Antibacterial and Phytochemical Screening of Artemisia Species

Bordean, Maria-Evelina; Ungur, Rodica; Țoc, Dan Alexandru; Borda, Ileana Monica; (Petruț), Georgiana Smaranda Martiș; Pop, Carmen; Filip, M; Vlassa, M.; Năsui, Bogdana Adriana; Pop, Anamaria; Cintezã, Delia; Popa, Florina Ligia; Marian, Sabina; Szanto, Lidia; Muste, Sevastița

doi:10.3390/antiox12030596

Cited by 22 publications

(12 citation statements)

References 84 publications

(102 reference statements)

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“…The antimicrobial properties of the A. annua extract prepared from cv. Novichok (the Nikitsky Botanic Garden) were comparable to those of the A. annua methanol extracts from India, but they were lower than those of the A. annua methanol extracts from Bulgaria [ 85 , 86 ] and significantly higher than those of the ethanol extracts from the plants grown in Romania [ 87 ]. Among all the species under study, the A. dracunculus ethanol extract demonstrated a selective antimicrobial activity against R. iranicus , requiring the lowest inhibitory and bactericidal concentrations.…”

Section: Resultsmentioning

confidence: 99%

Five Different Artemisia L. Species Ethanol Extracts’ Phytochemical Composition and Their Antimicrobial and Nematocide Activity

Nikitin,

Fitsev,

Egorova

et al. 2023

IJMS

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Among the plants that exhibit significant or established pharmacological activity, the genus Artemisia L. deserves special attention. This genus comprises over 500 species belonging to the largest Asteraceae family. Our study aimed at providing a comprehensive evaluation of the phytochemical composition of the ethanol extracts of five different Artemisia L. species (collected from the southwest of the Russian Federation) and their antimicrobial and nematocide activity as follows: A. annua cv. Novichok., A. dracunculus cv. Smaragd, A. santonica cv. Citral, A. abrotanum cv. Euxin, and A. scoparia cv. Tavrida. The study of the ethanol extracts of the five different Artemisia L. species using the methods of gas chromatography–mass spectrometry (GC–MS) and high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (HPLC–MS/MS) allowed establishing their phytochemical profile. The obtained data on the of five different Artemisia L. species ethanol extracts’ phytochemical composition were used to predict the antibacterial and antifungal activity against phytopathogenic microorganisms and nematocidal activity against the free-living soil nematode Caenorhabditis elegans. The major compounds found in the composition of the Artemisia L. ethanol extracts were monoterpenes, sesquiterpenes, flavonoids, flavonoid glycosides, coumarins, and phenolic acids. The antibacterial and antifungal activity of the extracts began to manifest at a concentration of 150 µg/mL. The A. dracunculus cv. Smaragd extract had a selective effect against Gram-positive R. iranicus and B. subtilis bacteria, whereas the A. scoparia cv. Tavrida extract had a selective effect against Gram-negative A. tumefaciens and X. arboricola bacteria and A. solani, R. solani and F. graminearum fungi. The A. annua cv. Novichok, A. dracunculus cv. Smaragd, and A. santonica cv. Citral extracts in the concentration range of 31.3–1000 µg/mL caused the death of nematodes. It was established that A. annua cv. Novichok affects the UNC-63 protein, the molecular target of which is the nicotine receptor of the N-subtype.

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Section: Resultsmentioning

confidence: 99%

Five Different Artemisia L. Species Ethanol Extracts’ Phytochemical Composition and Their Antimicrobial and Nematocide Activity

Nikitin,

Fitsev,

Egorova

et al. 2023

IJMS

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“…On the other hand, Artemisia has shown antibacterial activity against drug-resistant bacterial strains. For example, Artemisia absinthium L. leaf extracts exhibit antibacterial activity against S. aureus [141]. Given the results of our review, we believe that the limited nature of the direct evidence on the antimicrobial activities of Vitex species, when compared to other genera, is due to the fact that Vitex species have been less extensively studied for their antimicrobial properties, and more research is needed to understand their full potential.…”

Section: Discussionmentioning

confidence: 97%

Vitex Genus as a Source of Antimicrobial Agents

Islam,

Caldeira,

Caniça

et al. 2024

Plants

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Vitex L. is the largest genus of the Lamiaceae family, and most of its species are used in the traditional medicinal systems of different countries. A systematic review was conducted, according to the PRISMA methodology, to determine the potential of Vitex plants as sources of antimicrobial agents, resulting in 2610 scientific publications from which 141 articles were selected. Data analysis confirmed that Vitex species are used in traditional medicine for symptoms of possible infectious diseases. Conducted studies showed that these medicinal plants exhibited in vitro antimicrobial activity against Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Vitex agnus-castus L. and Vitex negundo L. have been the most studied species, not only against bacterial strains but also against fungi such as Aspergillus niger and Candida albicans, viruses such as HIV-1, and parasites such as Plasmodium falciparum. Natural products like agnucastoside, negundol, negundoside, and vitegnoside have been identified in Vitex extracts and their antimicrobial activity against a wide range of microbial strains has been determined. Negundoside showed significant antimicrobial activity against Staphylococcus aureus (MIC 12.5 µg/mL). Our results show that Vitex species are potential sources of new natural antimicrobial agents. However, further experimental studies need to be conducted.

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“…A previous study showed that the leaf and stem ethanolic extracts of A. absinthium and A. annua showed significant antibacterial activity against carbapenem-resistant Klebsiella spp., extended-spectrum β-lactamases E. coli (ESBL) and ESBL Klebsiella spp. ( Bordean et al, 2023 ). In our study, the highest antibacterial activity (MIC = 47.5 μg/mL, MBC = 95 μg/mL, IZ = 8 ± 1 mm and 50.5% inhibition of biofilm at 2 MIC concentration) was recorded for WE against S. typhimurium .…”

Section: Discussionmentioning

confidence: 99%

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

Rahmani,

Karimi,

Saffari

et al. 2024

Front. Chem.

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Introduction: Nanoemulsion and nanoencapsulation are attractive novel methods that can be used for incorporating active plant extracts in food preparations and pharmaceutical formulations. In the current study, we aimed to investigate the anticancer and antibacterial effects of hydroethanolic extracts of Nettle (NE), Wormwood (WE), and the combination of the two plants (CNWE), as well as their nanoemulsion forms (NN, NW, CNNW) and nanoencapsulation forms (CN, CW, and CCNW).Methods: The morphology and structure of the nanoemulsion and nanoencapsulation preparations were assessed utilizing dynamic light scattering (DLS) along with transmission electron microscopy (TEM). The antibacterial activity of the prepared formulations were assessed by determining minimum inhibitory concentration (MIC), zone of inhibition diameter, minimum bactericidal concentration (MBC), along with biofilm growth inhibition against Salmonaella typhimurium and Klebsiella. pneumoniae. The anticancer activity was evaluated via a MTT assay in the colon cancer cell line (HCT116).Results: The nanoemulsion and nanoencapsulation particle size varied between 10 and 50 nm and 60 and 110 nm, respectively. The MIC values were between 11.25 and 95 µg/mL along with MBC values between 11.25 and 190 µg/mL. The highest inhibition of biofilm formation was observed with CCNW against K. pneumoniae (∼78.5%) and S. typhimurium (∼73%). In descending order, the inhibition of biofilm formation was CCNW > CW > CN > CNNW > NN > NW > CNWE > NE > WE against the tested bacteria. The IC50 values for NE, WE, CNWE, NN, NW, CNNW, CN, CW, and CCNW were determined as 250, 170, 560, 380, 312, 370, 250, 420, and 700 µg/mL, respectively. Exposure to a high concentration of NW resulted in a significantly lower HCT116 viability compared to other groups. Taken together, CNNW, and CCNW showed the highest antibacterial and anticancer activitiy.Discussion: Nanoemulsion and nanoencapsulation were effective ways to increase the antibacterial and anticancer activity of the extracts and could be used in the food and pharmaceutical industries.

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Antibacterial and Phytochemical Screening of Artemisia Species

Cited by 22 publications

References 84 publications

Five Different Artemisia L. Species Ethanol Extracts’ Phytochemical Composition and Their Antimicrobial and Nematocide Activity

Five Different Artemisia L. Species Ethanol Extracts’ Phytochemical Composition and Their Antimicrobial and Nematocide Activity

Vitex Genus as a Source of Antimicrobial Agents

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

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