Antibacterial and Antibiofilm Effect of Unifloral Honeys against Bacteria Isolated from Chronic Wound Infections

Balázs, Viktória Lilla; Nagy-Radványi, Lilla; Bencsik-Kerekes, Erika; Koloh, Regina; Szabó, Dina; Kocsis, Béla; Kocsis, Marianna; Farkas, Ágnes

doi:10.3390/microorganisms11020509

Cited by 15 publications

(11 citation statements)

References 64 publications

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“…Among unifloral honeys, which are derived predominantly from a certain plant species or genus, the amber to dark brown, somewhat bitter-tasting chestnut honey proved to be superior in terms of antibacterial activity in comparison to other honey types from Portugal [ 16 ], Spain [ 17 , 18 ], and Turkey [ 19 ]. Previous tests by our research group also confirmed that chestnut honey had the highest antibacterial effect, compared to a set of varietal honeys harvested in Hungary, including lavender, linden, acacia, sunflower, milkweed, and goldenrod honeys [ 20 ].…”

Section: Introductionsupporting

confidence: 71%

“…In order to visualize our results, we took SEM images of P. aeruginosa -MRSA untreated samples, as well as samples treated with different honey solutions (in the case of the P. aeruginosa -MRSA and P. aeruginosa - S. epidermidis combinations: 6% honey solution, S. epidermidis -MRSA: 4% honey solution), based on the description of Balázs et al, 2023 [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

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Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity

Koloh,

Balázs,

Nagy-Radványi

et al. 2024

Antibiotics

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The irresponsible overuse of antibiotics has increased the occurrence of resistant bacterial strains, which represents one of the biggest patient safety risks today. Due to antibiotic resistance and biofilm formation in bacteria, it is becoming increasingly difficult to suppress the bacterial strains responsible for various chronic infections. Honey was proven to inhibit bacterial growth and biofilm development, offering an alternative solution in the treatment of resistant infections and chronic wounds. Our studies included chestnut honey, valued for its high antibacterial activity, and the bacteria Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, and S. epidermidis, known to form multi-species biofilm communities. Minimum inhibitory concentrations (MIC) of chestnut honey were determined for each bacterial strain. Afterwards, the mixed bacterial biofilms were treated with chestnut honey at different stages of maturity (incubation times: 2, 4, 6, 12, 24 h). The extent of biofilm inhibition was measured with a crystal violet assay and demonstrated by scanning electron microscopy (SEM). As the incubation time increased and the biofilm became more mature, inhibition rates decreased gradually. The most sensitive biofilm was the combination MRSA-S. epidermidis, with a 93.5% inhibition rate after 2 h of incubation. Our results revealed that chestnut honey is suitable for suppressing the initial and moderately mature stages of mixed biofilms.

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Section: Introductionsupporting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity

Koloh,

Balázs,

Nagy-Radványi

et al. 2024

Antibiotics

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“…The high sugar content and viscosity of honey have been shown to play a crucial role in inhibiting microbial growth and preventing the formation of biofilms. Furthermore, the biofilm matrix is composed of polysaccharides, and recent studies have shown that sugar molecules are also utilized as chemical messengers between bacterial species within the biofilm structure [10].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Properties of Latvian Honey against Causative Agents of Wound Infections

et al. 2023

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Honey is widely used in traditional medicine and modern wound healing biomaterial research as a broad-spectrum antimicrobial, anti-inflammatory and antioxidant agent. The study’s objectives were to evaluate the antibacterial activity and polyphenolic profiles of 40 monofloral honey samples collected from beekeepers in the territory of Latvia. The antimicrobial and antifungal activity of Latvian honey samples were compared with commercial Manuka honey and the honey analogue sugar solutions–carbohydrate mixture and tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, clinical isolates Extended-Spectrum Beta-Lactamases produced Escherichia coli, Methicillin-resistant Staphylococcus aureus and Candida albicans. Antimicrobial activity was evaluated with the well-diffusion method (80% honey solution w/v) and microdilution method. The honey samples with the highest antimicrobial potential were tested to prevent biofilm development and activity against a preformed biofilm. The principal component analysis of the antimicrobial properties of honey samples vs. polyphenolic profile was performed. Eleven honey samples exhibited antibacterial activity to all investigated bacteria. The antibacterial effect of the samples was most significant on the Gram-positive bacteria compared to the studied Gram-negative bacteria. Latvian honey presents promising potential for use in wound healing biomaterials, opening the possibility of achieving long-term antibacterial effects.

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“…In one investigation, Solidago extract demonstrated the capability to decrease E. coli adhesion to plastic surfaces by as much as 80%. In a separate study, Solidago extract was observed to disrupt the extracellular matrix within S. aureus biofilms, resulting in the detachment of bacteria from the biofilm structure [26].…”

Section: Discussionmentioning

confidence: 96%

Impact of Solidago virgaurea Extract on Biofilm Formation for ESBL-Pseudomonas aeruginosa: An In Vitro Model Study

Abdulkareem,

Alalwani,

Ahmed

et al. 2023

Pharmaceuticals

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The increasing disparity between antimicrobial resistance (AMR) and the development of new antimicrobials continues to pose a significant global health concern. However, plant extracts have shown promise in combating this issue either through their inherent antimicrobial activity or by serving as potential reservoirs of effective antimicrobial compounds. These compounds have the ability to target pathogenic biofilms and inhibit the production of extended-spectrum β -lactamases (ESBLs). However, there is limited research available on the antibacterial properties of goldenrod extract. Thus, the objective of this study was to investigate the impact of S. virgaurea (SV) extract on the viability and ability to form biofilms of ESBL-Pseudomonas aeruginosa (P. aeruginosa). A cross-sectional study was conducted from August 2022 to March 2023. The broth microdilution method was employed to determine the minimum inhibitory concentration (MIC) of the (SV) extract. Subsequently, the minimum bactericidal concentration (MBC) was determined based on the MIC values obtained. The antibiotic susceptibility of bacteria was evaluated using the Kirby disk diffusion assay and an Antimicrobial Susceptibility Testing (AST) card in conjunction with the Vitek-2 compact system. Biofilm formation was evaluated using Congo red and a 96-well Elisa plate, while the presence of extended-spectrum β-lactamases (ESBLs) was estimated by measuring the reduction of nitrocefin at a wavelength of 390 nm. In addition, treatment of biofilm and ESBL activity with SV extract using 96-well Elisa plate and nitrocefin hydrolyzing, respectively. The resistance rates of P. aeruginosa isolates to the tested antibiotics were as follows: Levofloxacin 33%, Ciprofloxacin 40%, Amikacin 49%, Meropenem 50%, Cefepime 70%, Ceftazidime 75%, Cefotaxime 85%, Piperacillin-Tazobactam 90%, Amoxiclav 97%, Ampicillin 99%, Ceftriaxone 100%. The prevalence of MDR-P. aeruginosa, XDR-P. aeruginosa, PDR-P. aeruginosa and non-MDR-PA were 40% (n = 40), 7% (n = 7), 3% (n = 3) and 50% (n = 50), respectively. From the GC–MS results, it was observed that the presence of Octadecane, Clioquinol, Glycerol tricaprylate, hexadecanoic acid, cis-13-octadecenoic acid, oleic acid and Propanamide were the major components in the Solidago extract. In the determination of plant crude extracts, the values ranged between 0.25 and 64 mg/mL against bacteria. The resulting activity of the extract showed a significant statistical relationship at a p-value ≤ 0.01 against ESBL production and biofilm formation in P. aeruginosa. The S. virgaurea extract exhibited effectiveness in inhibiting biofilm formation and combating P. aeruginosa strains that produce extended-spectrum β-lactamases (ESBLs).

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Antibacterial and Antibiofilm Effect of Unifloral Honeys against Bacteria Isolated from Chronic Wound Infections

Cited by 15 publications

References 64 publications

Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity

Chestnut Honey Is Effective against Mixed Biofilms at Different Stages of Maturity

Antimicrobial and Antibiofilm Properties of Latvian Honey against Causative Agents of Wound Infections

Impact of Solidago virgaurea Extract on Biofilm Formation for ESBL-Pseudomonas aeruginosa: An In Vitro Model Study

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