“…It was generally accepted that Cu 2+ could be the active species for copper oxide antibacterial materials, responsible for the antibacterial activity . This is because the Cu 2+ ions released from copper oxides may produce hazardous effects by generating the reactive oxygen species (ROS), for instance O 2 2–• , • OH, and HO 2 –• , which damage the cytomembrane and then disrupt amino acid synthesis and DNA. ,, The ROS could be generated from the surface defect sites in the Cu 4 O 3 microstructure or induced by the high concentrations of free Cu 2+ ions released from the Cu 4 O 3 microstructure. ,, Figure B,C confirms that the concentration of the bacteria is closely related with the concentration of Cu 2+ ions. With increasing quantity of the Cu 4 O 3 from 2 to 8 mg, the concentrations of Cu 2+ ions increase near linearly; meanwhile, the concentrations of S. aureus and E. coli decrease near linearly until the critical values, from which the concentrations of the bacteria drop sharply to very low values.…”