Antibacterial and Anticancer Effects of Silver Nanoparticles Synthesised using Eragrostis tef and Vitellaria paradoxa Seeds Extract

Abstract

The present study aims to investigate the biosynthesis of silver nanoparticles using the aqueous extract of the Eragrostis tef (teff or annual bunch grass) and Vitellaria paradoxa (Shea Butter Tree) seeds extract and investigate their antimicrobial and anticancer activities. In order to synthesise the silver nanoparticles, aqueous extract of the plant was prepared and introduced into a 1mM silver nitrate solution. The synthesised AgNPs were characterised using various instrumental techniques including ultraviolet-visible spectroscopy, Fourier transformed infrared spectroscopy (FTIR), scanning electron microscope (SEM). Surface Plasmon Resonance (SPR) for AgNPs using E. tef and V. paradoxa seeds extract was observed at 40 nm. The synthesised AgNPs of E. tef and V. paradoxa was found with diameter of 12.59- 34.60 nm, 29.05- 83.94 nm respectively. The antibacterial result against the experimental pathogens was found to range from 12-22 mm and from 13-19 mm using AgNPs of E. tef, V. paradoxa seed extracts respectively. In addition the result show that there was clear effect on Acinetobacter baumannii from hospital sewage isolates. Toxic effect of AgNPs against cancer cell were 77.12 (concentration of AgNPs was 75%) of E. tef and 77.23 (concentration of AgNPs was 50%) of V. paradoxa.

Food Additive Mediated Biosynthesis of AgNPs with Antimicrobial Activity Against Hypermucoviscous Enterotoxigenic Foodborne Klebsiella pneumoniae

“…Tawfeeq et al (2017) synthesized the AgNPs from banana peels using microwaves, this biological agent played the role of reduction, stabilizing and capping. The first indication of the AgNPs formation in the biosynthesis vessel is the colour change to a dark brown colour, The darkness of the brown colour in the biosynthesis vessel is dependent on the plant extract concentration (Almudhafar & Al-Hamdani, 2022).…”

Synthesis of silver nanoparticles from coffee parasite leaf extract (Scurrulla ferruginea (Roxb. ex Jack) Danser) and potential activity as an antioxidant and anticancer

“…In silver nanoparticle synthesis, excessive chemicals are required which can cause environmental pollution, and require large costs to manufacture. Therefore, the synthesis of nanoparticles using plant extracts is an environmentally friendly approach (Xu et al, 2006;Lestari et al, 2019;Begum et al, 2020;Ijaz et al, 2020;Almudhafar et al, 2022). Compounds found in plants that function as reducing agents in nanoparticle synthesis are terpenoids, phenolics, flavonoids, tannins, steroids, saponins, alkaloids and others (Yusof et al, 2018;Lestari et al, 2019).…”

Prevalence of Antibiotic Resistance in Pediatric Malignancy Patients: A Two-Year Retrospective Study in Basra City

“…A careful understanding of the local positive blood cultures and their sensitivity and resistance profile is thus required for the development of effective treatment and doable techniques to treat bloodstream infections. Serious infections are frequently treated with beta-lactams as the first line of defense, then carbapenems frequently seen as the final resort (8) .…”