Antibacterial Activity of Mouthwash Incorporated with Silica Nanoparticles against S. aureus,  S. mutans, E. faecalis: An in-vitro Study

et al. 2021

JPRI

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Introduction: Mucuna pruriens is a tropical legume native to Africa and tropical Asia. The plant is an annual climbing shrub with long vines that can reach a height of 15 meters in length. Mucuna pruriens belong to the family legume. It is a herbal drug that is used as a medicine for the treatment of male infertility, nervous disorder, and also as an aphrodisiac. It has been shown that it potentially has more medical importance. It causes orange hair that causes a severe itch when in contact with the skin. It releases a protein called mucanain. Aim: To determine the anti-inflammatory activity of aqueous and hexane extract of Mucuna pruriens. Materials and Methodology: The extract was prepared and the anti-inflammatory effect was done using a UV-Beckmann spectrometer. The anti-inflammatory activity of nanoparticles prepared using plant extract was investigated by albumin denaturation assay. The results of the test were described as the standard deviation and analyzed using UV-Vis spectroscopy. Correlation and analysis were done in SPSS. Results: The hexane solution and aqueous solution of Mucuna pruriens have a high percentage of inhibition at the 30 μl, 40 μl and 50 μl concentration when compared with a standard solution. And hexane has a high percentage of inhibition at 50μl when compared with a standard solution. The obtained data were analyzed using spearman correlation analysis and the non-parametric correlation was statistically significant at p value< 0.05. Conclusion: The aqueous and hexane extract of Mucuna pruriens show good anti- inflammatory activity. In that hexane extract has good anti-inflammatory activity when compared with aqueous extract. And it is used for disease. And further research should be done with the action and mechanism of Mucuna pruriens.

Section: Fig 8 the Line Graph Shows The Percentage Of Inhibition And Absorbance Levels At Different Concentrations Of The Extract In Thismentioning

confidence: 89%

Comparative Anti Inflammatory Activity of Hexane and Aqueous Extract of Mucuna pruriens

Divyashri

Priyadharshini

et al. 2021

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“…In sol-gel reaction, there is hydrolysis and condensation of Tetra-ethoxy silane, where it was first hydrolyzed to silicic acid, followed by a condensation reaction leading to the formation of Si-O-Si bonds. Silica nanoparticles incorporated in mouthwash showed a good potential as an antimicrobial agent that maintains control of biofilm, preventing initial colonization of bacteria [42].…”

Section: Fig 2 Uv Spectroscopic Analyses Of Zinc Oxide Nanoparticles ...mentioning

confidence: 99%

Antimicrobial Activity of Zinc Oxide Nanoparticles Synthesized Using Leaves Extract of Abies webbiana

Nivethitha

Jeevitha

et al. 2021

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Introduction: Green synthesis of nanoparticles using plant extract, bacteria, fungi and enzymes are eco-friendly and cost effective which do need high pressure, energy, temperature and toxic chemicals for its synthesis protocol. Zinc oxide nanoparticles have various physical, chemical and biological properties which can be applied in the treatment of oral diseases. Aim: The main objective of the current study was to synthesize the zinc oxide nanoparticles (ZnO NPs) in a cost effective biological method. The extract of A. webbiana was used to synthesis Zinc oxide nanoparticles. Materials and Methods: In this report, we used the extract of A. webbiana to synthesize zinc oxide nanoparticles and were characterized using U-V visible spectrophotometer and were tested for their antimicrobial activity against oral pathogens. Results: These biosynthesized zinc oxide nanoparticles exhibited potent antimicrobial activity against oral pathogens, Streptococcus mutans, Staphylococcus aureus and Enterococcus faecalis, Candida albicans and can be developed as a novel medicine against pathogenic oral diseases. Abies webbiana extract mediated zinc oxide nanoparticles showed effective antimicrobial activity comparable to others. Conclusion: With increasing demand in advancements in diagnosis and treatment modalities, green synthesis of zinc oxide nanoparticles using A. webbiana which was found to have potent antimicrobial action has wider applications in dentistry. This formulation needs to be further purified to isolate the component in the extract that makes it effective to be used in large scale production for targeted drug delivery against a wide array of oral microbial infections.

“…Nanotechnology deals with particles which are less than 100 nm in size and have important roles in medicines, industries and drug gene delivery systems. Nanomaterials are particles having nanoscale measurement and they are estimated as small particles with upgraded synergist reactivity, warm conductivity, nonstraight optical execution and compound relentlessness attributable to its enormous surface territory to its volume proportion [1,2]. Nanoparticles have begun being considered as nano antibiotics in light of their antimicrobial activities [3].In today's world, nanoparticles have been incorporated into different modern, wellbeing, nourishment, feed, space, compound, and beauty care products which requires a green and condition cordial way to deal with their synthesis [1].…”

Section: Introductionmentioning

confidence: 99%

“…Nanomaterials are particles having nanoscale measurement and they are estimated as small particles with upgraded synergist reactivity, warm conductivity, nonstraight optical execution and compound relentlessness attributable to its enormous surface territory to its volume proportion [1,2]. Nanoparticles have begun being considered as nano antibiotics in light of their antimicrobial activities [3].In today's world, nanoparticles have been incorporated into different modern, wellbeing, nourishment, feed, space, compound, and beauty care products which requires a green and condition cordial way to deal with their synthesis [1]. The size of the nanoparticles is similar to most of the biological molecules and structures therefore the nanoparticles can be used for both in vitro and in vivo biomedical research and applications [4,5].…”

Section: Introductionmentioning

confidence: 99%

“…The use of Ag NPs is increasing because of their unique properties like high electrical conductivity, chemical stability, catalytic and antimicrobial activity [9]. The size, shape and surface chemistry of AgNPs have an effect on their physical, chemical, optical and electrical properties and Small sized Ag NPs are of great use because of their potential applications in emerging biomedical, antibacterial, antifungal and antiviral areas [1,10].…”

Section: Introductionmentioning

confidence: 99%

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Preparation of Mouthwash using Ficus benghalensis Assisted Silver Nanoparticles and a Comparative Analysis of Its Antimicrobial Activity

Rangeela

Jeevitha

et al. 2021

JPRI

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Aim: The aim of this study is to prepare a mouthwash using Ficus benghalensis mediated silver nanoparticles (Ag NPs) and analysing its antimicrobial activity against oral pathogens Introduction: Nanotechnology deals with the particles which are less than 100 nm and have important roles in medicines, industries, drug gene delivery. Different parts of the F. benghalensis show medicinal properties. Leaves are used for ulcers, aerial roots are used in gonorrhoea, seeds and fruits are used for dysentery, diarrhoea and diabetes. F. benghalensis have been reported to have immunomodulatory, antibacterial activity and are used to treat toothache. Materials and methods: In the present study, the silver nanoparticles were synthesized from Ficus benghalensis plant extract. The preparation of plant extract was done using commercially available F. benghalensis leaves powder for the synthesis process. The mouthwash was prepared using 1 mM of silver nitrate. The prepared nanoparticles were confirmed using a UV-vis spectrophotometer. Different concentrates of the synthesised Ficus benghalensis mediated silver nanoparticles were tested against Staphylococcus aureus, Streptococcus mutans, Enterococcus faecalis and Candida albicans using agar well diffusion method and MIC will be determined. Results: It has been observed that the antimicrobial effect of the prepared Ag NPs was almost similar against all the organisms used in the study with a maximum zone of inhibition against Staphylococcus aureus. It has also been observed that the antimicrobial activity of the AgNPs increased with increasing concentration. Conclusion: As the synthesised F. benghalensis assisted silver nanoparticle mouthwash showed potent antimicrobial activity in vitro against the oral microbes: S. aureus, S. mutans, E. faecalis, C. albicans, in vivo studies are needed further to evaluate the antimicrobial effect.