Antibacterial Activity of Some Medicinal Plants of Kashmir, J&K, India

Abstract

The study was conducted to find out the activity of some medicinal plants against bacterial isolates. The bacterial isolates from milk samples of infected quarters of cows were investigated for in-vitro drug sensitivity by standard disc diffusion technique (Bauer et al., 1966). Cultural examination of milk samples was done by method described by Quin et al. (2004) in which predominant isolates were Staph. (55.55%), E. coli (22.22%), Strepto. agalactiae (16.66%) and other isolates as 5.5% and were subjected to in-vitro antibacterial sensitivity test to selected herbal extracts and standard antibiotic (cefuroxime) (Table-1). The four different aqueous concentrations of the herbs namely Fumaria indica, Adiantum capillus, Nepata cataria, Levandula stoeches and Borago officinalis collected from registered herbal shops prepared by standard procedure as 25mg/ml, 50mg/ml, 75mg/ml and 100mg/ml were used. The results indicated that aqueous extract of Fumaria indica, Adiantum capillus and Napeta cataria against Staphylococcus aureus, E.coli, Streptococcus agalactia and K. pneumonia exhibited maximum zone of inhibition 20.0±1.21, 21.0±0.19, 13.0±0.37 17.0±0.21; 18±0.41, 12±0.21, 13±0.31, 15±0.31 and 15±0.33, 16.01±0.19 14.09±0.37, 13.31±0.41 at 100 mg/ml respectively which was significantly low as compared to standard drug (cefuroxime) at 30 microgram concentration. Aqueous extract of Levandula stoeches against Staphylococcus aureus and E. coli exhibited maximum zone of inhibition 18.0±0.33 and 13.0±0.141 at 100 mg/ml respectively. Borago officinalis has shown nil to non-significant bacterial growth inhibition activity.

Antibacterial potential of Antimicrobial peptides containing whole proteins of two Adiantum species from Himalaya against selected human bacterial pathogens

“…Adiantum has been reported for its various medicinal properties, specially antibacterial and antiviral, antifungal activities. Antibacterial activity of organic solvent extracts of different Adiantum species against P. aeruginosa (Thomas, 2017;Zhang et al, 2019), E. coli (Johnson et al, 2017) , K. pneumoniae (Johnson et al, 2017;Reshi et al, 2017), and S. aureus (Reshi et al, 2017;Thomas, 2017), Streptococcus agalactia (Reshi et al, 2017), Serratia marcescens (Thomas, 2017), Micrococcus lysodeikticus (Zhang et al, 2019), Bacterium paratyphosum B (Zhang et al, 2019), Bacillus subtilis have also been reported by various research groups.…”

Borago Officinalis L.: A Review Oon Extraction, Phytochemical, and Pharmacological Activities

“…[99,100] Only two studies employed decoction for Borago officinalis leaves. [35,53] Zemmouri et al (2019) [35] reported 35.48 � 2.70 mg GAE/g of extract for phenolic compounds, 20.79 � 2.95 mg QE/g of extract for flavonoids, 10.88 � 0.18 mg R/g of extract for flavanols, 3.14 � 0.60 mg C/g of extract for tannins, and 0.09 � 0.01 mg CÀ 3-G/g of extract for anthocyanins in the B. officinalis leaves extract obtained with water/plant matérial of 50 : 1 (mL/g) for 20 minutes with occasional agitation. Levels of bioactive compounds, particularly phenolic compounds, flavonoids, and tannins, were lower compared to the ethanol extract obtained by maceration.…”

“…The antibacterial properties of a methanolic extract of B. officinalis leaves using the agar diffusion method evidenced low antibacterial activity against Escherichia coli and Pseudomonas aeruginosa and moderate activity against Staphylococcus aureus. [56] In Reshi et al ( 2017), [53] various concentrations of the aqueous extract of B. officinalis leaves were tested on S. aureus, E. coli, S. agalactiae, and K. pneumoniae using the agar disk diffusion method (DDM), and cefuroxime as a positive control. This extract showed non-significant activity against the growth of the tested bacteria.…”

“…Suitable for water‐soluble bioactive substances, stable at high temperatures, [99–101] the decoction consists of boiling the raw plant in a specified volume of water for a defined time, followed by cooling and filtration [99,100] . Only two studies employed decoction for Borago officinalis leaves [35,53] . Zemmouri et al.…”

“…In Reshi et al. (2017), [53] various concentrations of the aqueous extract of B. officinalis leaves were tested on S. aureus , E. coli , S. agalactiae , and K. pneumoniae using the agar disk diffusion method (DDM), and cefuroxime as a positive control. This extract showed non‐significant activity against the growth of the tested bacteria.…”