Antibacterial activity of silver nanoparticles synthesized using endophytic fungus—<i>Penicillium cinnamopurpureum</i>

Dinesh, B.; Monisha, N.; Shalini, H; Prathap, G. K.; Poyya, Jagadeesha; Shantaram, Manjula; Hampapura, Jayanth S.; Karigar, Chandrakant S.; Joshi, Chandrashekhar G.

doi:10.1080/00387010.2021.2010764

Cited by 14 publications

(9 citation statements)

References 49 publications

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“…The peak at 3440 cm −1 indicates stretching mode of the NH group (Chandraker et al, 2019). Peaks observed at 2926 and 2856 cm −1 correspond to CH stretching of alkanes (Dinesh et al, 2021). The peak corresponds to 2345 cm −1 related to CH stretching of the methylene group (Saravanakumar et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

Green synthesis of AgNPs using Delonix regia bark for potential catalytic and antioxidant applications

Bala

Rani

2023

Microscopy Res & Technique

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Nanoparticle synthesis from plant resources has recently gained significant impact due to its low cost, simple equipment requirements, and ease of availability. In this work, DR‐AgNPs were synthesized using bark extract of Delonix regia (D. regia) plant under microwave irradiation. The formation of DR‐AgNPs has been confirmed with UV–Vis, XRD, FTIR, FESEM, HRTEM, EDS, DLS, and zeta potential analysis. Catalytic and antioxidant activities were tested on synthesized spherical nanoparticles with a size range of 10–48 nm. The effects of pH and catalyst dosage on the methylene blue (MB) dye degradation were carried out. It was observed from the treatment results that 95% MB dye degradation efficiency was achieved within 4 min with a degradation rate constant of 0.772 min−1. The synthesized nanoparticles showed a strong antioxidant property when analyzed by a 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging assay. The calculated IC50 value for DR‐AgNPs was 37.1 ± 0.12 μg mL−1. Therefore, DR‐AgNPs are excellent in both catalytic and antioxidant activities when compared to previously reported works. Highlights Green synthesis of silver nanoparticles (DR‐AgNPs) using Delonix regia bark extract. The catalytic activity of DR‐AgNPs is remarkable against Methylene Blue. DR‐AgNPs also have a strong DPPH radical antioxidant effect. Short degradation time, high degradation rate constant, and a good scavenging activity are key features of this study compared to previously reported works.

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Section: Resultsmentioning

confidence: 99%

Green synthesis of AgNPs using Delonix regia bark for potential catalytic and antioxidant applications

Bala

Rani

2023

Microscopy Res & Technique

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“…The presence of oxygen could be assigned to emission of X-rays from free amino groups from Penicillium solitum . Similarly, 1.35% carbon presence in EDX indicated the presence of sugars in the fungal mass extract ( Dinesh et al., 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…Bio reduction of Ag + ions using the cell-free culture supernatant of Penicillium solitum was monitored by FTIR (Thermo Scientific, USA) and the spectra were recorded at an interval between 4000 and 400 cm −1 ( Dinesh et al., 2021 ; Rose et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

First identification of potato tuber rot caused by Penicillium solitum, its silver nanoparticles synthesis, characterization and use against harmful pathogens

Shah,

Shan,

Baig

et al. 2023

Front. Plant Sci.

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Potato is one of the highly consumed vegetable crop grown in different regions across Pakistan that is affected by fungal diseases. The current research was conducted to identify fungal pathogen causing mold-like disease of potato in Khyber Pakhtunkhwa (KP), Pakistan. For molecular identification and characterization of the fungal disease; potato tuber samples were collected followed by culturing on potato dextrose agar (PDA). Based on morphological features, the pathogen was identified as a Penicillium species. This result was obtained in 45 different isolates from potato tubers. Molecular identification was done using β-tubulin primers and ITS5 sequencing of 13 different isolates that releveled 98% homology with BLAST (GenBank accession no. KX958076) as Penicillium solitum (GenBank accession nos. ON307317; ON307475 and ON310801). Phylogenetic tree was constructed that showed Penicillium solitum prevalence along with Penicillium polonicum and Penicillium citrinum on potato tubers. Based on this, Penicillium solitum based silver nanoparticles (Ag NPs) were synthesized and characterized using UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray (EDX) and field emission scanning electron microscopy (FE SEM). UV-analysis showed a characteristic peak at 410 nm confirming synthesis of Penicillium solitum based Ag NPs. This was further confirmed by XRD followed by EDX and SEM that showed face cubic crystal structure with Ag as major constituent of 18 nm formed spherical Ag NPs. FTIR showed band stretching of O-H, N-O and C-H of biological origin. Similarly, Penicillium solitum based Ag NPs presented strong anti-bacterial and anti-fungal activity at 0.5 level of significance LSD. According to our knowledge, this is the first report of Penicillium solitum identification in Pakistan, its Ag NPs synthesis and characterization to be used against pathogens of agricultural significance.

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“…The endophytes P. canescens and P. commune showed antimicrobial activities against to the human pathogens ( Gao et al, 2011 ; Malhadas et al, 2017 ) and the phytopathogenic fungi ( Bertinetti et al, 2009 ). Other species, such as P. cinnamopurpureum ( Dinesh et al, 2022 ), P. chrysogenum ( Orfali et al, 2022 ), and P. citrinum ( Kumari et al, 2021 ), had also been reported as being prolific producers of antimicrobial compounds. However, no studies have reported the antibacterial activity of P. macrosclerotiorum , which was identified as a new species of the genus Penicillium in 2017 ( Wang et al, 2007 ; Xu et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Cultivable endophytic fungal community associated with the karst endemic plant Nervilia fordii and their antimicrobial activity

et al. 2022

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Endophytic fungi from medicinal plants with specific pharmacological functions attract much attention to provide the possibility of discovering valuable natural drugs with novel structures and biological activities. Nervilia fordii is a rare and endangered karst endemic plant that is used as medicine and food homology in Guangxi, China. These plants have been reported to have antimicrobial, antitumor, antiviral, and anti-inflammatory activities. However, few studies have focused on the diversity and antibacterial activity of endophytic fungi from N. fordii. In the present study, 184 endophytic fungi were isolated from the healthy tissues of N. fordii, and their molecular diversity and antimicrobial activities were analyzed for the first time. These fungi were categorized into 85 different morphotypes based on the morphological characteristics and the similarity between the target sequence and the reference sequence in the GenBank database. With the exception of 18 unidentified fungi, the fungal isolates belonged to at least 2 phyla, 4 classes, 15 orders, 45 known genera, and 45 different species, which showed high abundance, rich diversity, and obvious tissue specificity. All isolates were employed to screen for their antimicrobial activities via the agar diffusion method against Escherichia coli, Staphylococcus aureus, and Candida tropicalis. Among these endophytes, eight strains (9.41%) displayed inhibitory activity against E. coli, 11 strains (12.94%) against S. aureus, and two strains (2.35%) against C. tropicalis, to some extent. In particular, our study showed for the first time that the fungal agar plugs of Penicillium macrosclerotiorum 1151# exhibited promising antibacterial activity against E. coli and S. aureus. Moreover, the ethyl acetate (EA) extract of P. macrosclerotiorum 1151# had antibacterial effects against E. coli and S. aureus with a minimum inhibitory concentration (MIC) of 0.5 mg ml–1. Further research also confirmed that one of the antimicrobial compounds of P. macrosclerotiorum 1151# was methyl chloroacetate and exhibited excellent antibacterial activity against E. coli and S. aureus up to 1.71-fold and 1.13-fold compared with tetracycline (TET) (5 mg ml–1), respectively. Taken together, the present data suggest that various endophytic fungi of N. fordii could be exploited as sources of novel natural antimicrobial agents.

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Antibacterial activity of silver nanoparticles synthesized using endophytic fungus—Penicillium cinnamopurpureum

Cited by 14 publications

References 49 publications

Green synthesis of AgNPs using Delonix regia bark for potential catalytic and antioxidant applications

Green synthesis of AgNPs using Delonix regia bark for potential catalytic and antioxidant applications

First identification of potato tuber rot caused by Penicillium solitum, its silver nanoparticles synthesis, characterization and use against harmful pathogens

Cultivable endophytic fungal community associated with the karst endemic plant Nervilia fordii and their antimicrobial activity

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