ANTIBACTERIAL ACTIVITY OF SILVER MICROPARTICLES ENCAPSULATED WITH HONEYS FROM Apis mellifera AND Scaptotrigona bipunctata

Clébis, Victor Hugo; Proni, Edson Aparecido; Puño-Sarmiento, Juan; Kobayashi, Renata Katsuko Takayama; Nakazato, Gerson

doi:10.22533/at.ed.89421100823

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“…The 96-well plates were incubated for 24 h at 37 °C and; after incubation, the MIC values were defined as the lowest concentration of microcompound that inhibited visible growth. The results obtained were compared to the microcompounds MIC obtained in a previous study (see the Results section) [46] .…”

Section: Microdilution Assays Of Washed Microcompoundsmentioning

confidence: 99%

“…Table 6 displays Minimal Inhibitory Concentrations (MICs)-estimated by Ag concentration obtained through atomic absorption spectroscopy -of washed HAM and HSB microcompounds, against S. aureus ATCC 29231 and E. coli ATCC 8739, and compared against microcompound MICs (without centrifugation) described in a previous article [46] . MIC of all non-centrifuged microcompounds in a previous analysis [46] varied between 15 µM and 250 µM (or 1.62-27 μg•mL -1 ) and were similar to MIC findings described in the literature, and thus a promising result [67][68][69][70][71][72][73][74][75][76][77] . However, MIC against E. coli and S. aureus strains increased for most types of microcompounds centrifuged from the honey solution (Table 6).…”

Section: Mic Assays Of Before and After Centrifugationmentioning

confidence: 99%

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Comparison of the Antibacterial Effect of Different Biological Silver Nanoparticles Synthetized and Integrated with Honeys

Clébis¹,

Scandorieiro²,

Spinosa

et al. 2022

nefm

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This study compares the morphologies, zeta potentials, and antibacterial effects a total 12 different microcompounds containing honey and silver nanoparticles, in a novel study of the difference between honey samples in nanoparticle synthesis, as well as the antibacterial interaction that those honey samples can have with the silver nanoparticles synthetized using them. Microcompounds were synthetized by combining silver nitrate solution with a honey sample and performing one of methods of biogenic synthesis: sunlight exposure, basification to pH 5 or basification to pH 10. Samples of each microcompound were also submitted to heat treatment, obtaining thus heated variants. Morphology and size data were obtained by Dynamic Light Scattering (DLS) analysis and Scanning Electron Microscopy (SEM); while zeta potential was measured by Electrophoretic Light Scattering. Broth microdilution, time-kill curves and SEM were used to access the antibacterial effect. Mean diameter of particles inside all microcompounds varied between 100 nm and 150 nm; and the zeta potential varied depending on the honey used. Minimal Inhibitory Concentrations (MIC) of microcompounds were between 15 μM and 500 μM. Time-kill curves showed that microcompounds had a faster and stronger effect against Escherichia coli than Staphylococcus aureus. Microcompounds obtained by basification to pH 5 or by sunlight were bactericidal, as they were capable of inhibiting bacterial growth (resulting in an antibacterial efficiency of 100% in 24 hours) at 125 μM against S. aureus and 62.5 μM against E. coli. SEM micrographs showed bacterial cells with lower cell density, blebs and other alteration after microcompound treatment.

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Section: Microdilution Assays Of Washed Microcompoundsmentioning

confidence: 99%

Section: Mic Assays Of Before and After Centrifugationmentioning

confidence: 99%

Comparison of the Antibacterial Effect of Different Biological Silver Nanoparticles Synthetized and Integrated with Honeys

Clébis¹,

Scandorieiro²,

Spinosa

et al. 2022

nefm

View full text Add to dashboard Cite

show abstract

ANTIBACTERIAL ACTIVITY OF SILVER MICROPARTICLES ENCAPSULATED WITH HONEYS FROM Apis mellifera AND Scaptotrigona bipunctata

Abstract: Direitos para esta edição cedidos à Atena Editora pelos autores. Open access publication by Atena Editora Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição-Não-Comercial-NãoDerivativos 4.0 Internacional (CC BY-NC-ND 4.0).

Cited by 1 publication

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Comparison of the Antibacterial Effect of Different Biological Silver Nanoparticles Synthetized and Integrated with Honeys

Comparison of the Antibacterial Effect of Different Biological Silver Nanoparticles Synthetized and Integrated with Honeys

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