Antibacterial activity of the streptomycetes isolated from marine soil sample

Nandhini, S.; Selvam, M. Masilamani

doi:10.1109/gtec.2011.6167694

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…The dried crude extract was dissolved in methanol, and stock concentration was prepared as 100 mg/mL. The crude extracts (3 to 7 mg) were used for the bioactivity against different pathogenic bacteria using well diffusion method with Mueller Hinton agar ( Nandhini and Selvam, 2011 ). Methanol used as a control and the bioactivity of extracts was noted based on the zone of inhibition.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

et al. 2017

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Bacterial secondary metabolites possess a wide range of biologically active compounds including antibacterial and antioxidants. In this study, a Gram-positive novel marine Actinobacteria was isolated from sea sediment which showed 84% 16S rRNA gene sequence (KT588655) similarity with Streptomyces variabilis (EU841661) and designated as Streptomyces variabilis RD-5. The genus Streptomyces is considered as a promising source of bioactive secondary metabolites. The isolated novel bacterial strain was characterized by antibacterial characteristics and antioxidant activities. The BIOLOG based analysis suggested that S. variabilis RD-5 utilized a wide range of substrates compared to the reference strain. The result is further supported by statistical analysis such as AWCD (average well color development), heat-map and PCA (principal component analysis). The whole cell fatty acid profiling showed the dominance of iso/anteiso branched C15–C17 long chain fatty acids. The identified strain S. variabilis RD-5 exhibited a broad spectrum of antibacterial activities for the Gram-negative bacteria (Escherichia coli NCIM 2065, Shigella boydii NCIM, Klebsiella pneumoniae, Enterobacter cloacae, Pseudomonas sp. NCIM 2200 and Salmonella enteritidis NCIM), and Gram-positive bacteria (Bacillus subtilis NCIM 2920 and Staphylococcus aureus MTCC 96). Extract of S. variabilis strain RD-5 showed 82.86 and 89% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and metal chelating activity, respectively, at 5.0 mg/mL. While H2O2 scavenging activity was 74.5% at 0.05 mg/mL concentration. Furthermore, polyketide synthases (PKSs types I and II), an enzyme complex that produces polyketides, the encoding gene(s) detected in the strain RD-5 which may probably involve for the synthesis of antibacterial compound(s). In conclusion, a novel bacterial strain of Actinobacteria, isolated from the unexplored sea sediment of Alang, Gulf of Khambhat (Gujarat), India showed promising antibacterial activities. However, fractionation and further characterization of active compounds from S. variabilis RD-5 are needed for their optimum utilization toward antibacterial purposes.

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Section: Methodsmentioning

confidence: 99%

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

et al. 2017

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“…Streptomyces cacaoi subsp. cacaoiAB184115 was isolated for the first time in 1932 by Waksman from cacao beans, and then Streptomyces cacaoi strain SU2 JF730119 from marine sediment (Nandhini and Selvam, 2011) and Streptomyces cacaoi subsp. cacaoi strain GU144523 from scalp (Pellegrini, 2012).…”

Section: Minimum Inhibitory Concentration (Mic) Of Bioactive Fractionmentioning

confidence: 99%

Phylogenetic and Antimicrobial Characteristics of a Novel Streptomyces sp. Ru87 Isolated from Egyptian Soil

Amin¹,

Tolba²,

Abolmaaty³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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“…Natural products are immense source for important novel compounds having antagonist activity against pathogenic microorganisms. The need to discover and develop more effective antibiotics with unique modes of action is greater than ever 8 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2016

IJPSR

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Many microorganisms thrive on the abundant nutrients in the rhizosphere and they produce antagonistic metabolites. The present study is an attempt to investigate the potent bacteria producing antibiotics against pathogenic microorganisms from the rhizosphere soil samples of Kanjamalai hills, Salem district, Tamilnadu, India. A total of 6 isolates of bacteria was obtained and screened for inhibitory activity against selected opportunistic pathogens. Potent bacterial isolate (A6) was identified as Pseudomonas aeruginosa by cultural, morphological and biochemical characterization. The antimicrobial activity of broth culture of Pseudomanas aeruginosa was also observed. The antimicrobial active compound was partially purified from the broth culture and its concentration was estimated to be 373.086 µg/ml. The obtained protein was separated in SDS-PAGE and its molecular weight was determined as 66.4 kD, 44.3 kD, 29.0 kD, 20.1 kD and 14.3 kD using standard molecular markers. The results showed that the isolated strain has high potential against Bacillus megaterium and Aspergillus niger. It can be further investigated to identify the antibacterial compounds produced and their mechanisms of inhibition.

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Antibacterial activity of the streptomycetes isolated from marine soil sample

Cited by 3 publications

References 3 publications

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

Antibacterial and Antioxidant Activities of Novel Actinobacteria Strain Isolated from Gulf of Khambhat, Gujarat

Phylogenetic and Antimicrobial Characteristics of a Novel Streptomyces sp. Ru87 Isolated from Egyptian Soil

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