebsiella oxytoca, Serratia marcescens, Salmonella enterica, Bacillus cereus and S. aureus (Beesoo et al, 2017).…”

Antibacterial activity from soft corals of the Red Sea, Saudi Arabia

“…(stalk) had a considerable effect in the inhibition of tested marine and human pathogenic bacteria. Sarcophyton is well known as a producer of sarcophine derivatives [28][29][30]. Sarcophine was isolated from S. glaucum collected from the Red Sea, with yields up to 3% of the animal dry weight [31].…”

“…The high activity allocated to the capitulum and stalk portions of Sarcophyton can be explained as these portions being the most valuable and having the greatest probability of being attacked or eaten following the optimal defence theory [32,33]. Various activities were displayed by the diterpene sarcophytolide isolated from the soft coral S. glaucum collected from Hurghada, Red Sea, Egypt [29]. Two hundred and five terpenes had been previously isolated from the genus Sarcophyton (16 species), demonstrating various biological features, such as antiviral, antifouling, antifeedant, and anti-inflammatory activities [34].…”

Pertumbuhan Karang Lunak Sarcophyton sp. yang Dibudidayakan di Teluk Awur, Jepara

“…Sarcophyton juga dinyatakan mengandung diterpenoid laut yang dapat dimanfaatkan sebagai agen anti-inflamasi (Gonzalez et al, 2015). Aktivitas anti-bakterial juga ditemukan pada S. trocheliophorum (Gomaa et al, 2015).…”