Antibacterial activity of Orthosiphon stamineus towards Staphylococcus aureus and methicilin resistant Stahpylococcus aureus (MRSA)

Abstract

In this study, antibacterial activity of crude extract from Orthosiphon stamineus was analyzed. Extraction through immersing method was performed using methanol and aqueous extraction from whole plant (except root). Spot test and MIC was conducted for both extracts to screen for their antibacterial activity against Staphylococcus aureus and 10 methicillin resistant Staphylococcus aureus (MRSA). No antibacterial activity was observed for all strain tested in the spot test assay. However, observation through MIC test showed that both extracts have antibacterial activity. The aqueous extract showed antibacterial activity in the range of 6.25 mg/mL to 50 mg/mL against S. aureus, MRSA strains (ATCC 4330, TM, WM2, K31, K32, K39, and K52). The antibacterial activity of methanol extract was found to be in the range of 12.5 mg/mL to 50 mg/mL). Methanol extract was found to be more active against S. aureus and 9 MRSA strains (ATCC 4330, BM3, TM, WM2, K31, K32, K39, K52 and K59. The aqueous extract tested on S. aureus has the highest MIC value which is 6.25 mg/mL. In conclusion, O. stamineus was found to have potential as an anti-MRSA agent. Further analysis has to be done in order to develop a novel anti-MRSA drug to combat multiple resistant bacteria.