Antibacterial and cytotoxicity activities of biosynthesized silver oxide (Ag2O) nanoparticles using Bacillus paramycoides

Dharmaraj, Divya; Krishnamoorthy, M.; Rajendran, Kumar; Kannan, K.; Annamalai, Jeyameenakshi; Durairaj, Karthick Rajan; Prakash, S.; Ethiraj, Kannapiran

doi:10.1016/j.jddst.2020.102111

Cited by 53 publications

(34 citation statements)

References 47 publications

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“…The size distribution histogram revealed that the average size of the nanoparticles, which ranged in size from 30 to 47 nm, was 32.7 nm ( Figure 6 A), which was smaller than that formed with B. thuringiensis (43.52–142.97 nm) [ 73 ]. The results are consistent with other studies that have been published and show that silver oxide nanoparticles produced using B. linens varied in size from 25 to 70 nm [ 74 ]. Additionally, the size of spherical AgNPs synthesized by Bacillus sp KFU36 was extended between 5 and 15 nm [ 42 ].…”

Section: Resultssupporting

confidence: 93%

“…AgNPs synthesized by B. subtilis (P3) had an average particle size of 805 nm [ 43 ], and this significant disparity could be related to sample variability and synthesis method. A DLS study of silver oxide (Ag 2 O) nanoparticles produced by B. paramycoides revealed that the majority of the particles are between 50 and 150 nm in size [ 74 ]. According to Saied et al [ 34 ], the average diameter of the AgNPs generated by Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

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Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC50 = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis.

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Section: Resultssupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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“…In the resulting FTIR spectrum (Figure 5) several absorption bands are presented, which correspond to the functional groups of the biomolecules existing in the plant extract of H. hirsuta. Seven main absorption peaks were observed, the broad peak centered at 3400 and 3533 cm −1 are assigned to the O−H stretching vibrations of flavonoids and alkaloids, the intense peak at 1123 and 1687 cm −1 are due to the C=O stretching and N−H bending vibrations of the primary amide group which often exist in proteins [34]. The two peaks located at 1392 cm −1 pharmaceuticals and medicine and 1621 cm −1 are attributed to Nitro-banding NO and COC stretching vibrations of the aromatic ring respectively [35].…”

Section: Fourier Transform Infrared Spectroscopy (Ftir)mentioning

confidence: 99%

A New Approach for the Green Biosynthesis of Silver Oxide Nanoparticles Ag2O, Characterization and Catalytic Application

El-Ghmari

Farah

Ech‐Chahad

2021

Bull. Chem. React. Eng. Catal.

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In this paper, a facile and green approach for the synthesis of silver oxide nanoparticles Ag2O NPs was performed using the extract of the wild plant Herniaria hirsuta (H. hirsuta). Different spectral methods were used for the characterization of the biosynthesized Ag2O NPs, ultraviolet-visible (UV-Vis) spectroscopy gave a surface plasmon resonance (SPR) peak of Ag2O NPs is 430 nm, estimation of direct and indirect forbidden gap bands are respectively 3.76 eV and 3.68 eV; Fourier transform infrared (FTIR) spectral analysis revealed the groups responsible for the stability and synthesis of Ag2O NPs. The morphology of Ag2O NPs was studied by scanning electron microscopy (SEM) showing a nearly spherical shape of Ag2O NPs, and X-ray diffraction (XRD) study confirmed the crystallinity of Ag2O NPs with a crystallinity size of 15.51 nm. The catalytic activity of Ag2O NPs, as well as the rings number were studied by the degradation of methylene blue dye. Copyright © 2021 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0).

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“…Their use is not only limited to health applications but also extends to the food and textile industries [31]. Consequently, following the SARS-CoV-2 pandemic, silver and silver oxide have been subjected to various synthesis studies, analyzing their antimicrobial features [32], cytotoxicity activities [33] and bioactive properties [34].…”

Section: Introductionmentioning

confidence: 99%

Facile Route to Effective Antimicrobial Aluminum Oxide Layer Realized by Co-Deposition with Silver Nitrate

et al. 2021

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The emergence and spreading of the SARS-CoV-2 pandemic has forced the focus of attention on a significant issue: the realization of antimicrobial surfaces for public spaces, which do not require extensive use of disinfectants. Silver represents one of the most used elements in this context, thanks to its excellent biocidal performance. This work describes a simple method for the realization of anodized aluminum layers, whose antimicrobial features are ensured by the co-deposition with silver nitrate. The durability and the chemical resistance of the samples were evaluated by means of several accelerated degradation tests, such as the exposure in a salt spray chamber, the contact with synthetic sweat and the scrub test, highlighting the residual influence of silver in altering the protective behavior of the alumina layers. Furthermore, the ISO 22196:2011 standard was used as the reference protocol to set up an assay to measure the effective antibacterial activity of the alumina-Ag layers against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, even at low concentrations of silver. Finally, the Ag-containing aluminum oxide layers exhibited excellent antimicrobial performances also following the chemical–physical degradation processes, ensuring good durability over time of the antimicrobial surfaces. Overall, this work introduces a simple route for the realization of anodized aluminum surfaces with excellent antibacterial properties.

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Antibacterial and cytotoxicity activities of biosynthesized silver oxide (Ag2O) nanoparticles using Bacillus paramycoides

Cited by 53 publications

References 47 publications

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

A New Approach for the Green Biosynthesis of Silver Oxide Nanoparticles Ag2O, Characterization and Catalytic Application

Facile Route to Effective Antimicrobial Aluminum Oxide Layer Realized by Co-Deposition with Silver Nitrate

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