Antibacterial Activity of Sonoran Propolis and Some of Its Constituents Against Clinically SignificantVibrioSpecies

Abstract

The aim of the present study was to evaluate the anti-Vibrio activity of propolis collected from three different areas of the Sonoran Desert in northwestern, Mexico [Pueblo de Alamos (PAP), Ures (UP), and Caborca (CP)]. The anti-Vibrio spp. activity of Sonoran propolis was determined by the broth microdilution method. UP propolis showed the highest antibacterial activity [minimal inhibitory concentration (MIC(50))<50 μg mL(-1)] against Vibrio spp. (UP>CP>PAP). UP propolis significantly inhibited the growth of Vibrio cholerae O1 serotype Inaba (MIC(50)<50 μg mL(-1)), V. cholerae non-O1 (MIC(50)<50 μg mL(-1)), V. vulnificus (MIC(50)<50 μg mL(-1)), and V. cholerae O1 serotype Ogawa (MIC(50) 100 μg mL(-1)), in a concentration-dependent manner. The UP propolis constituents, galangin and caffeic acid phenethyl ester (CAPE), exhibited a potent growth inhibitory activity (MIC(50) 0.05-0.1 mmol l(-1)) against V. cholerae strains (non-O1 and serotype Ogawa). The strong anti-Vibrio activity of Sonoran propolis and some of its chemical constituents (galangin and CAPE) support further studies on the clinical applications of this natural bee product against different Vibrio spp., mainly V. cholerae.

Phytochemical Constituents, Antioxidant, Cytotoxic, and Antimicrobial Activities of the Ethanolic Extract of Mexican Brown Propolis

“…The EtOH-soluble extract of sample GUA-4 was fractionated by chromatography on a VLC column and by Sephadex LH-20, giving 12 known compounds (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12). The compounds were identified as pinocembrin (1, 405.4 mg) [33], chrysin (2, 126.3 mg) [34], galangin (3, 56.7 mg) [33], alpinetin (4, 15.6 mg) [35], 5-methyl-pinobanksin ether (5, 16.8 mg) [36], dillenetin (6, 12.3 mg) [37], isorhamnetin (7, 24.6 mg) [38], 5-methylgalangin ether (8, 9.8 mg) [39], 5-methylchrysin ether (9, 16.3 mg) [40], ferulic acid (10, 24.9 mg) [33], syringic acid (11, 6.7 mg), and caffeic acid (12, 8.9 mg) [33] ( Figure S1) by means of 1D (Table S1) and 2D NMR spectral analysis.…”

“…To the best of our knowledge, this is the first report of dillenetin (6) as a propolis constituent. 5-methylpinobanksin (5), alpinetin (4), isorhamnetin (7), 5-methylgalangin ether (8), and 5-methylchrysin ether (9) were previously identified by diode array detection and ESI mass spectrometry (LC-DAD-ESI-MS) as constituents of Italian, Portuguese, and Czech propolis [41,42].…”

Advances in Pharmacological Activities and Chemical Composition of Propolis Produced in Americas

“…UP presented the highest antibacterial effect against Vibrio cholerae O1 serotype Inaba, V. cholerae non-O1, V. vulnificus (MIC 50 : <50 µg/mL), and V. coholerae O1 serotype Ogawa (MIC 50 : 100 µg/mL). The constituents CAPE and galangin presented a potent growth inhibitory activity (MIC 50 : 0.05-0.1 mM) against V. cholerae strains (non-O1 and serotype Ogawa) [93]. Additionally, the in vitro antiparasitic activity of Sonoran propolis against Giardia lamblia has been tested.…”

Modulatory effects of propolis samples from Latin America (Brazil, Cuba and Mexico) on cytokine production by human monocytes

“…Pinocembrin, pinobanksin 3-acetate, chrysin, caffeic acid phenethyl ester (CAPE), acacetin and galangin were identified as major constituents. [22] Mexican propolis has exhibited several biological properties, such as antiproliferative activity against cancer cell lines, [22,23] antibacterial effects [24,25] and free-radical scavenging activity. [23,24] Propolis immunomodulatory action has been investigated using murine macrophages and more recently human monocytes.…”