Antibacterial Effect of Crude Extracts of Kaempferia parviflora (Krachaidam) against Cronobacter spp. and Enterohemorrhagic Escherichia coli (EHEC) in Various Dairy Foods: A Preliminary Study

Jeong, Dana; Kim, Dong‐Hyeon; Chon, Jung‐Whan; Kim, Hyun‐Sook; Lee, Soo-Kyung; Kim, Hong-Seok; Yim, Jin-Hyuk; Song, Kwang‐Young; Kang, Il-Byung; Kim, Youngji; Park, Jin-Hyeong; Jang, Ho-Seok; Kang, Soo-Hyun; Kim, Soo-Ki; Seo, Kun‐Ho

doi:10.22424/jmsb.2016.34.2.63

Cited by 9 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, the application of antibacterial substances derived from natural sources has received considerable attention. Previous research indicated that an ethanolic KP extract could inhibit growth of the bacteria Staphylococcus aureus [ 4 ] and Escherichia coli [ 26 ], which are responsible for skin infections. In this study, electrospun shellac fibers containing KP extract were tested for antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

Fabrication, Optimization, and Characterization of Antibacterial Electrospun Shellac Fibers Loaded with Kaempferia parviflora Extract

et al. 2022

View full text Add to dashboard Cite

This study aimed to develop a Kaempferia parviflora (KP) extract based on electrospun shellac fibers capable of transporting methoxyflavones. This study used a Box–Behnken design to determine the optimal production parameters that influence the fiber diameter and bead-to-fiber ratio responses. The optimization step produced fibers with a small diameter (574 nm) and a lower bead-to-fiber ratio (0.48 beads per fiber) by combining 37.25% w/w shellac and 1.50% w/w KP extract with a solution feed rate of 0.8 mL/h and an electrical voltage of 18 kV. The KP extract was found to be dispersed throughout the electrospun shellac fibers during the characterization study. The results were highly correlated with the theoretical values, indicating that the regression models used to predict the response variables were adequate. A study of in vitro dissolution confirmed that KP extract-loaded electrospun shellac fibers could produce a sustained-release profile within 10 h. Additionally, KP-infused shellac fibers demonstrated antibacterial activity against Staphylococcus aureus. This KP loading method combined with shellac properties provided a new delivery system and could be used to explore novel biomedical materials.

show abstract

Section: Introductionmentioning

confidence: 99%

Fabrication, Optimization, and Characterization of Antibacterial Electrospun Shellac Fibers Loaded with Kaempferia parviflora Extract

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Methoxyflavone was identified as an active component in the KP rhizome with a variety of biological properties, while KP activities include the support of sexual performance and antioxidant, antitumor growth and metastasis, bactericidal and anti-inflammatory effects [20][21][22][23][24]. The ethanol extract of KP inhibits Cronobacter spp., enterohemorrhagic Escherichia coli and H. pylori growth, which are offensive gastro-enteric pathogens [25]. In a previous study, the crude ethyl acetate extract of KP (CEAE-KP) was shown to reduce H. pylori pathogenic function by inhibiting their internalization process in human laryngeal carcinoma cells [26].…”

Section: Introductionmentioning

confidence: 99%

Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

Nemidkanam

Kato

Chaichanawongsaroj

2020

BMC Complement Med Ther

View full text Add to dashboard Cite

Background: Kaempferia parviflora (KP) has been used in traditional Thai medicine to cure gastrointestinal disorders since ancient times. Helicobacter pylori is an initiating factor in gastric pathogenesis via activation of massive inflammation, the cumulative effect of which leads to gastric disease progression, including gastric carcinogenesis. Accordingly, the effect of a crude ethyl acetate extract of KP (CEAE-KP) on proinflammatory cytokine production and cell chemotaxis was the focus of this study. Methods: The cytotoxicity of CEAE-KP (8-128 μg/ml) on AGS (gastric adenocarcinoma) cells was determined at 6, 12 and 24 h using an MTT assay. The effect of CEAE-KP on H. pylori-induced interleukin (IL)-8 production by AGS cells was evaluated by ELISA and RT-PCR. The effect of CEAE-KP on monocyte and neutrophil chemotaxis to H. pylori soluble protein (sHP) and IL-8, respectively, was determined using a Boyden chamber assay with THP-1 or HL-60 cells. Results: CEAE-KP reduced AGS cell viability in a concentration-and time-dependent manner, but at 8-16 μg/ml, it was not cytotoxic after 6-24 h of exposure. Coculture of AGS cells with CEAE-KP at a noncytotoxic concentration of 16 μg/ml and H. pylori reduced IL-8 secretion by~60% at 12 h, which was consistent with the decreased level of mRNA expression, and inhibited neutrophil chemotaxis to IL-8. sHP (100 ng/ml) induced marked monocyte chemoattraction, and this was decreased by~60% by CEAE-KP. Conclusion: CEAE-KP might serve as a potent alternative medicine to ameliorate the inflammation mediated by H. pylori infection.

show abstract

“…[3][4][5][6][7] Kaempferia parviflora is one such medicinal plant of zingiberaceae whose rhizomes have been used as a remedy for many ailments by the native people of Manipur, North-East, India since time immemorial without proper scientific formulations. 8 The plant is locally known as Singmu in Manipuri and Thai ginseng or Black Ginger in Thailand. 9 Medicines derived from the rhizomes of Kaempferia parviflora have been reported to use for inflammation, hypertension, erectile dysfunction and abdominal ailments in Thai and Laos.…”

Section: Introductionmentioning

confidence: 99%

“…15 Also, a study reported that the plant possesses anti-plasmodial, antifungal and antibacterial activities. 8 In general, plant extract is considered to be the rich resource for isolating pharmacologically important natural products. 16 The phytoconstituent present in the plant extract vary significantly depending on the extraction method as well as polarity of the solvent used for extraction.…”

Section: Introductionmentioning

confidence: 99%

GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract

Atom¹,

Shaikh²,

Maharabam³

et al. 2022

Int J. Pharm. Investigation

View full text Add to dashboard Cite

Bakground: Kaempferia parviflora (zingiberaceae family) rhizomes are used for various pharmacological applications. The present study is aimed for putative identification of phyto-constituents in the chloroform extract of the rhizome of Kaempferia parviflora and to correlate with bioactivities like antioxidant and anti-microbial/fungal activities. Methods: Rhizome extract was prepared using Soxhlet extractor and undergone the phytoconstituents analysis of it by gas chromatography-mass spectrometry (GC-MS). Results: Identified twenty-five compounds, out of which 4H-1-Benzopyran-4-0ne,5,7-dimethoxy-2-phenyl and Hydroxyurea showed the maximum and minimum relative abundance of 76.4% and 0.002% respectively. By agar well diffusion method, the extract (25 μg/ml) exhibited highest antibacterial potential against Micrococcus luteus (MTCC 106) (gram-positive bacteria) and anti-fungal potential against plant pathogen Rhizoctonia solani (MTCC 4633). The extract also showed the potent scavenging activities of 2,2-diphenyl-1-picryl-hydrazine (DPPH) and 2,2′-Azino-bis (3-Ethylbenzothiazoline-6-Sulfonic Acid) (ABTS) radicals with IC 50 values of 3.0 ± 0.1 mg/ml and 19.4 ± 1.2 µg/ml respectively. Subsequently, lipid peroxidation assay was also used for estimation of antioxidant activity of this extract. The extract (20 µg/ml) inhibited lipid peroxidation by 80.0 ± 4.5%. Conclusion: The outcomes of present study provide scope for isolation of bioactive compounds from Kaempferia parviflora for medicinal application.

show abstract

Antibacterial Effect of Crude Extracts of Kaempferia parviflora (Krachaidam) against Cronobacter spp. and Enterohemorrhagic Escherichia coli (EHEC) in Various Dairy Foods: A Preliminary Study

Cited by 9 publications

References 17 publications

Fabrication, Optimization, and Characterization of Antibacterial Electrospun Shellac Fibers Loaded with Kaempferia parviflora Extract

Fabrication, Optimization, and Characterization of Antibacterial Electrospun Shellac Fibers Loaded with Kaempferia parviflora Extract

Ethyl acetate extract of Kaempferia parviflora inhibits Helicobacter pylori-associated mammalian cell inflammation by regulating proinflammatory cytokine expression and leukocyte chemotaxis

GC-MS Profiling, in vitro Antioxidant and Antimicrobial Activities of Kaempferia parviflora Wall. ex. Baker Rhizome Extract

Contact Info

Product

Resources

About