Antibacterial and anti-biofilm activities of probiotic Lactobacillus curvatus BSF206 and Pediococcus pentosaceus AC1-2 against Streptococcus mutans

Luan, Chang; Jiang, Ning; Zhou, Xiaoling; Zhang, Chuang; Zhao, Yanping; Li, Zhengqiang; Li, Chen

doi:10.1016/j.micpath.2022.105446

Cited by 18 publications

(16 citation statements)

References 57 publications

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“…It has been already reported that the above metabolites (malic acid, ascorbic acid, and citric acid) are said to be connected with dental caries 26,27 . Additionally, hydrogen peroxide, organic acids, bacteriocin, and other bioactive compounds are secreted into the environment as a result of antagonistic activity 7 …”

Section: Discussionmentioning

confidence: 99%

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Alleviation of dental caries by use of isolated potential probiotic and its characterization

Pallavi

Barik

Sahoo

et al. 2023

Biotech and App Biochem

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Streptococcus mutans plays a major role in biofilm formation and pathogenic bacterial adhesion. Here we investigated the abilities of our isolates from diverse conventional sources to characterize the beneficial bacteria for inhibition of S. mutans. Enterobacter cloacae PS‐74, a beneficial bacteria isolated from yoghurt, is gram negative, rod shaped, and resistant to acid, bile salt, and amylase. PS‐74 cell‐free supernatants (CFS) demonstrated highest zone of inhibition of 29 ± 1.7 mm. Further, the minimum inhibitory concentration (MIC) value of CFS PS‐74 was recorded to be 10 μL and its minimum bactericidal concentration (MBC) value was found to be 15μL which led to 99.9% log reduction of S. mutans. Moreover, the biofilm formation was reduced by 84.91% at MIC15 of CFS PS‐74 which alleviate the dental caries formation by S. mutans. This is the first report on E. cloacae PS‐74, which was studied for its probiotic properties to inhibit S. mutans MTCC‐890 due to the production of organic acids and employed in oral treatment.

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Section: Discussionmentioning

confidence: 99%

“…Various studies have discovered that probiotics or cell‐free supernatants (CFS) from different commercial strains can prevent tooth infections 6,7,8 . The effectiveness of lactic acid bacteria (LAB) has been reported for eliminating the dental pathogen 9,12 …”

Section: Introductionmentioning

confidence: 99%

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Pallavi

Barik

Sahoo

et al. 2023

Biotech and App Biochem

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“…Both probiotic strains exhibited auto-aggregation and coaggregation potential, these properties facilitate strains to adhere and colonize gingival epithelial cells and impede carcinogenic S. mutans from oral tissues. Moreover, the study reported that L. curvatus BSF206 and P. pentosaceus AC1-2 efficiently prevented S. mutans biofilm by downregulating the 10.3389/fnut.2022.959941 expression of key genes ftf, brpA, gtfA, and gtfB associated with biofilm formation (116).…”

Section: Auto-aggregation Biofilm Formation and Co-aggregationmentioning

confidence: 98%

“…Results concluded both these pathogenic bacteria were susceptible to the L. plantarumproduced antimicrobial compounds and co-aggregation (115). Luan et al evaluated the antimicrobial potential of Lactobacillus curvatus BSF206 and Pediococcus pentosaceus AC1-2 against the oral pathogen Streptococcus mutans (116). Both probiotic strains exhibited auto-aggregation and coaggregation potential, these properties facilitate strains to adhere and colonize gingival epithelial cells and impede carcinogenic S. mutans from oral tissues.…”

Section: Auto-aggregation Biofilm Formation and Co-aggregationmentioning

confidence: 99%

Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics

et al. 2022

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This review article addresses the strategic formulation of human probiotics and allows the reader to walk along the journey that metamorphoses commensal microbiota into target-based probiotics. It recapitulates what are probiotics, their history, and the main mechanisms through which probiotics exert beneficial effects on the host. It articulates how a given probiotic preparation could not be all-encompassing and how each probiotic strain has its unique repertoire of functional genes. It answers what criteria should be met to formulate probiotics intended for human use, and why certain probiotics meet ill-fate in pre-clinical and clinical trials? It communicates the reasons that taint the reputation of probiotics and cause discord between the industry, medical and scientific communities. It revisits the notion of host-adapted strains carrying niche-specific genetic modifications. Lastly, this paper emphasizes the strategic development of target-based probiotics using host-adapted microbial isolates with known molecular effectors that would serve as better candidates for bioprophylactic and biotherapeutic interventions in disease-susceptible individuals.

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“…RAW 264.7 cells were transferred to centrifuge tubes; then, total RNA was extracted according to instructions provided with the TaKaRa MiniBEST Universal RNA Extraction Kit (TaKaRa, Kyoto, Japan). Next, the Primescript™ RT reagent Kit (TaKaRa) was used to reverse transcribe approximately 1.0 µg of total RNA into cDNA [32]. The cDNA served as template for semi-quantitative real-time PCR to determine relative transcriptional expression levels of genes encoding TNF-α, IL-6, IL-1β, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS).…”

Section: Rna Extraction and Quantitative Real-time Polymerase Chain R...mentioning

confidence: 99%

Leuconostoc mesenteroides LVBH107 Antibacterial Activity against Porphyromonas gingivalis and Anti-Inflammatory Activity against P. gingivalis Lipopolysaccharide-Stimulated RAW 264.7 Cells

et al. 2022

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Probiotics, active microorganisms benefiting human health, currently serve as nutritional supplements and clinical treatments. Periodontitis, a chronic infectious oral disease caused by Porphyromonas gingivalis (P. gingivalis), activates the host immune response to release numerous proinflammatory cytokines. Here, we aimed to clarify Leuconostoc mesenterica (L. mesenteroides) LVBH107 probiotic effects based on the inhibition of P. gingivalis activities while also evaluating the effectiveness of an in vitro P. gingivalis lipopolysaccharide-stimulated RAW 264.7 cell-based inflammation mode. L. mesenteroides LVBH107 survived at acid, bile salts, lysozyme, and hydrogen peroxide conditions, auto-aggregated and co-aggregated with P. gingivalis, exhibited strong hydrophobicity and electrostatic action, and strongly adhered to gingival epithelial and HT-29 cells (thus exhibiting oral tissue adherence and colonization abilities). Moreover, L. mesenteroides LVBH107 exhibited sensitivity to antibiotics erythromycin, doxycycline, minocycline, ampicillin, and others (thus indicating it lacked antibiotic resistance plasmids), effectively inhibited P. gingivalis biofilm formation and inflammation (in vitro inflammation model), reduced the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and IL-1β) and inflammatory mediators (NO and PGE2), and decreased the expression levels of inflammation related genes. Thus, L. mesenterica LVBH107 holds promise as a probiotic that can inhibit P. gingivalis biofilm formation and exert anti-inflammatory activity to maintain oral health.

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Antibacterial and anti-biofilm activities of probiotic Lactobacillus curvatus BSF206 and Pediococcus pentosaceus AC1-2 against Streptococcus mutans

Cited by 18 publications

References 57 publications

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Alleviation of dental caries by use of isolated potential probiotic and its characterization

Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics

Leuconostoc mesenteroides LVBH107 Antibacterial Activity against Porphyromonas gingivalis and Anti-Inflammatory Activity against P. gingivalis Lipopolysaccharide-Stimulated RAW 264.7 Cells

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