Antibacterial effect of electrospun polycaprolactone/polyethylene oxide/vancomycin nanofiber mat for prevention of periprosthetic infection and biofilm formation

Jang, Chul Ho; Cho, Yong Beom; Jang, Yoon Seok; Kim, Min Seong; Kim, GeunHyung

doi:10.1016/j.ijporl.2015.05.037

Cited by 34 publications

(16 citation statements)

References 22 publications

(23 reference statements)

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“…A rabbit otitis model examined the efficacy of a nanoporous middle ear implant coating releasing ciprofloxacin against P. aeruginosa (370). In another study, a guinea pig animal model combined with otoendoscopy, histology, and bone CT was used to examine the inhibitory effect of a vancomycin-eluting nanofiber mat against MRSA biofilms formed on ossicular prostheses and middle ear infections (371).…”

Section: Vertebrate Animal Modelsmentioning

confidence: 99%

Options and Limitations in Clinical Investigation of Bacterial Biofilms

et al. 2018

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Bacteria can form single- and multispecies biofilms exhibiting diverse features based upon the microbial composition of their community and microenvironment. The study of bacterial biofilm development has received great interest in the past 20 years and is motivated by the elegant complexity characteristic of these multicellular communities and their role in infectious diseases. Biofilms can thrive on virtually any surface and can be beneficial or detrimental based upon the community's interplay and the surface. Advances in the understanding of structural and functional variations and the roles that biofilms play in disease and host-pathogen interactions have been addressed through comprehensive literature searches. In this review article, a synopsis of the methodological landscape of biofilm analysis is provided, including an evaluation of the current trends in methodological research. We deem this worthwhile because a keyword-oriented bibliographical search reveals that less than 5% of the biofilm literature is devoted to methodology. In this report, we (i) summarize current methodologies for biofilm characterization, monitoring, and quantification; (ii) discuss advances in the discovery of effective imaging and sensing tools and modalities; (iii) provide an overview of tailored animal models that assess features of biofilm infections; and (iv) make recommendations defining the most appropriate methodological tools for clinical settings.

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Section: Vertebrate Animal Modelsmentioning

confidence: 99%

Options and Limitations in Clinical Investigation of Bacterial Biofilms

et al. 2018

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“…The antibacterial efficacy of the PEP-1-loaded scaffolds was tested against EC and SA bacteria. In the current study, an agar diffusion method was adapted from the references with a little modification [39,41,42,43]. Firstly, SA and EC bacteria were cultured two passages in LB-broth after cell thawing from frozen storage.…”

Section: Methodsmentioning

confidence: 99%

Preparation and in Vitro Evaluation of New Composite Mesh Functionalized with Cationic Antimicrobial Peptide

et al. 2019

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Infection caused by bacteria in hernia repair site is a severe complication, and patients have to undergo a second surgery to remove the infected prosthesis. In this study, we developed a composite biological safe mesh with antibacterial activity. The composite mesh is composed of large pore polypropylene (PP) mesh, poly-caprolactone (PCL) and antimicrobial peptide (PEP-1), which we synthesized in our lab. Fourier transformed infrared (FTIR) spectroscopy was utilized to analyze the functional groups. The surface morphology, in vitro release characters, mechanical properties, antibacterial activities, and in vitro cytotoxicity of modified mesh were evaluated. Results showed that PEP-1 was loaded in fibers successfully and could diffuse from nanofibers to inhibit bacteria (E. coli) growth. However, the modified mesh did not show inhibition to S. aureus. The mechanical properties of fabricated mesh showed no difference with two commercial surgical meshes. What is more, modified mesh was proved to be nontoxic to human dermal fibroblasts, indicating that this method to fabricate meshes with antibacterial activity is feasible and provides a new strategy for the development of surgical meshes.

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“…A number of studies have verified that the reagents and techniques used to create the vancomycin–impregnated membrane will not change vancomycin’s configuration or physical properties. 23–26…”

Section: Methodsmentioning

confidence: 99%

“…A number of studies have verified that the reagents and techniques used to create the vancomycin-impregnated membrane will not change vancomycin's configuration or physical properties. [23][24][25][26] Electron microscopy and electrospinning parameters A PCL solution not containing vancomycin was used to prepare test membrane samples. This solution was added to the injector of an electrospinning machine, and electrospinning parameters (size of injection needle, receiving distance, and spinning voltage) were varied.…”

Section: Preparation Of a Resorbable Pcl Electrospun Fiber Membrane Cmentioning

confidence: 99%

Vancomycin–impregnated electrospun polycaprolactone (PCL) membrane for the treatment of infected bone defects: An animal study

Wei

Jian

et al. 2018

J Biomater Appl

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There is no consensus for the management of critical infected bone defects. The purpose of this study was to produce a vancomycin-impregnated electrospun polycaprolactone (PCL) membrane for the treatment of infected critical bone defects, and test it in a rabbit model. Electrospinning produced a resorbable PCL fiber membrane containing vancomycin approximately 1 mm in thickness, with a pore diameter of <10 μm. Femur defects were made in the limbs of 18 rabbits and infected with Staphylococcus aureus. The rabbits were divided into three groups according to treatment: (1) Experimental group: rabbit freeze-dried allogeneic bone graft and the vancomycin-PCL membrane. (2) Control group 1: bone graft. (3) Control group 2: vancomycin-PCL membrane only. Culture showed no difference in osteoclast activity between the three groups. Transwell testing showed that almost no fibroblasts passed through the membrane during the first 24 h, but some fibroblasts were able to pass it after 72 h. At 12 weeks after surgery, there was significantly less inflammatory cell infiltration in the experimental compared to the control groups. New bone formation and fracture bone callus were greater in the experimental group than control groups. We thus conclude the resorbable electrospun vancomycin-impregnated PCL membrane was effective at controlling bone infection, and in the regeneration of bone in a critical bone defect animal model.

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Antibacterial effect of electrospun polycaprolactone/polyethylene oxide/vancomycin nanofiber mat for prevention of periprosthetic infection and biofilm formation

Cited by 34 publications

References 22 publications

Options and Limitations in Clinical Investigation of Bacterial Biofilms

Options and Limitations in Clinical Investigation of Bacterial Biofilms

Preparation and in Vitro Evaluation of New Composite Mesh Functionalized with Cationic Antimicrobial Peptide

Vancomycin–impregnated electrospun polycaprolactone (PCL) membrane for the treatment of infected bone defects: An animal study

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