Antibacterial and antibiofilm activity of Ammi majus seed against Gram-positive bacteria

Adham, Aveen Nozad; Abdulah, Zheen Abdulelah

doi:10.15218/zjms.2017.018

Cited by 6 publications

(7 citation statements)

References 13 publications

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“…A rapid screening of the antibacterial and antifungal activity for AME and FEA extracts was done to confirm their previously reported activity [18,32,57]. Colorimetric broth micro-dilution method using XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide]-reduction assay was adopted to determine MIC against Gram positive bacteria: S. aureus (ATCC 25923) and S. mutans (ATCC 25175), as well as Gram negative bacteria: E. coli (ATCC 25922) and P. aeruginosa (ATCC9027).…”

Section: Antimicrobial Susceptibility Assay For Ame and Fea Extractsmentioning

confidence: 86%

Bio-guided fractionation of prenylated benzaldehyde derivatives as potent antimicrobial and antibiofilm from Ammi majus L. fruits-associated Aspergillus amstelodami

Salama

Fathallah

Raafat

et al. 2019

67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in Cooperat

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Ammi majus L.; Family Apiaceae; is a plant indigenous to Egypt. Its fruits contain bioactive compounds such as furanocoumarins and flavonoids of important biological activities. An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708) by morphology, microscopical characterization, and molecular identification. To our knowledge this is the first time an endophytic fungus has been isolated from the fruits. The antimicrobial activity of the Ammi majus ethanol fruits extract (AME) and fungal ethyl acetate extract (FEA) were investigated, where the FEA showed higher antimicrobial activity, against all the tested standard strains. Phytochemical investigation of the FEA extract yielded five prenylated benzaldehyde derivative compounds isolated for the first time from this species: Dihydroauroglaucin (1), tetrahydroauroglaucin (2), 2-(3,6-dihydroxyhepta-1,4-dien-1-yl)-3,6-dihydroxy-5-(dimethylallyl)benzaldehyde (3), isotetrahydroauroglaucin)4), and flavoglaucin (5). Structure elucidation was carried out using (1Hand 13C-NMR). Fractions and the major isolated compound 1 were evaluated for their antimicrobial and antibiofilm activity. Compound 1 showed high antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC) = 1.95 µg/mL, Streptococcus mutans (MIC = 1.95 µg/mL), and Staphylococcus aureus (MIC = 3.9 µg/mL). It exhibited high antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) = 7.81 µg/mL against Staphylococcus aureus and Escherichia coli biofilms and MBIC = 15.63 µg/mL against Streptococcus mutans and Candida albicans and moderate activity (MBIC = 31.25 µg/mL) against Pseudomonas aeruginosa biofilm. This reveals that dihydroauroglaucin, a prenylated benzaldehyde derivative, has a broad spectrum antimicrobial activity. In conclusion, it was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of Ammi majus may be due to the ability of its endophytic fungi to produce effective secondary metabolites.

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Section: Antimicrobial Susceptibility Assay For Ame and Fea Extractsmentioning

confidence: 86%

Bio-guided fractionation of prenylated benzaldehyde derivatives as potent antimicrobial and antibiofilm from Ammi majus L. fruits-associated Aspergillus amstelodami

Salama

Fathallah

Raafat

et al. 2019

67th International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research (GA) in Cooperat

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“…In 2017, Adham and Abdulah, reported the antibacterial and antibiofilm activities of A. majus fruits extract against only Gram-positive bacteria [18]. This gave us a clue to design a study using bio-guided fractionation method to specify the compounds responsible for antimicrobial and antibiofilm activity in addition to the investigation of the role of endophytic fungi to the previously reported activity of A. majus .…”

Section: Discussionmentioning

confidence: 99%

“…It was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of A. majus may be due to the endophytic metabolites. The ethanol extract of A. majus was reported to inhibit different species of Staphylococcus and Streptococcus bacteria with MIC ranging from 3.9–15.6 mg/mL and exhibited stronger antibiofilm activity against Staphylococcus species than Streptococcus species, however, none of the extracts used in that study was able to inhibit biofilm formation completely [18].…”

Section: Discussionmentioning

confidence: 99%

“…A rapid screening of the antibacterial and antifungal activity for AME and FEA extracts was done to confirm their previously reported activity [18,32,57]. Colorimetric broth micro-dilution method using XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2 H -tetrazolium-5-carboxanilide]-reduction assay was adopted to determine MIC against Gram positive bacteria: S. aureus (ATCC 25923) and S. mutans (ATCC 25175), as well as Gram negative bacteria: E. coli (ATCC 25922) and P. aeruginosa (ATCC9027).…”

Section: Methodsmentioning

confidence: 99%

“…Endophytes are considered as an area of research which can be a promising source for new drug discovery [17]. A. majus fruit extract was previously reported to possess antimicrobial activity [2,18]. Consequently, the current study aimed to isolate endophytic fungi associated with A. majus fruits and track the antimicrobial and antibiofilm activity using bio-guided fractionation method, which integrates analytical procedures with bioassays, representing a rapid and cost-effective method to discover potential useful fractions and pure compounds [19].…”

Section: Introductionmentioning

confidence: 99%

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Bio-Guided Fractionation of Prenylated Benzaldehyde Derivatives as Potent Antimicrobial and Antibiofilm from Ammi majus L. Fruits-Associated Aspergillus amstelodami

et al. 2019

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Ammi majus L.; Family Apiaceae; is a plant indigenous to Egypt. Its fruits contain bioactive compounds such as furanocoumarins and flavonoids of important biological activities. An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708) by morphology, microscopical characterization, and molecular identification. To our knowledge this is the first time an endophytic fungus has been isolated from the fruits. The antimicrobial activity of the Ammi majus ethanol fruits extract (AME) and fungal ethyl acetate extract (FEA) were investigated, where the FEA showed higher antimicrobial activity, against all the tested standard strains. Phytochemical investigation of the FEA extract yielded five prenylated benzaldehyde derivative compounds isolated for the first time from this species: Dihydroauroglaucin (1), tetrahydroauroglaucin (2), 2-(3,6-dihydroxyhepta-1,4-dien-1-yl)-3,6-dihydroxy-5-(dimethylallyl)benzaldehyde (3), isotetrahydroauroglaucin )4), and flavoglaucin (5). Structure elucidation was carried out using (1H- and 13C-NMR). Fractions and the major isolated compound 1 were evaluated for their antimicrobial and antibiofilm activity. Compound 1 showed high antimicrobial activity against Escherichia coli with minimum inhibitory concentration (MIC) = 1.95 µg/mL, Streptococcus mutans (MIC = 1.95 µg/mL), and Staphylococcus aureus (MIC = 3.9 µg/mL). It exhibited high antibiofilm activity with minimum biofilm inhibitory concentration (MBIC) = 7.81 µg/mL against Staphylococcus aureus and Escherichia coli biofilms and MBIC = 15.63 µg/mL against Streptococcus mutans and Candida albicans and moderate activity (MBIC = 31.25 µg/mL) against Pseudomonas aeruginosa biofilm. This reveals that dihydroauroglaucin, a prenylated benzaldehyde derivative, has a broad spectrum antimicrobial activity. In conclusion, it was observed that the MICs of the FEA are much lower than that of the AME against all susceptible strains, confirming that the antimicrobial activity of Ammi majus may be due to the ability of its endophytic fungi to produce effective secondary metabolites.

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Aatrilal (Ammi majus L.), an important drug of Unani system of medicine: A review

Usmani¹,

Jahan²,

Aleem³

et al. 2021

Journal of Ethnopharmacology

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Antibacterial and antibiofilm activity of Ammi majus seed against Gram-positive bacteria

Cited by 6 publications

References 13 publications

Bio-guided fractionation of prenylated benzaldehyde derivatives as potent antimicrobial and antibiofilm from Ammi majus L. fruits-associated Aspergillus amstelodami

Bio-guided fractionation of prenylated benzaldehyde derivatives as potent antimicrobial and antibiofilm from Ammi majus L. fruits-associated Aspergillus amstelodami

Bio-Guided Fractionation of Prenylated Benzaldehyde Derivatives as Potent Antimicrobial and Antibiofilm from Ammi majus L. Fruits-Associated Aspergillus amstelodami

Aatrilal (Ammi majus L.), an important drug of Unani system of medicine: A review

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