Antibacterial effect of essential oil vapours against different strains of Staphylococcus aureus, including MRSA

Abstract

The aim of this study was to identify antimicrobial properties of selected essential oils in the vapour phase against different strains of Staphylococcus aureus, including MRSA and resistant clinical isolates. The resistance pattern of used S. aureus strains was checked by disc diffusion method against three antibiotics. Essential oils tested were obtained by hydro-distillation and characterized by GC-MS and SPME-GC. Their antimicrobial properties in the vapour phase were determined by the microatmosphere method. Among the essential oils tested, the most active was Armoracia rusticana with MICs ranging from 8.3 to 17 µl/l, followed by Origanum syriacum (8.3-130 µl/l), Allium sativum (8.3-530 µl/l), Satureja hortensis (17-130 µl/l), Satureja montana (33-260 µl/l), Thymus vulgaris (33-260 µl/l), and Thymus serpyllum (33-530 µl/l). To the best of our knowledge, this is the first report of their antimicrobial properties in vapour phase against a collection of strains and clinical isolates of S. aureus, including MRSA. Based on the results, the essential oils tested can be considered as effective anti-staphylococcal natural products with specific application possibilities due to the activity in the vapour phase.

Medicinal plants of the family Lamiaceae as functional foods - a review

“…expectorant, pulmonary emphysema, urogenital diseases, immunostimulant (Ivanova et al 2005); anti-inflammatory, gastroprotective, and cytotoxic (Tadic et al 2012); antioxidant (Koleva et al 2003) leaves, flowers 45 Stachys byzantina C. Koch. antimicrobial, antioxidant, and antifungal (Conforti et al 2009 antibacterial (Nedorostova et al 2011;Joshi 2013) antibacterial, antioxidant, antimalarial, and antiproliferative (Hussain et al 2013);antioxidant and antihypertensive (Mihailovic-Stanojevic et al 2013) leaves 54 Thymus vulgaris L.…”

“…antioxidant (Cerda et al 2013);antifungal (Sellamuthu et al 2013);antimicrobial (Alinezhad et al 2013); antibacterial (Nedorostova et al 2011;Ballester-Costa et al 2013;Sfeir et al 2013 anti-diarrhea, febrifuge, and pectoral effects (Joudi et al 2011);antioxidant and antibacterial (Aliakbarlu & Shameli 2013) aerial parts Table 1 to be continued Within the aforementioned properties, a common need is the availability of natural extracts with preservative action, aimed to avoid oxidation and spoilage by microorganisms combined with pleasant taste or odour. Moreover, the scientific verification of the biological activity of plants with potential antimicrobial activities is needed, but unfortunately most of the medicinal and aromatic plants have not yet been exploited for their bioactivities.…”

A pharmacological and phytochemical overview onSatureja

“…Masumeh (2006) S. hortensis Abdollahi et al (2010Abdollahi et al ( , 2011Abdollahi et al ( , 2012, Adiguzel et al (2007), Aksu and Ozer (2013), Alizadeh-Salteh et al (2010b), Anghel et al (2013), Askun et al (2008), Azaz et al (2005), Azizi et al (2008), Bouari et al (2011), Boyraz and Ozcan (2006), Deans and Svoboda (1989), Dikbas et al (2008Dikbas et al ( , 2010Dikbas et al ( , 2012, Djenane et al (2011a), Fit et al (2009), Gulluce et al (2003), Gumus (2010), Gursoy et al (2009), Hosseini et al (2013), Karami-Osboo et al (2010), Kiran et al (2010), Kizil and Uyar (2006), Kotan et al (2012Kotan et al ( , 2013, Mahboubi and Kazempour (2009), Mihajilov-Krstev et al (2009a, Nabigol (2011), Nedorostova et al (2011), Oussalah et al (2006b), Ozcan et al (2008), RazzaghiAbyaneh et al (2008), Rezvanpanah et al (2011), Sagdic et al (2010Sagdic et al ( , 2013, Saharkhiz et al (2011), Sahin et al (2003), …”

Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya

“…Also, essential oils obtained from these species were utilized as flavor ingredients in a wide variety of foods, beverages and confectionery products, as well as in perfumery for the scenting of soaps and lotions. Several Thymus species are used as medicinal herbs, and they are known to possess antispasmodic, sedative, antiphlogistic, antiviral, antioxidant, antibacterial and antifungal activities [3][4][5][6][7][8][9][10][11].…”