Antibacterial, anti‐inflammatory and antioxidant effects of acetyl‐11‐α‐keto‐β‐boswellic acid mediated silver nanoparticles in experimental murine mastitis

Abstract

Mastitis is an important economic disease causing production losses in dairy industry. Antibiotics are becoming ineffective in controlling mastitis due to the emergence of resistant strains requiring the development of novel therapeutic agents. In this study, the authors present the phytochemical synthesis of silver nanoparticles (AgNPs) with acetyl-11-α-keto-βboswellic acid and evaluation of their activity in Staphylococcus aureus induced murine mastitis. Boswellic acid mediated AgNP (BANS) were oval, polydispersed (99.8 nm) with an minimum inhibitory concentration of 0.033 µg ml −1 against S. aureus, inhibitory concentration (IC 50) of 30.04 µg ml −1 on mouse splenocytes and safe at an in vivo acute oral dose of 3.5 mg kg −1 in mice. Mastitis was induced in lactating mice by inoculating S. aureus (log 10 5.60 cfu) and treated 6 h post-inoculation with BANS (0.12 mg kg −1 , intramammary and intraperitoneal), and cefepime (1 mg kg −1 , intraperitoneal). S. aureus inoculated mice showed increased bacterial load, neutrophil infiltration in mammary glands and elevated C-reactive protein (CRP) in serum. Oxidative stress was also observed with elevated malondialdehyde level, superoxide dismutase (SOD) and catalase (CAT) activities. BANS treatment significantly (P < 0.05) reduced bacterial load, CRP, SOD, CAT activities and neutrophil infiltration in affected mammary glands. BANS could be a potential therapeutic agent for managing bovine mastitis.

<p>Acetyl-11-keto-β-boswellic Acid Inhibits Precancerous Breast Lesion MCF-10AT Cells via Regulation of LINC00707/miR-206 that Reduces Estrogen Receptor-α</p>

“…8,9 AKBA, a pentacyclic terpenoid, is the active component of frankincense and has been exploited for various medicinal applications, such as antibacterial, anti-inflammatory, analgesic, anti-oxidant. 10,11 Recently, AKBA was also found to exhibit antitumor effects in human cell lines established from prostate cancer, breast cancer and glioblastoma. [12][13][14] Csuk et al demonstrated that boswellic acid and its derivatives induce apoptosis in breast and cervical cancers cells.…”

Ethnoveterinary Uses of Certain Yemeni Plants: A Review of the Scientific Evidence

“… Acetyl-11- α -keto- β -boswellic acid -mediated silver nanoparticles showed superior antibacterial, anti-inflammatory, and antioxidant properties than cefepime in mastitis-induced mammary glands in mice 55 . …”

“…S. aureus is one of the leading causes of mastitis, resulting in an annual economic loss of $2 billion globally. S. aureus is also responsible for the emergence of resistant organisms and antibiotic residues in dairy products 55 . Acetyl-11- α -keto- β -boswellic acid-mediated silver nanoparticles (BANS, 0.12 mg · kg −1 , intramammary and intraperitoneal) were reported to exert superior antibacterial (36%), anti-inflammatory (95%), and antioxidant effects compared with the antibiotic cefepime (1 mg · kg −1 , intraperitoneal) in S. aureus -induced murine mastitis.…”

“…Acetyl-11-α-keto-β-boswellic acid-mediated silver nanoparticles (BANS, 0.12 mg • kg −1 , intramammary and intraperitoneal) were reported to exert superior antibacterial (36 %), antiinflammatory (95 %), and antioxidant effects compared with the antibiotic cefepime (1 mg • kg −1 , intraperitoneal) in S. aureus-induced murine mastitis. BANS treatment significantly (p < 0.05) reduced bacterial load, C-reactive protein, superoxide dismutase, catalase activities, and neutrophil infiltration in affected mammary glands [55]. The antibacterial, anti-inflammatory, and antioxidant effects of acetyl-11-α-keto-β-boswellic acid could lend some support for the local use of oleo-gum resin of B. sacra, which may be able to target pathogens such as S. aureus present at the teat opening and thus help to prevent bacteria that cause mastitis from entering the teat canal and colonizing the mammary tissue, as well as reduce the inflammation of the udder surface.…”

“…2 Cl 2 extracts of the crude methanol extract) obtained from Omani B. sacra oleo-gum resin: ▪ Inhibition of superoxide anion (> 50 %), while the CH 2 Cl 2 fraction and the sub-fraction of 40 % CH 2 Cl 2 /n-hexane fraction, as well as essential oil of Shabi frankincense grade, showed moderate inhibitory activity of DPPH free radicals [54].α-Pinene▪ Moderate DPPH, superoxide anion radicals scavenging activity, and reducing power activity[46].Antibacterial, anti-inflammatory, and antioxidant ▪ Acetyl-11-α-keto-β-boswellic acid-mediated silver nanoparticles showed superior antibacterial, anti-inflammatory, and antioxidant properties than cefepime in mastitis-induced mammary glands in mice[55].Soqotraen Boswellia species (such as B. ameero Balf.f. ; B. bullata Thulin; B. dioscoridis Thulin; B. elongate Balf.f.…”