Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

Widyaningrum, Naniek; Badie'ah, Badie'ah; Lestari, Sri

doi:10.24246/josse.v1i2p1-5

Cited by 2 publications

(3 citation statements)

References 3 publications

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“…Research by Widyaningrum et al (2017) showed that EGCG levels in green tea leaf pulp are 21.325% w/w while EGCG levels in green tea leaf are 60.98% w/w (Widyaningrum et al, 2015a). This indicates that the concentration of EGCG in green tea leaf is greater than that of in its waste.…”

Section: Discussionmentioning

confidence: 99%

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Development of Anti Acne Cream (w/o/w Multiple Emulsion) Containing Green Tea Leaf Waste

Widyaningrum

2018

Sains Med. J. Kedokt. dan Kesehat.

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Background: To date, green tea leaf waste is not well utilized, the waste is remain considered as a waste. Previous studies found that green tea leaf waste still have a fairly high EGCG content. EGCG has benefits as an antibacterial. Utilization of green tea leaf waste started from formulation until the diversification of multiple emulsion w/o/w products has not been reported.Objective: To develop anti acne cream (W/O/W) multiple emulsion containing green tea leaf waste and evaluate its antibacterial activity against acne-inducing bacteria of Propionibacterium acnes (P.acnes).Methods: Phase one of this research was green tea leaves was extracted by infundation and fractionation method using ethyl acetate and prepared in different concentration (1% to 6%). The second stage was ethyl acetate fraction of green tea leaf extract was tested for its antibacterial activity against P.acnes. Third stage was tested physical properties of formulation with w/o/w multiple emulsion.Results: The optimal antibacterial activity of ethyl acetate fraction of green tea leaf extract against P.acne was at the concentration of 6%, with inhibition zone of 32.6 mm ±0.57. The formula of w/o/w multiple emulsion loaded with green tea leaves waste of 6% active substance demonstrated a good physical properties which can spread 90.4 cm2 ± 0.03, pH 5.00 ± 0.02 and microscopic analysis showed multiple emulsion w/o/w.Conclusion: The concentration of 6% green tea leaf waste formulated in multiple emulsion had a good physical and antibacterial activity for a referred standard. It is necessary to test the stability of multiple emulsion formula.

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Section: Discussionmentioning

confidence: 99%

“…Green tea leaf waste has not been utilized. Based on preliminary studies, green tea leaf waste still contain EGCG although not as much as the first processed green leaf tea (Widyaningrum, 2017). Waste based product are expected to reduce the cost of production to benefit the middle society without reducing its quality and effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Development of Anti Acne Cream (w/o/w Multiple Emulsion) Containing Green Tea Leaf Waste

Widyaningrum

2018

Sains Med. J. Kedokt. dan Kesehat.

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“…Staphylococcus aureus (Trisina et al, 2021). (Savira & Suharsono, 2013;Widyaningrum et al, 2017;Herwin et al, 2018). Previous research has established a cream formulation from 96 % ethanol extract of green tea pulp in a multiple emulsion formula that has good physical qualities and antibacterial activity and may be utilized as a reference for formulation (Widyaningrum, 2017;Karmilah, 2021).…”

Section: Propionibacterium Acnes Andmentioning

confidence: 99%

FORMULASI MASKER GEL PEEL-OFF EKSTRAK ETANOL 96% AMPAS TEH HIJAU (Camellia sinensis Linn.) [FORMULATION OF A PEEL-OFF GEL MASK USING A 96% ETHANOL EXTRACT FROM GREEN TEA DREGS (Camellia sinensis Linn.)]

Trisina

Riskianto

Santoso

et al. 2022

JSTFaST

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Green tea (Camellia sinensis Linn.) is widely consumed in Indonesia, but the dregs which are waste have not been utilized optimally. A previous study revealed that a 96% ethanol extract from green tea dregs produces antibacterial activity against Staphylococcus aureus and Escherichia coli. Therefore, the purpose of this study was to increase the utilization of green tea dregs in cosmetic products in the form of a peel-off gel mask with an active ingredient of 96% ethanol extract from green tea dregs. The green tea dregs extract was made by the maceration method with 96% ethanol as the solvent. The peel-off gel mask was made in three formulas with different variations in the gel base, namely PVA 10% and HPMC 2%; PVA 8% and HPMC 3%; PVA 5% and HPMC 4%. The peel-off gel mask was produced, then it was evaluated by an organoleptic test, pH test, homogeneity test, spreadability test, and dry time test. The peel-off gel was stored for 10 days at two different temperatures, namely room temperature, and refrigerator temperature. The evaluation result was obtained that all three formulations of the peel-off gel mask met the criteria for good gel. However, after 10 days of storage at both storage temperatures, there was a change in the color of the three formulas. The Formula 3 (PVA 5% and HPMC 4%) stored at refrigerator temperature also showed an increment in dry time result. These results indicate the instability of the peel-off gel mask during storage. The peel-off gel mask formula from green tea dregs meets the requirements of gel preparations. Bahasa Indonesia Abstract:Teh hijau (Camellia sinensis Linn.) banyak dikonsumsi di Indonesia, namun ampas yang merupakan limbahnya belum dimanfaatkan secara optimal. Pada penelitian sebelumnya ekstrak etanol 96% ampas teh hijau memiliki aktivitas antibakteri pada Staphylococcus aureus and Escherichia coli. Oleh karena itu, tujuan dari penelitian ini adalah untuk meningkatkan pemanfaatan ampas teh hijau menjadi produk kosmetika berupa masker gel peel-off dengan bahan aktif ekstrak etanol 96% ampas teh hijau. Ekstrak ampas teh hijau dibuat dengan metode maserasi dengan pelarut etanol 96%. Masker gel peel-off dibuat dalam tiga formula dengan variasi basis gel yang berbeda, yaitu PVA 10% dan HPMC 2%; PVA 8% dan HPMC 3%; PVA 5% dan HPMC 4%. Masker gel peel off yang dihasilkan kemudian dievaluasi dengan uji organoleptik, uji pH, uji homogenitas, uji daya sebar, dan uji waktu kering. Gel peel off disimpan selama 10 hari pada dua suhu yang berbeda yaitu suhu ruang dan suhu lemari es. Hasil evaluasi diperoleh bahwa ketiga formulasi masker gel peel-off memenuhi kriteria sediaan gel yang baik. Namun setelah 10 hari penyimpanan pada kedua suhu penyimpanan, terjadi perubahan warna pada ketiga formula. Formula 3 (PVA 5% dan HPMC 4%) yang disimpan pada suhu lemari es juga menunjukkan peningkatan hasil waktu kering. Hasil ini menunjukkan ketidakstabilan sediaan selama penyimpanan.<div id="gtx-trans" style="position: absolute; left: -6px; top: 295.571px;"> </div>

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Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

Cited by 2 publications

References 3 publications

Development of Anti Acne Cream (w/o/w Multiple Emulsion) Containing Green Tea Leaf Waste

Development of Anti Acne Cream (w/o/w Multiple Emulsion) Containing Green Tea Leaf Waste

FORMULASI MASKER GEL PEEL-OFF EKSTRAK ETANOL 96% AMPAS TEH HIJAU (Camellia sinensis Linn.) [FORMULATION OF A PEEL-OFF GEL MASK USING A 96% ETHANOL EXTRACT FROM GREEN TEA DREGS (Camellia sinensis Linn.)]

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