Antibacterial cationic poly(vinyl chloride) as an approach for in situ pathogen-inactivation by surface contact with biomedical materials

García

2020

J. Chil. Chem. Soc.

Self Cite

Mid-infrared spectroscopy in conjunction with Functionally-Enhanced Derivative Spectroscopy (IR+FEDS) is a powerful analytical tool for the improvement of analysis of microorganism IR spectra. The objective of this research is to characterize the outer surface of two Helicobacter pylori strains by IR+FEDS. This work is a key stage for the study of cell-cell and cell-surface interactions between microorganisms, as well as, for polymicrobial biofilm characterizations where H. pylori species are involved. Artificial bacterial biofilms were deposited on ultrafiltration cellulose membranes covalently modified by insertion of one spectral marker and used as sensing surface for analysis of bacterial biolayers. Biolayers were analyzed using an infrared spectrophotometer with ATR. Data were analyzed by classic procedures and by deconvolution based on FEDS transform. It is concluded that, for correct application of technique is required a minimum amount of noise in the working spectra which can be achieved by simple smoothing algorithms; in addition, reproducibility must be warranted by the implementation of standardized protocols and the use of an appropriate number of samples. It is concluded that in addition to typical signals associated with the IR spectrum of microorganisms, by FEDS, a better and more detailed description of the outer membrane of H. pylori biofilms can be performed. In particular, it is concluded that the detecting and monitoring of cysteine-rich proteins can be satisfactorily performed by IR+FEDS.

Section: Theoretical Backgroundmentioning

confidence: 99%

Vibrational Spectrum Characterization of Outer Surface of Helicobacter Pylori Biofilms by Functionally-Enhanced Derivative Spectroscopy (Feds)

García

2020

J. Chil. Chem. Soc.

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Advances in Polymer Technology

“…Their innovative approach involved grafting PVC onto poly hexamethylenediamine guanidine hydrochloride (PHMG) to create this remarkable compound [39]. Palencia et al [45] synthesized antibacterial cationic PVC by quaternization of amino groups using dimethylamine and 1-bromoethane, respectively. Feit et al [46] incorporated Snitroso-N-acetylpenicillamine (SNAP) into PVC tubing using a solvent-swelling-impregnation method to create an antimicrobial medical device interface.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Antibacterial Activity of Grafted Poly(Vinyl Chloride) Polymer against Gram-Positive and Gram-Negative Bacteria

Kotian,

Gupta,

Puttaiahgowda

et al. 2024

Infectious diseases caused by microorganisms have gained worldwide attention in recent years. According to data compiled by the World Health Organization, the number of deaths resulting from infectious diseases is on the rise. In light of these dangers, the study of antibacterial materials has become increasingly vital. In this research, an antibacterial polymer was developed using poly(vinyl chloride) (PVC) and 4,4-diamminodiphenylmethane (DDM). The produced polymer’s chemical structure and thermal properties were investigated using Fourier-transform infrared spectroscopy, nuclear magnetic resonance, and thermo-gravimetric analysis. The antibacterial activity of the resulting PVC-g-DDM polymer was effective in killing both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. The antimicrobial efficacy was tested using a spread plate method, demonstrating its potential utility in a variety of applications like biomedical, coatings, water purification systems, and others. Antimicrobial resistance is increasing, especially among bacteria that have acquired resistance to multiple therapeutics. To fully optimize and explore the polymer’s potential and its usage, more research is needed.

“…The main procedures to confer auto-disinfectant properties to PVC are surface modification and mixing inorganic materials with intrinsic bactericidal and fungicidal properties ( Behboudi et al, 2018 ). The blending with cationic polymers and functionalization with cationic groups (cationization) are other viable technological approaches to add bactericidal properties to PVC ( Palencia et al, 2019 ). The incorporation of antimicrobial agents in the polymer matrix may bring some advantages over other methods, such as the possibility of using conventional polymer processing equipment (extruders, injectors, among others) and longer time extension of the antimicrobial activity over time.…”

Section: Introductionmentioning

confidence: 99%

PVC containing silver nanoparticles with antimicrobial properties effective against SARS-CoV-2

et al. 2023

Poly (vinyl chloride) (PVC) is commonly used to manufacture biomedical devices and hospital components, but it does not present antimicrobial activity enough to prevent biofouling. With the emergence of new microorganisms and viruses, such as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) that was responsible for the global pandemic caused by Coronavirus Disease 2019 (COVID-19), it is evident the importance of the development of self-disinfectant PVC for hospital environments and medical clinics where infected people remain for a long time. In this contribution, PVC nanocomposites with silver nanoparticles (AgNPs) were prepared in the molten state. AgNPs are well-known as antimicrobial agents suitable for designing antimicrobial polymer nanocomposites. Adding 0.1 to 0.5 wt% AgNPs significantly reduced Young’s modulus and ultimate tensile strength of PVC due to the emergence of microstructural defects in the PVC/AgNP nanocomposites, but the impact strength did not change significantly. Furthermore, nanocomposites have a higher yellowness index (YI) and lower optical bandgap values than PVC. The PVC/AgNP nanocomposites present virucidal activity against SARS-CoV-2 (B.1.1.28 strain) within 48 h when the AgNP content is at least 0.3 wt%, suitable for manufacturing furniture and hospital equipment with self-disinfectant capacity to avoid secondary routes of COVID-19 contagion.