Antibacterial and antioxidant potential of ethyl acetate extract from Streptomyces AIA12 and AIA17 isolated from gut of Chanos chanos

Abstract

Abstract. Kurnianto MA, Kusumaningrum HD, Lioe HN, Chasanah E. 2021. Antibacterial and antioxidant potential of ethyl acetate extract from Streptomyces AIA12 and AIA17 isolated from gut of Chanos chanos. Biodiversitas 22: 3196-3206. Streptomyces has been recognized as a promising and productive source of antibacterial and antioxidant compounds. The phenotypic and genomic characterizations indicated AIA12 and AIA17 are closely related to the S. globisporus ARGB01 and S. misionensis S1-SC15, respectively. Incubation on yeast-malt extract broth for nine days (AIA12) and 11 days (AIA17) proved to be the best growth medium and optimum antibacterial production time for these two strains. Crude-extract of extracellular secondary metabolites, obtained by ethyl acetate extraction, demonstrated broad-spectrum inhibitory activity against Pseudomonas aeruginosa InaCC B52, Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 14028, and Listeria monocytogenes with minimum inhibitory and minimum bactericidal concentrations from 2.5 to 0.31 and 5.0 to 0.31 mg mL-1, respectively. Evaluation of antioxidant showed AIA17 crude-extract had moderate DPPH scavenging and antioxidant activities of 65.122% ± 0.56 and 28.178 ± 0.24 mg ascorbic acid equivalent antioxidant capacity (AEAC g-1), respectively. The identification of compounds through profiling with RP-HPLC showed optimum absorbance at 210 and 214 nm, which showed the presence of peptide groups in the constituent compounds' molecular structure. These findings indicate that Chanos chanos-derived Streptomyces produces valuable bioactive compounds with various promising biological activities.

Partial Purification and Characterization of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from the Gut of Chanos chanos

“…ANOVA followed by Duncan's multiple range test indicated that the growth absorbance of test bacteria exposed to CFS was significantly lower than that of test bacteria not exposed to CFS. This result indicates that the presence of extracellular metabolites produced by Streptomyces in CFS could inhibit the growth of the test bacteria and supports previous findings demonstrating the potential antibacterial properties of metabolites produced by Streptomyces isolated from the gut of C. chanos [ 23 , 24 ]. The difference in antibacterial activity produced is due to differences in the concentration of extracellular metabolites produced [ 13 ].…”

“…Six Streptomyces (i.e., S. variabilis SCA-5, S. variabilis SCA-11, S. variabilis AIA-10, S. labedae SCA-8, S. globisporus AIA-12, and S. misionensis AIA-17) were obtained from the gut of milkfish ( C. chanos ) [ 23 , 24 ]. The strains were identified through their morphological, physiological, biochemical, and molecular characteristics by 16S rRNA gene sequencing.…”

“…The six Streptomyces isolates were inoculated on ISP-2 and incubated at 30°C during the optimum antibacterial production time (9–11 days) with agitation in a shaker incubator (New Brunswick, Germany) [ 24 ]. Bacterial cultures were centrifuged (Hermle, Germany) at 7,000 × g and 4°C for 15 min to separate the supernatant from the cell biomass.…”

“…Several studies have discovered metabolites with potential bioactivity produced by Streptomyces associated with fish [ 20 , 22 , 23 ]. Kurnianto et al [ 23 , 24 ], for instance, identified a suspected peptide-containing antibacterial metabolite with broad spectrum antibacterial activity produced by Streptomyces associated with Chanos chanos .…”

Purification, HR-LC-ESI-MS-MS Identification, and Peptide Prediction of Bacteriocin-Like Inhibitory Substances Produced by Streptomyces sp. Isolated from Chanos chanos

“…BLIS produced by six Streptomyces isolated from the gut of Chanos chanos: S. variabilis SCA5 (S5), S. variabilis SCA11 (S11), S. variabilis AIA10 (A10), S. labedae SCA8 (S8), S. globisporus AIA12 (A12) and S. misionensis AIA17 (A17) [15][16][17] with the method and culture conditions according to Kurnianto et al [18]. BLIS in freeze-dried form dissolved in distilled water.…”