Antibacterial activity of selected snake venoms on pathogenic bacterial strains

Boda, Francisc; Mare, Anca Delia; Szabó, Zoltán‐István; Berța, Lavinia; Curticăpean, Augustin; Dogaru, Maria Titica; Man, Adrian

doi:10.2478/rrlm-2019-0015

Cited by 3 publications

(2 citation statements)

References 44 publications

(50 reference statements)

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“…Aside from their predictive value, it is crucial to consider the underlying antibacterial mechanisms of protein components individually as well as their functional interactions. Although specific proteins within a protein family may differ between venoms, they exhibit similar cytotoxic activities [28,[36][37][38]. For all Elapidae, our analyses suggest PLA 2 , svMP, and WAP/KUN as the best predictors for antibacterial effectiveness.…”

Section: Predictions Vs Mechanismsmentioning

confidence: 76%

Predicting antibacterial activity from snake venom proteomes

et al. 2020

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The continued evolution of antibiotic resistance has increased the urgency for new antibiotic development, leading to exploration of non-traditional sources. In particular, snake venom has garnered attention for its potent antibacterial properties. Numerous studies describing snake venom proteomic composition as well as antibiotic efficacy have created an opportunity to synthesize relationships between venom proteomes and their antibacterial properties. Using literature reported values from peer-reviewed studies, our study generated models to predict efficacy given venom protein family composition, snake taxonomic family, bacterial Gram stain, bacterial morphology, and bacterial respiration strategy. We then applied our predictive models to untested snake species with known venom proteomic compositions. Overall, our results provide potential protein families that serve as accurate predictors of efficacy as well as promising organisms in terms of antibacterial properties of venom. The results from this study suggest potential future research trajectories for antibacterial properties in snake venom by offering hypotheses for a variety of taxa.

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Section: Predictions Vs Mechanismsmentioning

confidence: 76%

Predicting antibacterial activity from snake venom proteomes

et al. 2020

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“…In the present study, antibacterial activity was not found for S. aureus and E. coli but was determined for Pseudomonas aeruginosa, obtaining a MIC of 50 and a MBC of 100 µg/mL for C. triseriatus and C. ravus (CT2 and CR3). According to Boda et al, 2019, the antibacterial activity of venoms from viperid species is probably due to their content of proteins with proteolytic activity [19].…”

Section: Antibacterial Activitymentioning

confidence: 99%

Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

Zaragoza-Bastida

Flores-Aguilar

Aguilar-Castro

et al. 2020

Animals

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Simple Summary: Rattlesnakes (Crotalus ravus and Crotalus triseriatus) have some compounds that resemble polypeptides and proteins in their venoms which can be used in therapeutic treatment as antibacterial compounds. The aim of the present study is to evaluate the antibacterial and hemolytic activity of two rattlesnake venoms. The results of the present study indicate that the evaluated venoms have bactericidal activity against Pseudomonas aeruginosa, an important bacterium that affects animals and humans, thereby providing a new and efficient treatment alternative against this pathogenic bacterium.Abstract: Rattlesnakes have venoms with a complex toxin mixture comprised of polypeptides and proteins. Previous studies have shown that some of these polypeptides are of high value for the development of new medical treatments. The aim of the present study is to evaluate, in vitro, the antibacterial and hemolytic activity of Crotalus triseriatus and Crotalus ravus venoms. A direct field search was conducted to obtain Crotalus triseriatus and Crotalus ravus venom samples. These were evaluated to determine their antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa through the techniques of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Hemolytic activity was also determined. Antibacterial activity was determined for treatments (Crotalus triseriatus 2) CT2 and (Crotalus ravus 3) CR3, obtaining a Minimum Inhibitory Concentration of 50 µg/mL and a Minimum Bactericidal Concentration of 100 µg/mL against Pseudomonas aeruginosa. CT1 (Crotalus triseriatus 1), CT2, and CR3 presented hemolytic activity; on the other hand, Crotalus ravus 4 (CR4) did not show hemolytic activity. The results of the present study indicate for the first time that Crotalus triseriatus and Crotalus ravus venoms contain some bioactive compounds with bactericidal activity against Pseudomonas aeruginosa which could be used as alternative treatment in diseases caused by this pathogenic bacterium.

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Antimicrobial activity of different substituted meso-porphyrin derivatives

Sălăgeanu¹,

Muntean

George

et al. 2020

Revista Romana De Medicina De Laborator

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The increasing resistance against classical antibiotic treatment forces the researchers to develop novel non-toxic antimicrobial agents. The aim of this study was to determine the antimicrobial properties of seven different porphyrins having distinctive hydrophobicity/hydrophilicity: P1 meso-tetra(4-methoxy-phenyl)porphyrin, P2 Zn(II)-meso-5,10,15,20-tetrapyridylporphyrin, P3 meso-tetra(p-tolyl)porphyrin, P4 5,10,15,20-tetraphenylporphyrin; P5 (5,10,15,20-tetraphenylporphinato) dichlorophosphorus(V) chloride, P6 5,10,15,20-tetrakis-(N-methyl-4-pyridyl) porphyrin-Zn(II) tetrachloride, P7 Zn(II)-5,10,15,20-meso-tetrakis-(4-aminophenyl)porphyrin. The meso-porphyrin derivatives were screened for their antimicrobial activity against six reference strains: Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC27853 and Candida albicans ATCC 10231. The antimicrobial activity of these samples was evaluated by the agar disk diffusion method and dilution method, with the determination of the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC) and the minimum fungicidal concentration (MFC). The most significant result is provided by the water-soluble P5 manifesting an obvious antimicrobial activity against Streptococcus pyogenes. On the other hand, P6 is a moderately active derivative against Streptococcus pyogenes and Escherichia coli and P7 presents moderate activity against Streptococcus pyogenes and Staphylococcus aureus. All the tested porphyrin bases, presenting hydrophobic character, have no antimicrobial activity under the investigated conditions. The common characteristics of the porphyrins that act as promising antimicrobial agents in the non-irradiated methods are: the cationic nature, the increased hydrophilicity and the presence of both amino functional groups grafted on the porphyrin ring and the coordination with Zn or phosphorus in the inner core.

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Antibacterial activity of selected snake venoms on pathogenic bacterial strains

Cited by 3 publications

References 44 publications

Predicting antibacterial activity from snake venom proteomes

Predicting antibacterial activity from snake venom proteomes

Antibacterial and Hemolytic Activity of Crotalus triseriatus and Crotalus ravus Venom

Antimicrobial activity of different substituted meso-porphyrin derivatives

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