Antibacterial, antioxidant and cytotoxic activities of different fractions of acetone extract from flowers of Dipterocarpus intricatus Dyer (Dipterocarpaceae)

Abstract

This study has shown for the first time the antimicrobial, antioxidant and cytotoxicity of 3 fractions of acetone extract, including hexane, chloroform and ethyl acetate from flowers of Dipterocarpus intricatus. Antibacterial test using disc diffusion method showed that the chloroform and ethyl acetate fractions inhibited the growth of all the tested bacteria, including Escherichia coli, Pseudomonas aeruginosa, Salmonella enteritidis, Salmonella typhimurium, Bacillus cereus and Staphylococcus aureus while the hexane fraction showed the antibacterial activity against B. cereus and S. enteritidis. Antioxidant activity and cancer cell resistance of those extracts were conducted using DPPH and MTT methods respectively. As a result, the DPPH radical scavenging activity of the hexane, chloroform and ethyl acetate fractions were determined with the IC50 values of 0.508, 0.22 and 0.075 mg/mL respectively while the cytotoxicity to HepG2 cell line of those fractions was 163.3 ppm, 106.7 ppm and 459.3 ppm. These results suggested the potential application of these fractions isolated from D. intricatus flowers as the natural antimicrobial, antioxidant and cytotoxic agents for medicine.

Phlogacanthus cornutus: chemical profiles and antioxidant effects

“…The solid material was further soaked in 300 ml and 200 ml sequentially of the same solvent and filtered to collect the extracts. All the three acetone extracts were then combined and condensed on an a rotary evaporator at 40 ℃ to remove the solvent (8).…”

“…The extract was subjected to a rotary evaporator at 40 ºC to remove the solvent. The lower layer was then used to collect the ethyl acetate and chloroform (Thermo Fisher Scientific, USA) fractions using the same procedure (8).…”

Determination of a Chemical Marker in Dipterocarpus alatus Oleoresin Samples and Bioactivity Screening via Antioxidants, Nitric Oxide Inhibition on Murine RAW 264.7 Cells, and Collagen Production on Normal Human Dermal Fibroblasts

“…The absorbance was measured at 517 nm using the microplate reader. 15 The standard Trolox was used as a positive control. The inhibition percentage of the DPPH radical scavenging activity was calculated as follows with the equation below:…”

Phytochemical screening of Malaysian Dipterocarpaceae species from Kuala Keniam, Taman Negara Pahang

“…Additionally, the leaf, bark and twig extracts of Dipterocarpus alatus demonstrated significant cytotoxicity and antioxidant activity against the U937 cell lines [8]. The hexane, chloroform and ethyl acetate extracts from the flowers of D. intricatus exhibited antioxidant activity on DPPH radical, antibacterial ability against B. cereus, E. coli, P. aeruginosa, S. enteritidis, S. typhimurium and S. aureus as well as cytotoxicity on Hep G2 cell lines [9].…”