Antibacterial capacity of cavity disinfectants against <i>Streptococcus mutans</i> and their effects on shear bond strength of a self-etch adhesive

Abstract

We evaluated the antibacterial properties of three disinfectants [2% chlorhexidine (CHX), 6% sodium hypochlorite (NaOCl), and 0.01% urushiol)] against Streptococcus mutans and their effects on bond strength of Scotchbond TM Universal. The reduction in bacterial growth was evaluated by the colony counting method. Total 105 specimens were assigned to seven groups, according to surface pretreatment: control group (C) without pretreatment; chlorhexidine gluconate with rinse (CR) or without rinse (CD); NaOCl with rinse (NR) or without rinse (ND); and urushiol with rinse (UR) or without rinse (UD). The shear bond test was performed at a cross-head speed of 0.5 mm/min. None of the disinfected specimens had viable microbes after a 30 min incubation. The control group exhibited the strongest bond; however, no significant difference was detected with the disinfectant-treated groups, except weak bonding with ND group. These findings suggest that all disinfectants tested had strong antibacterial capacity and may better be rinsed away.

Effect of cavity disinfectants on antibacterial activity and microtensile bond strength in class I cavity

“…In this study, urushiol treatment also showed a similar microtensile bond strength to that of the control group, which is consistent with a previous study 22) . That study reported urushiol would remove the overlying contaminants (smear layer and smear plugs).…”

“…Thus, the first hypothesis, no difference in the antibacterial activity against S. mutans among the disinfectants, was accepted. Cha and Shin 22) reported that CHX, NaOCl, and urushiol exhibited potent antibacterial activity on S. mutans. They found that no S. mutans survived after 30 min of incubation with these disinfectants.…”

“…However, the antibacterial test was conducted in a Streptococcus mutans (S. mutans) inoculated brain-heart infusion (BHI) medium containing each disinfectant. Therefore, S. mutans in the planktonic state were the only target microorganisms 22) . Furthermore, polymerization shrinkage stress from the restricted cavity could not be considered when estimating bond strength.…”

“…Urushiol is also known to disrupt the bacterial cell membrane 21) . There was a study on the antibacterial characteristics of three cavity disinfectants (CHX, NaOCl, urushiol) and their effects on the shear bond strength of a selfetch adhesive 22) . However, the antibacterial test was conducted in a Streptococcus mutans (S. mutans) inoculated brain-heart infusion (BHI) medium containing each disinfectant.…”

Antibacterial activity and dentin bonding ability of combined use of Clearfil SE Protect and sodium hypochlorite

“…Due to its potent, Dijken demonstrated that acceptable bonded restorations were achieved after pretreatment of a tooth with NaOCl 14) . Since then, the benefit of NaOCl against bacteria on dentin surfaces has also been demonstrated [15][16][17] . QACs, such as cetylpyridinium chloride or benzalkonium chloride, are widely used as antibacterial agents 18) .…”