Antibacterial activity of nano zinc oxide green-synthesised from
            <i>Gardenia thailandica</i>
            triveng. Leaves against
            <i>Pseudomonas aeruginosa</i>
            clinical isolates: in vitro and in vivo study

Alotaibi, Badriyah; Negm, Walaa A.; Elekhnawy, Engy; El‐Masry, Thanaa A.; Elharty, Mohamed E; Saleh, Asmaa; Abdelkader, Dalia H.; Mokhtar, Fatma Alzahraa

doi:10.1080/21691401.2022.2056191

Cited by 41 publications

(21 citation statements)

References 36 publications

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“…We also determined the minimum inhibitory concentrations (MICs) of GINK and SOTE using the broth microdilution method, as previously described [ 24 ], in 96-well microtitration plates. The MIC values of GINK and SOTE (solubilized in DMSO concentration ≤ 1%) were identified for each bacterial isolate as the lowest extract concentration which exhibited complete inhibition of the bacterial growth (i.e., absence of turbidity).…”

Section: Methodsmentioning

confidence: 99%

“…Animals were randomly grouped into three groups, then all of them were administered bacterial suspension of E. faecalis (0.2 mL, 2.0 × 10 7 CFU/mL) for two days by subcutaneous (SC) injection to induce systemic infection. The three groups were as follows [ 24 , 32 ]:…”

Section: Methodsmentioning

confidence: 99%

“…Serum was utilized, in the current study, for assessment of liver function using commercial kits of ELISA. The levels of alanine aminotransferase (ALT) (ab234579, Abcam, Cambridge, UK), aspartate aminotransferase (AST) (ab263883, Abcam, Cambridge, UK), albumin (ab108789, Abcam, Cambridge, UK), total proteins (BioRad, Hercules, CA, USA), and bilirubin (MBS730053, MyBioSource, San Diego, CA, USA) were measured in all groups [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

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Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

et al. 2022

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Enterococcus species possess many virulence factors that have an essential role in exacerbating the infections caused by them. The current study aimed to evaluate the effect of the secondary metabolites ginkgetin (GINK) and sotetsuflavone (SOTE), isolated from Cycas media R. Br dichloromethane fraction, on Enterococcus faecalis (E. faecalis) isolates for the first time. The antibacterial and antivirulence activities of the isolated compounds were investigated using docking studies and in vitro by determination of the minimum inhibitory concentrations (MICs). Additionally, flow cytometry and scanning electron microscope (SEM) were utilized to assess the effect of SOTE on the tested bacteria. Moreover, crystal violet assay and qRT-PCR were used to test the effect of SOTE on the biofilm-forming ability of E. faecalis isolates. In addition, a systemic infection model was utilized in vivo to investigate the antibacterial activity of SOTE. We found that both GINK and SOTE showed a good affinity for the five proteins enrolled in the virulence of E. faecalis, with SOTE being the highest, suggesting the possible mechanisms for the antivirulence activity of both ligands. In addition, SOTE exhibited a higher antibacterial activity than GINK, as the values of the MICs of SOTE were lower than those of GINK. Thus, we performed the in vitro and in vivo assays on SOTE. However, they did not exhibit any significant variations (p > 0.05) in the membrane depolarization of E. faecalis isolates. Moreover, as evaluated by SEM, SOTE caused distortion and deformation in the treated cells. Regarding its impact on the biofilm formation, it inhibited the biofilm-forming ability of the tested isolates, as determined by crystal violet assay and qRT-PCR. The in vivo experiment revealed that SOTE resulted in a reduction of the inflammation of the liver and spleen with an increase in the survival rate. SOTE also improved the liver-function tests and decreased tumor necrosis factor-alpha using immunostaining and the inflammation markers, interleukins (IL-1β and IL-6), using ELISA. Thus, we can conclude that SOTE could be a promising compound that should be investigated in future preclinical and clinical studies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

et al. 2022

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“…Digital images of the wounds were recorded on days zero, three, and seven. The wound areas (cm 2 ) were measured using a ruler on these days to assess healing efficacy [ 25 ]. The wound healing percentage was calculated using the following equation:

n represents day three or day seven.…”

Section: Methodsmentioning

confidence: 99%

“…qRT-PCR was carried out as previously described using the glyceraldehyde-phosphate dehydrogenase (GAPDH) gene as a reference gene. The relative expression of fibronectin and PDGF genes was determined (primers listed in Table S3 ) [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Silver Nanoparticles Prepared Using Encephalartos laurentianus De Wild Leaf Extract Have Inhibitory Activity against Candida albicans Clinical Isolates

Alherz

Negm

Elekhnawy

et al. 2022

JoF

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Candida albicans is a major human opportunistic pathogen causing infections, which range from cutaneous to invasive systemic infections. Herein, the antifungal and anti-biofilm potential of silver nanoparticles (AgNPs) green synthesized in the presence of Encephalartos laurentianus leaf extract (ELLE) were investigated. The bioactive chemicals of ELLE, including phenolics, flavonoids, and glycosides were identified and quantified for the first time. AgNPs showed minimum inhibitory concentration (MIC) values against C. albicans clinical isolates ranging from 8 to 256 µg/mL. In addition, AgNPs significantly decreased biofilm formation. The impact of AgNPs on the expression of the genes encoding biofilm formation was assessed using qRT-PCR. AgNPs had a beneficial role in the macroscopic wound healing, and they resulted in complete epithelization without any granulation tissue or inflammation. Treatment with AgNPs resulted in negative immunostaining of tumor necrosis factor-α. The levels of the inflammation markers, interleukin-6 and interleukin-1β, significantly decreased (p < 0.05) in the AgNPs-treated group. There was also a pronounced increase in the gene expression of fibronectin and platelet-derived growth factor in the wound tissues. Thus, AgNPs synthesized using ELLE may be a promising antifungal and wound healing agent.

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Irisin/PGC-1α/FNDC5 pathway in Parkinson’s disease: truth under the throes

Ali,

Alhamdan,

Al-kuraishy

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Antibacterial activity of nano zinc oxide green-synthesised from Gardenia thailandica triveng. Leaves against Pseudomonas aeruginosa clinical isolates: in vitro and in vivo study

Cited by 41 publications

References 36 publications

Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

Anti-Biofilm and Antibacterial Activities of Cycas media R. Br Secondary Metabolites: In Silico, In Vitro, and In Vivo Approaches

Silver Nanoparticles Prepared Using Encephalartos laurentianus De Wild Leaf Extract Have Inhibitory Activity against Candida albicans Clinical Isolates

Irisin/PGC-1α/FNDC5 pathway in Parkinson’s disease: truth under the throes

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