Antibacterial, antibiofilm and antiquorum sensing effects ofThymus daenensisandSatureja hortensisessential oils againstStaphylococcus aureusisolates

Sharifi, Aram; Mohammadzadeh, Abdolmajid; Salehi, Taghi Zahraei; Mahmoodi, Pezhman

doi:10.1111/jam.13639

Cited by 85 publications

(74 citation statements)

References 49 publications

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“…Interestingly, this result was confirmed in the microscope images. In S. aureus like other pathogenic bacteria, biofilm formation contributes to colonization, pathogenicity, and bacterial resistance ( Sharifi et al 2018 ). In a study performed by Dosler et al .…”

Section: Discussionmentioning

confidence: 99%

Synergistic activity of melittin with mupirocin: A study against methicillin-resistant S. Aureus (MRSA) and methicillin-susceptible S. Aureus (MSSA) isolates

Alni

Tavasoli

Barati

et al. 2020

Saudi Journal of Biological Sciences

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Methicillin-Resistant Staphylococcus aureus (MRSA) biofilms are involved in various nosocomial infections, being in the limelight of academic research. The current study aimed to determine the antimicrobial effects of melittin on planktonic and biofilm forms of S. aureus . Following the identification of MRSA and SCCmec types (using PCR method), Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and fractional inhibitory concentration index (FICi), for melittin and mupirocin were determined by broth microdilution assay. Melittin anti-biofilm activity was determined, using a microtiter-plate test (MtP) and scanning electron microscope (SEM) methods. The quorum sensing inhibitory activity of ½ MIC melittin was examined using a quantitative real-time RT-PCR method, and melittin cytotoxicity on Vero cells was examined by tetrazolium-based colorimetric (MTT) test. The Results of our study showed that Geometric means of MIC values of the melittin and mupirocin were 4.4 and 14.22 μg/ml respectively. The geometric mean of the FICi for both melittin-mupirocin was 0.75. No S. aureus biofilm was formed and hld gene (as a biofilm regulator) expression down-regulated. It seems that melittin can be useful in the treatment of S. aureus infections (especially MRSA) by reducing the hld expression. Furthermore, synergistic growth-inhibitory effects of mupirocin with melittin could be considered as a promising approach in the treatment of MRSA isolates.

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Section: Discussionmentioning

confidence: 99%

Synergistic activity of melittin with mupirocin: A study against methicillin-resistant S. Aureus (MRSA) and methicillin-susceptible S. Aureus (MSSA) isolates

Alni

Tavasoli

Barati

et al. 2020

Saudi Journal of Biological Sciences

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“…Low concentrations of tea tree oil (from M. alternifolia) containing terpinen-4-ol inhibit MRSA biofilm formation [45]. Essential oil from S. hortensis, containing carvacrol, terpinene and thymol, significantly down-regulates the S. aureus hld gene expression at concentrations below the bacterial minimal inhibitory concentration [46]. The triterpene saponin glycyrrhetinic acid isolated from liquorice is a potent inhibitor of S. aureus alpha-haemolysin (Hla) at 1-8 µg/mL, concentrations well below its minimal inhibitory concentration (MIC) of >512 µg/mL and protects mice from S. aureus pneumonia [47].…”

Section: Discussionmentioning

confidence: 99%

Rosmarinus officinalis L. (Rosemary) Extracts Containing Carnosic Acid and Carnosol are Potent Quorum Sensing Inhibitors of Staphylococcus aureus Virulence

2020

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Staphylococcus aureus is an opportunistic pathogen and a common cause of skin infection. S. aureus also plays a role in the pathogenesis of the chronic inflammatory skin disease, atopic dermatitis. S. aureus virulence involves activation of the quorum sensing agr operon. In this paper, we show that the diterpene carnosic acid, present in R. officinalis L. (rosemary) leaves, is a specific inhibitor of S. aureus agr expression as low as 5 μM. Carnosol and rosmarinic acid are two other phytochemicals present in rosemary leaves. Carnosol, but not rosmarinic acid, is also a potent agr expression inhibitor. Natural rosemary extracts containing carnosic acid and carnosol inhibit S. aureus agr expression, both in luciferase reporter strains and in wild type strains isolated from patients with atopic dermatitis. Specific inhibition of S. aureus virulence using topical formulations of rosemary extract may offer a practical approach to preventing and treating flares of atopic dermatitis.

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“…Also, it can regenerate known antioxidants such as ascorbate, tocopherol, and glutathione by converting their oxidized form to reduced form. CoQ10 increases the ratio of reduced to oxidized glutathione in the liver and decreases the levels of reactive oxygen species increases the activity of mitochondrial electron transport chain complexes, and finally lowers the effects of oxidative stress [26,27].…”

Section: Discussionmentioning

confidence: 99%

Effect of Coenzyme Q10 Supplementation on Liver Total Oxidant/Antioxidant Status in Streptozotocin-induced Diabetic Rats

Samimi

Baazm

Eftekhar

et al. 2019

J. Arak Univ. Med. Sci.

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Background and Aim Oxidative stress is the main factor in the development and progression of diabetes and its related complications. There is growing evidence that antioxidants supplementation can improve oxidative stress induced in diabetes. The present investigation was conducted to study the effects of Coenzyme Q10 (CoQ10) on the Oxidative Stress Index (OSI) in diabetic rats. Methods and Materials A total of 30 male rats were divided into five groups: saline, sesame oil (as a vehicle), CoQ10-treated (10 mg/kg/day), diabetic (induced with streptozotocin: 55 mg/kg), and CoQ10-treated diabetic (10 mg/kg/d). Then, we measured the Malondialdehyde (MDA), Total Oxidant Status (TOS), and Total Antioxidant Capacity (TAC) levels in the rats' liver homogenate. Additionally, the OSI was calculated. Ethical Considerations The Research Ethics Committee of Arak University of Medical Sciences approved this study (Code: IR.ARAKMU.REC.1397.119). Results results showed a significant decrease in the level of liver MDA (P=0.022), TOS (P=0.03), and OSI (P=0.028) in the CoQ10-treated diabetic group compared to the diabetic rats. No significant change was observed in the total thiol group (P=0.25) and TAC (P=0.77) level in diabetic rats' livers treated with CoQ10 compared to diabetic rats. Conclusion CoQ10 supplementation can reduce oxidative stress induced in the liver of diabetic rats through the suppression of TOS, OSI, and lipid peroxidation.

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Antibacterial, antibiofilm and antiquorum sensing effects ofThymus daenensisandSatureja hortensisessential oils againstStaphylococcus aureusisolates

Abstract: The present study introduced T. daenensis and S. hortensis EOs as new antibiofilm, and S. hortensis EO as anti-QS herbal agents with natural origin against Staph. aureus.

Cited by 85 publications

References 49 publications

Synergistic activity of melittin with mupirocin: A study against methicillin-resistant S. Aureus (MRSA) and methicillin-susceptible S. Aureus (MSSA) isolates

Synergistic activity of melittin with mupirocin: A study against methicillin-resistant S. Aureus (MRSA) and methicillin-susceptible S. Aureus (MSSA) isolates

Rosmarinus officinalis L. (Rosemary) Extracts Containing Carnosic Acid and Carnosol are Potent Quorum Sensing Inhibitors of Staphylococcus aureus Virulence

Effect of Coenzyme Q10 Supplementation on Liver Total Oxidant/Antioxidant Status in Streptozotocin-induced Diabetic Rats

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