Antibacterial activity of the lipopetides produced by Bacillus amyloliquefaciens M1 against multidrug-resistant Vibrio spp. isolated from diseased marine animals

Abstract

In this work, the antibacterial activity of the lipopeptides produced by Bacillus amyloliquefaciens M1 was examined against multidrug-resistant Vibrio spp. and Shewanella aquimarina isolated from diseased marine animals. A new and cheap medium which contained 1.0 % soybean powder, 1.5 % wheat flour, pH 7.0 was developed. A crude surfactant concentration of 0.28 mg/ml was obtained after 18 h of 10-l fermentation and diameter of the clear zone on the plate seeded with Vibrio anguillarum was 34 mm. A preliminary characterization suggested that the lipopeptide N3 produced by B. amyloliquefaciens M1 was the main product and contained the surfactin isoforms with amino acids (GLLVDLL) and hydroxy fatty acids (of 12-15 carbons in length). The evaluation of the antibacterial activity of the lipopeptide N3 was carried out against S. aquimarina and nine species of Vibrio spp.. It was found that all the Vibrio spp. and S. aquimarina showed resistance to several different antibiotics, suggesting that they were the multidrug resistance. It was also indicated that all the Vibrio spp. strains and S. aquimarina were sensitive to the surfactin N3, in particular V. anguillarum. The results demonstrated that the lipopeptides produced by B. amyloliquefaciens M1 had a broad spectrum of action, including antibacterial activity against the pathogenic Vibrio spp. with multidrug-resistant profiles. After the treatment with the lipopeptide N3, the cell membrane of V. anguillarum was damaged, and the whole cells of the bacterium were disrupted.

The Peptide Toxin Amylosin of Bacillus amyloliquefaciens from Moisture-Damaged Buildings Is Immunotoxic, Induces Potassium Efflux from Mammalian Cells, and Has Antimicrobial Activity

“…In addition to amylosin, B. amyloliquefaciens 19b produces surfactin and antifungal fengycin (2,43). Strains of B. amyloliquefaciens producing several antibacterial and antifungal linear and cyclic lipopeptides (e.g., fengycins, iturins, and surfactins) have also been isolated from, e.g., marine environments, mangrove forests, and plant rhizospheres (30,44,45). These reports together with our results suggest that the simultaneous production of several antimicrobial substances by B. amyloliquefaciens strains is widespread in nature and offers a competitive advantage to the producer microbe.…”

Isolation and characterisation of Bacillus spp. antagonistic to Vibrio parahaemolyticus for use as probiotics in aquaculture

“…So the culture and/or isolated cells with direct antimicrobial activity should be used as the source for novel antimicrobial substance extraction (Lai and Wong 2013). Other research confirmed that members of genus Bacillus can produce more than one type of antimicrobial metabolite (Aunpad and Panbangred 2012; Bacon et al 2012;Li et al 2012;Xu et al 2014). In addition, the strain of B. pumilus B16 or B. mojavensis J7 may produce different types, or amounts, of antimicrobial agents according to the variation in the potency of antagonism to different indicator bacteria (Table 4).…”

“…The results indicated that two the potential probiotic strains of B16 and J7 were closely related to Bacillus spp. which is well known for having the widely antimicrobial activity (Aunpad and Na-Bangchang 2007; Aunpad and Panbangred 2012; Li et al 2012;Xu et al 2014). The identities of 16S rRNA between strains of B16 and Bacillus pumilus ATCC 7061 and strains of J7 and Bacillus mojavensis SWFU16 were 100 and 99 % respectively (Fig.…”

A novel antioxidant and antimicrobial compound produced by Bacillus firmicutes

“…On the other hand, the methanol extract did not exhibit antimicrobial activity against E. coli. Several lipopeptide biosurfactants obtained from Bacillus amyloliquefaciens M1 [41], Bacillus mojavensis A21 [42] and Bacillus methylotrophicus [43] showed strong antibacterial activity. …”