Antibacterial effect of bactericide immobilized in resin matrix

Namba, Naoko; Yoshida, Yasuhiro; Nagaoka, Noriyuki; Takashima, Seisuke; Matsuura-Yoshimoto, Kaori; Maéda, Hiroshi; Meerbeek, Bart Van; Suzuki, Kazuomi; Takashiba, Shogo

doi:10.1016/j.dental.2008.08.012

Cited by 146 publications

(166 citation statements)

References 20 publications

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“…The antimicrobial mechanism is that when the negatively charged bacterial cell comes into contact with the positively charged sites of QAS resin, the electrical balance of the cell membrane could be disturbed, and the bacterium could explode under its own osmotic pressure (Beyth et al, 2006;Namba et al, 2009). Uncured MDPB primer was antibacterial (Imazato et al, 1997), and its antibacterial effect was maintained after being photo-cured (Imazato et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

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Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

et al. 2012

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Antibacterial bonding agents could combat recurrent caries at the tooth-composite margins. The objectives of this study were to develop novel antibacterial dentin primers containing quaternary ammonium dimethacrylate (QADM) and nanoparticles of silver (NAg), and to investigate the effects on dentin bond strength and dental plaque microcosm biofilms for the first time. Scotchbond Multi-Purpose ("SBMP") bonding agent was used. QADM and NAg were incorporated into SBMP primer, yielding 4 primers: SBMP primer (control), control + 10% QADM (mass), control + 0.05% NAg, and control + 10% QADM + 0.05% NAg. Human saliva was collected to grow microcosm biofilms. The NAg particle size (mean ± SD; n = 100) was 2.7 ± 0.6 nm. Dentin shear bond strengths (n = 10) with human third molars were approximately 30 MPa for all groups (p > 0.1). QADMNAg-containing primer increased the bacteria inhibition zone by 9-fold, compared with control primer (p < 0.05). QADM-NAg-containing primer reduced lactic acid production and colony-forming units of total micro-organisms, total streptococci, and mutans streptococci by an order of magnitude. In conclusion, novel QADM-NAg-containing primers were strongly antibacterial without compromising dentin bond strength, and hence are promising to inhibit biofilms and secondary caries. The processing method of incorporating QADM and NAg together into the same primer produced the strongest antibacterial effect, which could have a wide applicability to other bonding systems.KEY WOrDs: antibacterial dentin primer, quaternary ammonium salt, dental biomaterials, bond strength, dental plaque microcosm biofilm, caries inhibition.

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Section: Discussionmentioning

confidence: 99%

“…To address this problem, investigators developed novel antibacterial composites (Imazato, 2003;Beyth et al, 2006;Namba et al, 2009;Fan et al, 2011). Quaternary ammonium salt (QAS) monomers, such as 12-methacryloyloxydodecylpyridinium bromide (MDPB), were effective in providing antibacterial properties (Imazato, 2003).…”

Section: Introduction Cmentioning

confidence: 99%

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

et al. 2012

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“…Namba et al 11) showed that a simple mixture of CPC with a light-curable resin led to growth inhibition of Streptococcus mutans attached to the resin surface, although no inhibitory effect was observed against bacteria that were not attached to the surface. Ehara et al 16) developed a resin with recoverable antibacterial activity based on the desorption/adsorption of CPC by the ion-exchange mechanism.…”

Section: Introductionmentioning

confidence: 99%

“…Direct restorative materials (such as composite resins and glass ionomer cements) with antibacterial properties have been developed [8][9][10][11][12] to avoid secondary caries resulting from plaque formation on restorative materials, which has been reported as the main cause of restoration failure 13) . Thus, the incorporation of antibacterial properties into nanoparticulate repair agents should be an effective method to restore the damaged enamel and prevent the formation of secondary caries; however, there have been few reports on the subject to date.…”

Section: Introductionmentioning

confidence: 99%

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies

et al. 2016

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Application of hydroxyapatite (HAp) nanoparticles to repair damaged enamel has attracted recent attention. In this study, HAp nanoparticles with various morphologies (spherical, short-rod, long-rod and fiber morphologies) were synthesized via chemical precipitation methods without the addition of template molecules, and the adsorption/desorption behaviors of a cationic antibacterial agent, cetylpyridinium chloride (CPC), on the HAp nanoparticles were evaluated. The adsorption of CPC on each HAp nanoparticle showed Langmuir-type adsorption, and the short-rod/long-rod HAp nanoparticles showed thermodynamically more stable adsorption of CPC than that with the spherical/fiber HAp nanoparticles. The desorption rate of CPC from the short-rod/long-rod HAp nanoparticles was slower than that of the spherical/fiber HAp nanoparticles. The HAp nanoparticles with different CPC release profiles presented here have potential applications as nanoparticulate enamel repair agents with antibacterial properties.

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“…The bacteria could then be damaged or killed by cytoplasmic leakage. 19,20 QAS-containing bonding agents act upon the bacteria that are closest to the resin surface layer. This is related to the contact killing mechanism, whose QAS is copolymerized with and immobilized in the resin.…”

Section: Introductionmentioning

confidence: 99%

Influence of addition of [2-(methacryloyloxy)ethyl]trimethylammonium chloride to an experimental adhesive

et al. 2017

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Influence of addition of [2-(methacryloyloxy)ethyl] trimethylammonium chloride to an experimental adhesiveAbstract: The aim of this study was to develop an experimental adhesive with addition of [2-(methacryloyloxy)ethyl]trimethylammonium chloride (METAC) and to evaluate its mechanical and biological properties and its in vitro antibacterial activity. An experimental adhesive resin was formulated with Bis-GMA, TEGDMA, and HEMA. The antibacterial monomer was added at concentrations of 1%, 2.5%, and 5% (METAC groups). A group without METAC addition was used as control. The experimental adhesives were evaluated as to their antibacterial potential against Streptococcus mutans, degree of conversion, and softening in ethanol for 2 hours. The data were analyzed by one-way ANOVA, Tukey's post-hoc test, and the paired Student's t-test (significance level of 0.05). METAC showed antibacterial activity against S. mutans at all concentrations (p < 0.05). There was no statistical difference across METAC groups (p > 0.05). The 1%, 2.5%, and 5% groups yielded the highest mean values for degree of conversion (p < 0.05). The 1% group did not differ from the control group (p > 0.05). There was no statistical difference in baseline microhardness values (p > 0.05) and microhardness values after immersion in ethanol were lower than at baseline for all groups (p < 0.05). There was no statistical difference in the reduction of Knoop hardness number (KHN) after immersion in ethanol for any of the groups (p > 0.05). The results of the present study indicate that METAC is a promising antibacterial agent when added to an adhesive system.

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Antibacterial effect of bactericide immobilized in resin matrix

Cited by 146 publications

References 20 publications

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

Anti-biofilm Dentin Primer with Quaternary Ammonium and Silver Nanoparticles

Adsorption and desorption behaviors of cetylpyridinium chloride on hydroxyapatite nanoparticles with different morphologies

Influence of addition of [2-(methacryloyloxy)ethyl]trimethylammonium chloride to an experimental adhesive

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