Antibacterial and antibiofilm effect of silver and gold nanoparticles in Uropathogenic Escherichia coli

Abstract

Nosocomial infections of the urinary tract are the most common nosocomial infections. Infectious diseases currently pose a life threatening due to the increasing phenomenon of infectious agent resistance. One of the factors known to cause resistance to broad-spectrum antimicrobials agent is the susceptibility to biofilm formation. The use of silver nanoparticles (NPs) is now regarded as one of the most promising strategies to target infections associated with biofilms. This study aims to determine the application of nanotechnology in the form of metal nanoparticles in targeting the formation of biofilms on infectious agents. The antibacterial and antibiofilm activity test was performed on a 96-well microtiter plate on uropathogenic Escherichia coli (UPEC) at concentrations of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 ppm. The results showed the addition of silver and gold nanoparticles at different concentrations affected and inhibited the growth of UPEC and prevented the formation of biofilms, as indicated by a decrease in the OD value. In conclusion, silver and gold nanoparticles have antibacterial and antibiofilm effects on UPEC.

Antibacterial and hemocompatibility potentials of nano-gold-cored alginate preparation against anaerobic bacteria from acne vulgaris

“…The antimicrobial potentials of GANP were dependent upon the perturbations that occurred in different bacterial components and the biofilm. All biofilm penetration methods involved hydrophobic interaction, hydrogen bonding, electrostatic, and van der Waals attraction-based interactions 61 , 62 . The current results were in agreement with previous result 63 wherein it was exhibited that the AuNPs capping by Arabic Gum exhibited a noteworthy anti-adhesion activity against Cutibacterim acne.…”

Metal-Based Nanoparticles as Antimicrobial Agents: A Review

“…Similarly, through electrostatic interactions, AuNPs cause mechanical damage to cell membranes. Furthermore, the release of metal ions from AuNPs can induce ROS generation and damage cell structure, function, and proteins . In addition to adhesion, another fundamental process is quorum sensing (QS).…”

“…Furthermore, the release of metal ions from AuNPs can induce ROS generation and damage cell structure, function, and proteins. 100 In addition to adhesion, another fundamental process is quorum sensing (QS). Several studies have shown that metal NPs can disrupt the production of QS molecules, especially self-inducers (AI-2).…”

Analysis of antibiofilm activity gold and silver nanoparticles against uropathogenic Escherichia coli using confocal laser scanning microscopy

“…A 5 mL culture was prepared in a 24-well microplate for the specified treatment, comprising 3 mL of liquid RPMI media, a glass coverslip enveloped with biofilm, 1 mL of gold nanoparticle solution (E), and 1 mL of silver nanoparticle solution (P) at concentrations of 25, 50, 75, and 100 ppm. The chosen concentrations were based on findings from prior research by Purbowati et al (2022), highlighting varied antibiofilm activity via the crystal violet method. This culture was incubated at 37 ºC for 24 h under aerobic conditions.…”