Antibacterial and antifungal activity of crude and freeze-dried bacteriocin-like inhibitory substance produced by Pediococcus pentosaceus

Abstract

Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS). BLIS inhibited the growth of spoilage bacteria belonging to Lactobacillus, Enterococcus and Listeria genera with higher activity than Nisaplin used as control. It gave rise to inhibition halos with diameters from 9.70 to 20.00 mm, with Lactobacillus sakei being the most sensitive strain (13.50-20.00 mm). It also effectively suppressed the growth of fungi isolated from corn grain silage for up to 25 days and impaired morphology of colonies by likely affecting fungal membranes. these results point out that P. pentosaceus BLIS may be used as a new promising alternative to conventional antibacterial and antifungal substances, with potential applications in agriculture and food industry as a natural bio-controlling agent. Moreover, cytotoxicity and cell death induction tests demonstrated cytotoxicity and toxicity of BLiS to human colon adenocarcinoma caco-2cells but not to peripheral blood mononuclear cells, with suggests possible applications of BLIS also in medical-pharmaceutical applications. Lactic acid bacteria (LAB) are a group of well-distributed microorganisms in nature 1-3 having lactic acid as the major product of sugar fermentation 4,5. They are Gram-positive, non-pathogenic, non-sporulating, facultative anaerobic, catalase negative and acid tolerant bacteria with a strictly fermentative metabolism 3,6 , which are often used as industrial starter cultures in food fermentation technology 7,8. Their metabolic products are recognized as Generally Regarded as Safe (GRAS) by the Food and Drug Administration (FDA) and recommended by the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA) 9. This means that they can be safely used to preserve foods 10,11 , hence offering important benefits to the food industry 12. From the technology point of view, the most important genera of LAB are Aerococcus, Carnobacterium,

Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B1, Ochratoxin A, and Zearalenone in vitro

“…Strains of Lacticaseibacillus casei and Lactobacillus acidophilus have also shown to have inhibition effects against A. parasiticus ( Abbaszadeh et al, 2015 ). LAB have been reported to produce compounds during growth with potent antifungal effects which may damage the fungal membrane and cell wall structure, resulting in lysis of hyphae and spores ( de Souza de Azevedo et al, 2020 ; Li et al, 2021 ). As indicated by the two first assays, L. brevis 2QB422 was the most effective strain in suppressing the growth of A. parasiticus , which reached a maximum diameter of 4.91 ± 0.6 cm ( Table 3 ; Figure 1 ), followed by the range of 5.26–5.46 cm for strains 3QB361 ( Lactiplantibacillus plantarum ), 3QB398 ( Levilactobacillus spp.…”

Pediococcus pentosaceus: Screening and Application as Probiotics in Food Processing

“…Antibacterial activity is an important indicator that reflects the ability of probiotics to competitively kill or suppress harmful microorganisms in the intestinal tract ( de Azevedo et al, 2020a ). Pseudomonas aeruginosa , Escherichia coli , Staphylococcus aureus , and Salmonella , etc.…”

In Vitro Assessment of Antistaphylococci, Antitumor, Immunological and Structural Characterization of Acidic Bioactive Exopolysaccharides from Marine Bacillus cereus Isolated from Saudi Arabia

“…Drug delivery, medicinal coatings, surgical sealants, and anticancer activities are just a few of the medical and industrial applications for EPS [6]. Aerococcus, Carnobacterium, Enterococcus, Lactobacillus, Lactococcus, Leuconostoc, Oenococcus, Pediococcus, Streptococcus, and Weissella, were reported to leave a significant impact in rapid industrialization [7].…”