Antibacterial and cytotoxic effect of honey mediated copper nanoparticles synthesized using ultrasonic assistance

Abstract

In this study, a comparative study of effect using honey on copper nanoparticles (Cu-NPs) via simple, environmentally friendly process and inexpensive route was reported. Honey and ascorbic acid act as stabilizing and reducing agents with the assistance of sonochemical method. The products were characterized using UV-visible (UV-vis) spectroscopy, X-Ray Diffraction (XRD), High-Resolution Transmission Electron Microscopy (HRTEM), Field-Emission Scanning Electron Microscopy (FESEM) and Fourier Transform Infrared (FTIR) spectroscopy. The reddish brown colour demonstrated the formation of Cu-NPs and UV-visible proved the plasmon resonance of Cu-NPs. XRD also confirmed a highly pure Cu-NPs obtained with absence of copper oxide in which the structure is crystalline. The spherical size of the Cu-NPs was acquire in the presence of honey which is 3.68 ± 0.78 nm with narrow particle distribution. The antibacterial activity was seen against gram-positive and gram-negative bacteria which are Enterococcus faecalis (E. faecalis) and Escherichia coli (E. coli). At higher concentration of Cu-NPs, they were more effective in killing both bacteria. The Cu-NPs without and with honey exhibited toxicities toward normal and cancerous cells. However, Cu-NPs without honey showed more potent killing activity against normal and cancer cells.

Bio-Mediated Synthesis and Characterisation of Silver Nanocarrier, and Its Potent Anticancer Action

“…This method utilises bio-reagents such as bacteria, fungi, enzymes, and plants as both reducing and stabilizing agents [8,9,10]. Amongst all, plants-mediated synthesis is preferred as the morphology of resulting NPs can be easily manipulated by varying different parameters [11,12,13,14]. Each part of plants can be utilised for NPs synthesis, leaves being the most common part as compared to others such as bark, fruit, seed, flower, root and peel [15].…”

“…In the past, 60 types of xanthones had been isolated from the GM pericarp [17]. Similarly, GM has also been associated with other pharmacological actions such as antitumor, antibacterial, antifungal and antiviral activities [12,13,14,18,19]. Mechanisms of anticancer xanthones have previously been revealed, including induction of apoptosis through regular cell death pathways, suppression of cell proliferation, and inhibition of metastasis by blocking the anti-apoptotic molecules and cell cycle arrest [20].…”

Algae-mediated route to biogenic cuprous oxide nanoparticles and spindle-like CaCO3: a comparative study, facile synthesis, and biological properties

“…Our results are in agreement with recent studies which have approved cytotoxicity of metal nanoparticles. 61,[68][69][70] Present study exhibits high potentiality of Cu 2 O for its anticancer activity as conrmed by concentration dependent cytotoxicity of Cu 2 O nanoparticles on K562 cells in addition to lower toxicity to normal cells. However, several researches are underway to investigate the mechanism of Cu 2 O action and possible pathways of cytotoxicity and their side effects.…”

Green Synthesized Montmorillonite/Carrageenan/Fe3O4 Nanocomposites for pH-Responsive Release of Protocatechuic Acid and Its Anticancer Activity

“…MTS assay was performed as previously described [ 59 , 61 , 62 , 63 ] to evaluate the cellular killing effect of NCs. For both HCT116 and CCD112, 5,000 cells per well (100 µL/well) were seeded onto 96-well plates and incubated overnight in a 5% CO 2 incubator at 37 °C for complete adherence.…”

“…In addition, inhibitory concentration causing 50% growth inhibition (IC 50 ) was determined using an online calculator (https://www.aatbio.com/tools/ic50-calculator) as previously described [59,[61][62][63].…”